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Cell Death, Apoptosis, or Necrosis

Visualize Toxic Events In Vitro


Apoptosis, or programmed cell death, is naturally occurring and activated by caspases that cleave proteins. Caspases are classified as effector or initiator caspases and can be used to determine apoptosis. Necrosis is an alternative to apoptosis and is not a regulated process. Injury, diseases, or external factors results in premature cell death that triggers necrosis. Apoptosis and necrosis can be differentiated by evaluating caspase 3/7 pathway activation.


TechNote

How to Detect Key Features of Apoptosis?

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Product Highlight

GFP-CERTIFIED® Apoptosis/Necrosis detection kit


GFP-compatible multiplex assay for distinguishing between healthy, early apoptotic, late apoptotic, and necrotic cells

  • True multiplexing capabilities with GFP and other green fluorescent
  • Optimized for both fluorescence microscopy and flow cytometry applications
  • Suitable for death pathway analysis and drug/toxin studies

Dual Detection of Apoptotic and Necrotic Cells



Apoptosis Detection Reagent (Gold) and Necrosis Detection Reagent (Red) specifically detect cell state with clear spectral separation from mitochondria-associated GFP signal (Green).

Solutions for Cell Death, Apoptosis, or Necrosis Toxicity

APO-BRDU™ kit 

TUNEL assay for the detection of apoptosis by flow cytometry
Flow Cytometry | Print as PDF
 
ALX-850-042-KI01 1 Kit 783.00 USD
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GFP-CERTIFIED® Apoptosis/Necrosis detection kit 

Multiplex assay that distinguishes between healthy, early apoptotic, late apoptotic and necrotic cells, compatible with GFP and other fluorescent probes (blue or cyan)
Flow Cytometry, Fluorescence microscopy, Fluorescent detection | Print as PDF
 
ENZ-51002-25 25 assays 199.00 USD
 
ENZ-51002-100 100 assays 463.00 USD
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ApoSENSOR™ Cell viability assay kit 

A simple no-wash, chemiluminescent cell  viability assay
Chemiluminescence | Print as PDF
 
ALX-850-247-KI01 200 tests 312.00 USD
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Calcein AM (ultra pure) 

Calcium dye
148504-34-1, ≥95% (HPLC) | Print as PDF
 
ENZ-52002 1 mg 171.00 USD
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Cell Counting Kit-8 

Simple, fast and accurate assay to measure cell proliferation and cytotoxicity
Colorimetric detection | Print as PDF
 
ALX-850-039-0100 100 tests 100.00 USD
 
ALX-850-039-KI01 500 tests 216.00 USD
 
ALX-850-039-KI02 5x500 tests 641.00 USD
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Cell Counting Kit-F 

Fluorescence microscopy | Print as PDF
 
ALX-850-245-KI01 500 tests 216.00 USD
 
ALX-850-245-KI02 5x500 tests 790.00 USD
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Comet SCGE assay kit 

Fast and simple electrophoresis method to measure DNA fragmentation
Fluorescence microscopy | Print as PDF
 
ADI-900-166 50 tests 365.00 USD
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Cytochrome c (human), ELISA kit 

ELISA, Colorimetric detection | Print as PDF
 
ADI-900-141 96 wells Inquire for pricing
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Cytochrome c releasing apoptosis assay kit 

Print as PDF
 
ALX-850-277-KI01 1 Kit 823.00 USD
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CYTO-ID® Autophagy detection kit 2.0 

A brighter and more photostable, no-transfection, quantitative assay for monitoring autophagy in live  cells
Flow Cytometry, Fluorescence microscopy, Fluorescent detection, HTS | Print as PDF
 
ENZ-KIT175-0050 50 tests 277.00 USD
 
ENZ-KIT175-0200 200 tests 586.00 USD
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CYTO-ID® Green long-term cell tracer kit 

Live cell fluorescent labeling over extended time periods with no apparent toxic effects
Flow Cytometry, Fluorescence microscopy, Fluorescent detection | Print as PDF
 
ENZ-51036-K025 1 Kit 260.00 USD
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CYTO-ID® Red long-term cell tracer kit  

Live  cell fluorescent labeling over extended time periods with no apparent toxic effects
Flow Cytometry, Fluorescence microscopy, Fluorescent detection | Print as PDF
 
ENZ-51037-K025 1 Kit 246.00 USD
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DNA damage ELISA kit 

Rapid DNA damage ELISA kit for cancer, apoptosis and oxidative stress.
ELISA, Colorimetric detection | Print as PDF
 
ADI-EKS-350 96 wells 931.00 USD
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LDH Cytotoxicity WST Assay 

Colorimetric kit for the determination of cytotoxicity through the measurement of lactate dehydrogenase activity
Colorimetric detection | Print as PDF
 
ENZ-KIT157-0500 500 tests 255.00 USD
 
ENZ-KIT157-2500 5x500 tests 979.00 USD
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NUCLEAR-ID® Blue/Green cell viability reagent 

Distinguishes between live vs dead cells in a single reagent.
≥93% (HPLC), Flow Cytometry, Fluorescence microscopy, Fluorescent detection | Print as PDF
 
ENZ-53004-C100 100 µl 316.00 USD
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NUCLEAR-ID® Blue/Red cell viability reagent (GFP-CERTIFIED®) 

Distinguishes between live vs dead cells in a single reagent.
≥93% (HPLC), Flow Cytometry, Fluorescence microscopy, Fluorescent detection | Print as PDF
 
ENZ-53005-C100 100 µl 304.00 USD
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NUCLEAR-ID® Red/Green cell viability reagent 

Distinguishes between live vs dead cells in a single reagent
≥93% (HPLC), Flow Cytometry, Fluorescence microscopy, Fluorescent detection | Print as PDF
 
ENZ-53006-C100 100 µl 310.00 USD
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p62 ELISA kit 

Highly validated, quantitative p62 ELISA kit available for autophagy research.
ELISA, Colorimetric detection | Print as PDF
 
ADI-900-212-0001 96 wells 677.00 USD
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PROTEOSTAT® Aggresome detection kit  

Robust, quantitative detection of aggresomes relevant to the study of neurodegenerative disease, liver disease and  toxicology
Flow Cytometry, Fluorescence microscopy, Fluorescent detection | Print as PDF
 
ENZ-51035-0025 25 tests 138.00 USD
 
ENZ-51035-K100 100 tests 398.00 USD
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