Cytotoxicity is measured primarily as damage to living cells and initiation of cell death and presents in similar ways across cell types. Hepatocytes are important in predictive toxicity studies due to the metabolic drug activities that make them more susceptible to drug compounds. Cell death can be determined by measuring the leakage of cell components, presenting information about membrane integrity and dying cells' mechanism. Standard measures of cell death include lactate dehydrogenase (LDH), DNA binding dyes, or measuring dead cell-specific protease activity. Cell viability can also be used in tandem with cell death markers, most commonly tested by measuring ATP (a measure of living and competent cells). Application NoteThree Dimensional Hepatotoxicity Screening using Corning® HepatoCells, the Spheroid Microplate, and the SCREEN-WELL® Hepatotoxicity Library |
Download Our Predictive Toxicology Brochure 
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A rapid, quantitative live cell cytotoxicity assay compatible with high-throughput screening (HTS)
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Monitor Dysfunction of Lysosomal Degradation
Drug-induced lysosome accumulation in U-2 OS cells was evaluated using Verapamil (200 µM) and LYSO-ID® Red dye. |
LYSO-ID® Red cytotoxicity kit (GFP-CERTIFIED®) |
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| A Rapid, Quantitative and HTS‐compatible Live Cell Cytotoxicity Assay | ||||||
| Fluorescence microscopy, Fluorescent detection | Print as PDF | ||||||
| ENZ-51015-KP002 | 1 Kit | 417.00 USD |  |
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NUCLEAR-ID® Blue/Green cell viability reagent |
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| Distinguishes between live vs dead cells in a single reagent. | ||||||
| ≥93% (HPLC), Flow Cytometry, Fluorescence microscopy, Fluorescent detection | Print as PDF | ||||||
| ENZ-53004-C100 | 100 µl | 316.00 USD | |
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| Do you need bulk/larger quantities? | ||||||
NUCLEAR-ID® Blue/Red cell viability reagent (GFP-CERTIFIED®) |
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| Distinguishes between live vs dead cells in a single reagent. | ||||||
| ≥93% (HPLC), Flow Cytometry, Fluorescence microscopy, Fluorescent detection | Print as PDF | ||||||
| ENZ-53005-C100 | 100 µl | 304.00 USD | |
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| Do you need bulk/larger quantities? | ||||||
NUCLEAR-ID® Red/Green cell viability reagent |
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| Distinguishes between live vs dead cells in a single reagent | ||||||
| ≥93% (HPLC), Flow Cytometry, Fluorescence microscopy, Fluorescent detection | Print as PDF | ||||||
| ENZ-53006-C100 | 100 µl | 310.00 USD | |
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| Do you need bulk/larger quantities? | ||||||
SCREEN-WELL® Hepatotoxicity library |
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| Essential standards for predictive toxicology screening | ||||||
| HTS | Print as PDF | ||||||
| BML-2851-0100 | 1 Library | 100 µl/well | 4,335.00 USD | |
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| BML-2851-0500 | 1 Library | 500 µl/well | 13,056.00 USD | |
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Cytochrome P450 1A1 (rat) polyclonal antibody |
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| IHC (FS), IHC (PS), WB | Print as PDF | ||||||
| BML-CR3110-0025 | 25 µl | 105.00 USD | |
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Cytochrome P450 1A2 (human) polyclonal antibody |
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| IHC (FS), IHC (PS), WB | Print as PDF | ||||||
| BML-CR3130-0025 | 25 µl | 119.00 USD | |
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| Do you need bulk/larger quantities? | ||||||
Cytochrome P450 2C8, 2C9, 2C19 (human) and 2C12 (rat) polyclonal antibody |
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| IHC (FS), IHC (PS), WB | Print as PDF | ||||||
| BML-CR3280-0025 | 25 µl | 119.00 USD | |
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| BML-CR3280-0100 | 100 µl | 381.00 USD | |
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Cytochrome P450 2D4 (rat) polyclonal antibody |
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| IHC (FS), IHC (PS), WB | Print as PDF | ||||||
| BML-CR3240-0025 | 25 µl | 105.00 USD | |
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| BML-CR3240-0100 | 100 µl | 381.00 USD | |
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Cytochrome P450 3A1 (rat) polyclonal antibody |
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| IHC (FS), IHC (PS), WB | Print as PDF | ||||||
| BML-CR3315-0025 | 25 µl | 105.00 USD | |
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Cytochrome P450 3A4 polyclonal antibody |
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| WB | Print as PDF | ||||||
| ALX-210-276-R100 | 100 µl | 782.00 USD | |
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Cytochrome P450 polyclonal antibody |
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| Purified from rabbit serum., WB | Print as PDF | ||||||
| ADI-MFO-100-D | 50 µg | 195.00 USD | |
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| ADI-MFO-100-F | 200 µg | 427.00 USD | |
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Cytochrome P450 Reductase polyclonal antibody |
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| Purified from rabbit serum., ICC, IP, WB, Inhibition assay | Print as PDF | ||||||
| ADI-OSA-300-D | 50 µg | 236.00 USD | |
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| ADI-OSA-300-F | 200 µg | 513.00 USD | |
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3-Cyano-7-ethoxycoumarin |
