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Redder MMP Substrates. Better Activity Assays.

Extensive MMP Product Range

Proteins of the matrix metalloproproteinase (MMP) family are collectively involved in the breakdown of extracellular matrix proteins and processing of bioactive molecules and are required for some normal processes. Unfortunately, these proteins are also associated with many pathological processes including cirrhosis, arthritis, metastasis, and chronic cardiovascular diseases. Collagenases (Matrix Metalloproteinase-1, MMP-8, and MMP-13) and gelatinases (MMP-2 and MMP-9) also break down skin collagens, contributing to aging and wrinkling. Enzo offers high sensitivity colorimetric and fluorometric activity assays for screening collagenase and gelatinase inhibitors as well as highly active enzymes and specific antibodies for all your MMP research needs.

	Product Types
	Assay Kits	Substrates	Active Proteins	Antibodies	Inhibitors
Broad Spectrum MMP Analysis	Find	Find	Find		Small Molcules / TIMPs
MMP-1	Find	Find	Find	Find
MMP-2	Find	Find	Find	Find	Find
MMP-3	Find	Find	Find	Find	Find
MMP-7	Find	Find	Find	Find
MMP-8	Find	Find	Find
MMP-9	Find	Find	Find	Find
MMP-10	Find	Find	Find	Find
MMP-11		Find	Find
MMP-12	Find	Find	Find	Find
MMP-13	Find	Find	Find	Find
MMP-14	Find	Find	Find
MMP-19	Find	Find	Find
MMP-20	Find	Find	Find
MMP-24		Find	Find

Ultra-sensitive, long wavelength fluorogenic substrates reduce interference.

The MMP RED and GREEN Drug Discovery Kits are complete assay systems designed to screen MMP inhibitors, using long-wavelength quenched fluorogenic substrates. The assays are performed in a convenient 96-well microplate format, with the compound NNGH [1] also included as a prototypic control inhibitor.

Improved red-shifted substrate with better kinetics and brighter signal
Includes active recombinant enzyme, substrate, and assay buffer
Convenient real-time kinetics of cleavage is easily determined
Microplate format for high-throughput screening

Figure 1: Fluorescent emission spectra of small molecule drug camptothecin (light blue), OE33 cells (dark blue), MMP GREEN substrate (green), and MMP RED substrate (red), showing that the MMP GREEN and RED substrates avoid the interference caused by small molecules and cells.

OMNIMMP™ RED and MMP-3 Fluorogenic substrates offer key advantages over other MMP substrates

Emission at the higher end of the spectrum (576nm and 521nm, respectively, after excitation at 545nm and 494nm) avoids the interference at lower wavelengths often exhibited by screening compounds, and by substances commonly found in biological samples and tissue culture medium.
Highly quenched (very low background), but once cleaved by MMPs emits extremely bright signal.
MMP substrate peptides inherently display poor aqueous solubility, often with KmS near their limits of solubility, making enzyme and inhibitor kinetics difficult. MMP K_mS for OMNIMMP™ RED and MMP-3 Fluorogenic Substrates are well below the solubility limits of the substrates.
In addition to the efficient binding as exhibited by low KmS, OMNIMMP™ RED and MMP-3 Fluorogenic Substrates are avidly cleaved by MMPs, with k_mt/K_catS in the range of 10⁵-10⁷ M^-1sec^-1.
Better kinetics allows lower substrate concentrations, avoiding inhibition by the substrate or competition with the inhibitor at the active site.
The ultra-strong fluorescence of OMNIMMP™ RED and MMP-3 Fluorogenic Substrates allows for substrate concentrations much lower than the K_m, a condition generally desirable in inhibitor screening assays.

OMNIMMP® RED fluorogenic substrate (µM)

Figure 2: Determination of k_cat/K_m for MMP-1 using OMNIMMP™ RED. K_m = 0.48 µM,

Percentage of inhibition (%)

Figure 3: Inhibition of ten MMPs by 1.3 µM NNGH.

Featured Products

	Matrix metalloproteinase-1 (MMP-1) fluorometric drug discovery kit, RED
	Fluorescent detection, HTS, Activity assay \| Print as PDF

	BML-AK301-0001	96 wells	714.00 USD
Do you need bulk/larger quantities?

	Matrix metalloproteinase-2 (MMP-2) fluorometric drug discovery kit, RED
	Fluorescent detection, HTS, Activity assay \| Print as PDF

	BML-AK302-0001	96 wells	714.00 USD
Do you need bulk/larger quantities?

	Matrix metalloproteinase-3 (MMP-3) fluorometric drug discovery kit, GREEN
	Fluorescent detection, HTS, Activity assay \| Print as PDF

	BML-AK303-0001	96 wells	714.00 USD
Do you need bulk/larger quantities?

	Matrix metalloproteinase-3 (MMP-3) fluorometric drug discovery kit, RED
	Fluorescent detection, HTS, Activity assay \| Print as PDF

	BML-AK311-0001	96 wells	693.00 USD
Do you need bulk/larger quantities?

	Matrix metalloproteinase-7 (MMP-7) fluorometric drug discovery kit, RED
	Fluorescent detection, HTS, Activity assay \| Print as PDF

	BML-AK304-0001	96 wells	714.00 USD
Do you need bulk/larger quantities?

	Matrix metalloproteinase-9 (MMP-9) fluorometric drug discovery kit, RED
	Fluorescent detection, HTS, Activity assay \| Print as PDF

	BML-AK306-0001	96 wells	714.00 USD
Do you need bulk/larger quantities?

	Matrix metalloproteinase-12 (MMP-12) fluorometric drug discovery kit, GREEN
	Fluorescent detection, HTS, Activity assay \| Print as PDF

	BML-AK312-0001	96 wells	693.00 USD
Do you need bulk/larger quantities?

	Matrix metalloproteinase-19 (MMP-19) fluorometric drug discovery kit, RED
	Fluorescent detection, HTS, Activity assay \| Print as PDF

	BML-AK307-0001	96 wells	693.00 USD
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	Matrix metalloproteinase (MMP) inhibitor profiling kit, fluorometric RED
	Fluorescent detection, HTS, Activity assay \| Print as PDF

	BML-AK308-0001	96 wells	1,273.00 USD
Do you need bulk/larger quantities?

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