AMPIGENE® Taq Mixes and DNA Polymerases

Our AMPIGENE® Taq Mixes and DNA Polymerases all include stabilizers, enhancers, buffers, polymerases, dNTPs, and magnesium to enable easy standard PCR. The AMPIGENE® HS Taq Mixes and HS DNA Polymerase allow for more sample types and increase PCR sensitivity.

AMPIGENE® 1-Step RT-PCR Kit

Convenient and easy, our AMPIGENE® 1-Step RT-PCR Kit has all the components for efficient cDNA synthesis and PCR in one tube. The MMLV RTase is thermostable and extremely active, allowing efficient cDNA synthesis, while the buffer contains enhancers to prevent the formation of primers/dimers to increase sensitivity and specificity.

AMPIGENE® qPCR Mixes

Using the latest developments in polymerase technology and buffer chemistry, AMPIGENE® qPCR Green Mixes and AMPIGENE® qPCR Probe Mixes require minimal optimization to deliver high performing real-time PCR. For ease of use, the qPCR Mixes come premixed with a range of different ROX reference dye intensities in order to suit your qPCR instrumentation.

AMPIGENE® qPCR 1-Step Kits

Our AMPIGENE® qPCR 1-Step Kits contain components needed for convenient cDNA synthesis and qPCR in one tube. The MMLV RTase is optimized for efficient cDNA synthesis while the qPCR components allow sensitive quantitation. Choose the convenient AMPIGENE® qPCR 1-Step Kit in the optimized reference dye format for your qPCR needs.

Higher Yield

450bp fragment of the human myc gene was amplified with AmpiGene® Taq DNA Polymerase and compared with Taq Polymerases from other suppliers. A serial dilution of human genomic DNA was incubated under standard PCR conditions. AmpiGene® Taq delivers higher yield and sensitivity as compared with all six competing products.

Higher Sensitivity

Human Genomic DNA 100ng/µl, then 1/3 serial dilution A) β2MG (Beta-2 microglubulin) gene B) pgk gene. AmpiGene® HS Taq DNA Polymerase does not produce primer-dimers and can amplify lower concentrations of DNA even from genes such as pgk that have high secondary structure levels.

The PCR Decontamination Kit removes DNA contamination from PCR mastermixes. Contaminating DNA can be removed in the presence of primers and probe by utilizing a specific dsDNase. The dsDNase is irreversibly inactivated by heating, leaving the mastermix components unaffected.

• Reduce background to improve target detection
• No effect on PCR sensitivity
• Easy protocol – decontaminate directly in mastermix

The gDNA Removal Kit removes DNA contamination from RNA prior to reverse transcription.  It uses a heat labile dsDNase which is irreversibly inactivated at low temperatures. This enables an inactivation step which is gentle enough to preserve both quality and quantity of all present RNA.

• Removes gDNA from RNA prior to reverse transcription in the same tube
• HL-dsDNase is inactivated without reducing quality or quantity of RNA
• Minimizes pipetting steps and suitable for high throughput experiments

Efficient removal of gDNA without affecting RNA



The gDNA Removal Kit removes gDNA from RNA preps to levels below the detection limit but does not affect RNA. Compared to untreated control, no reduction in cDNA level (SDHA) was observed RT-qPCR.

PCR & Gel clean-up columns use silica membrane technology in a mini spin column format to deliver easy and efficient DNA purification from PCR products, agarose gels, and labeling reactions.

• High recoveries for fragments down to 50 bp
• Small elution volume down to 15 µl
• Binding buffer with pH indicator

Shrimp Alkaline Phosphatase, (recombinant), is a heat labile alkaline phosphatase that can be used in a PCR clean-up application to dephosphorylate nucleotides. It is fully inactivated by a short heat treatment to simplify workflows.

• Completely heat inactivated after 5 min at 65°C
• Highly stable at room temperature and 4°C

Rapid and complete heat inactivation



Heat inactivation of Shrimp Alkaline Phosphatase at 65°C and 75°C