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DNA Damage & Content Analysis

DNA Damage ELISA Kit

The DNA Damage ELISA (enzyme-linked immunosorbent assay) is a fast and sensitive immunoassay providing results in less than 2.5 hours. Quantitation of 8- hydroxy-2’-deoxyguanosine (8-OHdG) in urine, serum, and saliva samples is performed in a convenient 96-well plate format using a colorimetric substrate. 8-OHdG is a frequently-used critical biomarker of oxidative stress and carcinogenesis.

Quantify levels < 1 ng/ml
Validated in-house in a variety of sample matrices
Tested in a variety of biofluids (urine, serum, and saliva)
Convenient colorimetric 96-well plate format

Typical 8-OHdG Standard Curve

Figure 1: The standard curve has a range of 0.94 – 60 ng/mL.

COMET SCGE Assay Kit

Sensitive and versatile method for measuring single- and double-strand DNA breaks in individual cells.
Exposure of cells to oxidative and environmental stresses frequently results in the breakdown or oxidation of genomic DNA. Assays to evaluate the integrity of genomic DNA, or to assess the presence of oxidized DNA are frequently used as a means of verifying the onset of apoptosis or DNA damage. The Assay Designs COMET SCGE Assay measures DNA damage by fluorescently detecting the integrity of DNA liberated from cells embedded in low melting point agarose. Upon electrophoresis, fragmented DNA produces a characteristic “comet” shaped tail as small DNA fragments migrate in the gel more rapidly than in-tact genomic DNA.

The COMET SCGE Assay is a fast and simple electrophoresis method to detect and quantitate DNA fragmentation in cells associated with DNA damage and apoptosis. A unique nucleic acid stain provides improved sensitivity for DNA visualization compared to ethidium bromide.

Ready-to-use Comet Slides allow direct application of sample without pretreatment
Shorter assay time allows for higher throughput sample analysis
Hydrophobic barrier allows sample treatment with DNA repair enzymes
Unique nucleic acid stain provides improved sensitivity for DNA visualization compared to ethidium bromide

NUCLEAR-ID® Cell Cycle Analysis Kits

Highly permeable fluorescent dyes for DNA content analysis in live or fixed cells.

Intercalating dye with superior permeability in live cells
Dye functional over a wide range of cell densities, incubation time, and temperature eliminating optimization required with other dyes
Easy no-wash, mix and read protocol
Dyes excitable with standard 488nm laser

Figure 1. G2/M accumulation of Nocodazole-treated cells using Nuclear-ID dye in live cells.

References:

Houben R, et.al. An intact retinoblastoma protein binding site in merkel cell polyomavirus large T antigen is required for promoting growth of merkel cell carcinoma cells. Int J Cancer. 2011 Mar 16. doi: 10.1002/ijc.26076 GFP-certified® Nuclear-ID® Red Cell Cycle Analysis Kit
Xiang, Y, et.al. A cell-permeant dye for cell cycle analysis by flow and laser-scanning microplate cytometry. Nature Methods 6, (2009) (Advertising Feature) Nuclear-ID® Red Cell Cycle Analysis Kit, GFP-certified®

Featured Products

	Comet SCGE assay kit
	Fast and simple electrophoresis method to measure DNA fragmentation
	Fluorescence microscopy \| Print as PDF

	ADI-900-166	50 tests	375.00 USD
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	DNA damage ELISA kit
	Rapid DNA damage ELISA kit for cancer, apoptosis and oxidative stress.
	ELISA, Colorimetric detection \| Print as PDF

	ADI-EKS-350	96 wells	958.00 USD
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