Next Generation Sequencing

Tools to Streamline Your NGS Workflow

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Reliable and Streamlined NGS Workflow

Nucleic Acid Extraction

Library Preparation

Related Products

Tools to Streamline Your NGS Workflow

Next generation sequencing (NGS) is a method of high-throughput sequencing in which millions of DNA strands can be sequenced in parallel. Many researchers use NGS as an important tool to gain insight into the detailed and comprehensive genetic and epigenetic modifications of genomes from many different cell types. Choose from our comprehensive mix of kits to extract and prepare your library sample for NGS analysis.

Whether you are preparing samples for ChIP-seq, whole genome sequencing, or Bisuilfite-sequencing our kits are easy, efficient, and convenient.

Reliable and Streamlined NGS Workflow

Nucleic Acid Extraction

AMPIXTRACT® Solutions

Library Preparation

AMPINEXT™ Products

Sequencing & Analysis

Compatible with Illumina® Platform

Nucleic Acid Extraction

Fast – Entire procedure can be finished in as little as 15 minutes with consistent isolation conditions
Effective – High efficiency of nucleic acid isolation from various sample types with low amounts of start material (as low as 1 ng)
Reliable – Our specially designed columns allow for nucleic acids to be conveniently recovered
Safe – Use of non-toxic reagents and no phenol chloroform

Product Name	Sample Input	Elution Volume	Protocol Time
AMPIXTRACT® General Tissue Section DNA Isolation Kit	1 ng (optimal range 10 ng – 1 μg)	8 - 20 μL	2 hours
AMPIXTRACT® Paraffin Tissue Section DNA Isolation Kit	1 ng (optimal range 10 ng – 1 μg)	8 - 20 μL	2 hours
AMPIXTRACT® Urine DNA Isolation Kit	1 ng – 2 μg (optimal range 10 ng – 1 μg)	8 - 20 μL	20 minutes
AMPIXTRACT® Blood and Cultured Cell DNA Extraction Kit	1 ng – 4 μg (optimal range 10 ng – 1 μg)	10 - 20 μL	20 minutes

Library Preparation

Fast and Streamlined Procedure – Compact protocols with simple clean-up steps to save time and prevent handling errors, as well as loss of valuable samples
Highly Convenient – The kit contains all required components for each step of the library preparation for the most reliable and consistent results
Minimize bias – High quality enzymes achieve reproducibly high yields of nucleic acid library with minimal sequence bias and low error rates
Flexibility – Can be used for both non-barcoded (singleplexed) and barcoded (multiplexed) library preparation with broad range of nucleic acid input

Product Name	DNA		Bisulfite-Seq			Post Bisulfite-Seq	ChIP-Seq
Product Name	AMPINEXT™ DNA Library Preparation Kit	AMPINEXT™ High-Sensitivity DNA Library Preparation Kit	AMPINEXT™ High-Sensitivity Bisulfite-Seq Kit	AMPINEXT™ RNA Bisulfite-Seq Kit	AMPINEXT™ 5-mC RNA Bisulfite-Seq Easy Kit	AMPINEXT™ Post-Bisulfite DNA Library Preparation Kit	AMPINEXT™ ChIP-Seq High-Sensitivity Kit
Input Type	DNA	DNA	DNA	RNA	RNA	DNA	Cells	Tissues
Input Range	5 ng – 1 μg	0.2 ng – 100 ng	0.2 ng – 200 ng	5 ng – 1 μg	5 ng – 500 ng	0.5 ng – 1 μg	10⁵ – 10⁶ Cells	5 mg – 50 mg
(Optimal Range)	(100 ng – 200 ng)	(10 ng – 50 ng)	(10 ng – 50 ng)	(200 ng – 500 ng)	(100 ng – 200 ng)	(100 ng – 200 ng)	(4×10⁵ – 5×10⁵ Cells)	20 mg – 30 mg)
Protocol Time	< 2.5 hours	< 1.5 hours	< 6.5 hours	6 hours		5 hours	< 7 hours

Related Products

Product Name	Contents
AMPINEXT™ DNA Size Selection Kit	MQ Binding Beads, Elution Solution, 96-well plate
AMPINEXT™ NGS Barcode (Index) Set-12	AMPINEXT™ Barcodes 1-12 (10 μM) AMPINEXT™ Universal Primer (10 μM)