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| Fluorescent substrate for Cytochrome P450 | ||||||
| 117620-77-6, ≥98% (TLC) | Print as PDF | ||||||
| ENZ-CHM264-0010 | 10 mg | 112.00 USD | |
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| ENZ-CHM264-0050 | 50 mg | 477.00 USD | |
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1-Aminobenzotriazole |
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| Cytochrome P450 inhibitor | ||||||
| 1614-12-6, ≥98% (GC) | Print as PDF | ||||||
| BML-EI259-0100 | 100 mg | 165.00 USD | |
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| Do you need bulk/larger quantities? | ||||||
3,3’-Diindolylmethane |
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| Cytochrome P450 activator | ||||||
| 1968-05-4, ≥98% (HPLC) | Print as PDF | ||||||
| ALX-350-370-M500 | 500 mg | 77.00 USD | |
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| Do you need bulk/larger quantities? | ||||||
7-Ethoxyresorufin |
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| Cytochrome P450 substrate | ||||||
| 5725-91-7, ≥98% | Print as PDF | ||||||
| BML-EI262-0010 | 10 mg | 263.00 USD | |
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| Do you need bulk/larger quantities? | ||||||
HET-0016 |
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| Cytochrome P450 inhibitor | ||||||
| 339068-25-6, ≥98% (HPLC) | Print as PDF | ||||||
| BML-EI350-0005 | 5 mg | 113.00 USD | |
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| BML-EI350-0025 | 25 mg | 449.00 USD | |
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Itraconazole |
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| CYP 14-α demethylase inhibitor. Autophagy inducer. | ||||||
| 84625-61-6, ≥98% (HPLC) | Print as PDF | ||||||
| ENZ-CHM265-0050 | 50 mg | 67.00 USD | |
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| ENZ-CHM265-0250 | 250 mg | 253.00 USD | |
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Ketoconazole |
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| Cytochrome P450 inhibitor | ||||||
| 65277-42-1, ≥99% (HPLC) | Print as PDF | ||||||
| BML-EI107-0050 | 50 mg | 70.00 USD | |
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| BML-EI107-0500 | 500 mg | 342.00 USD | |
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| Do you need bulk/larger quantities? | ||||||
Methoxsalen |
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| Cytochrome P450 inhibitor | ||||||
| 298-81-7, ≥98% (GC) | Print as PDF | ||||||
| BML-EI132-0001 | 1 g | 65.00 USD | |
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| Do you need bulk/larger quantities? | ||||||
Metyrapone |
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| Cytochrome P450 inhibitor | ||||||
| 54-36-4, ≥99% | Print as PDF | ||||||
| BML-EI256-0200 | 200 mg | 44.00 USD | |
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Muscone |
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| Induces rat liver microsomal cytochrome P-450 | ||||||
| 541-91-3, ≥99% (TLC) | Print as PDF | ||||||
| BML-EI161-0500 | 500 mg | 338.00 USD | |
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| Do you need bulk/larger quantities? | ||||||
Omeprazole |
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| CYP2C19 inhibitor. H+/K+ ATPase inhibitor. | ||||||
| 73590-58-6, ≥98% (TLC) | Print as PDF | ||||||
| ENZ-CHM267-0050 | 50 mg | 67.00 USD | |
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| ENZ-CHM267-0500 | 500 mg | 281.00 USD | |
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Piperine |
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| CYP3A4 inhibitor. Natural product. | ||||||
| 94-62-2, ≥98% (HPLC) | Print as PDF | ||||||
| ENZ-CHM268-0050 | 50 mg | 50.00 USD | |
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| ENZ-CHM268-0250 | 250 mg | 169.00 USD | |
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R-(-)-Glucoraphanin . potassium salt |
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| Cytochrome P450 modulator | ||||||
| 21414-41-5, Isolated from Brassica oleracea var. palmifolia, ≥99% (HPLC) | Print as PDF | ||||||
| ALX-350-181-M010 | 10 mg | 350.00 USD | |
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| Do you need bulk/larger quantities? | ||||||
Ritonavir |
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| CYP3A4 inhibitor. HIV1/2 protease inhibitor. | ||||||
| 155213-67-5, ≥98% (HPLC) | Print as PDF | ||||||
| ENZ-CHM266-0010 | 10 mg | 67.00 USD | |
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| ENZ-CHM266-0050 | 50 mg | 253.00 USD | |
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Roquefortine C |
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| Cytochrome P450 inhibitor | ||||||
| 58735-64-1, Isolated from MST-FP1764., ≥95% (HPLC) | Print as PDF | ||||||
| ALX-350-342-MC05 | 0.5 mg | 522.00 USD | |
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| Do you need bulk/larger quantities? | ||||||
SKF-525A . HCl |
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| Cytochrome P450 inhibitor | ||||||
| 62-68-0, ≥95% (TLC) | Print as PDF | ||||||
| BML-EI105-0250 | 250 mg | 108.00 USD | |
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| BML-EI105-1000 | 1 g | 318.00 USD | |
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| Do you need bulk/larger quantities? | ||||||
(S)-Mephenytoin |
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| Cytochrome P450 substrate | ||||||
| 70989-04-7, ≥98% (HPLC) | Print as PDF | ||||||
| BML-J120-0005 | 5 mg | 102.00 USD | |
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| BML-J120-0025 | 25 mg | 429.00 USD | |
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| Do you need bulk/larger quantities? | ||||||
