Development of Wnt pathway modulating drugs requires the ability to rapidly screen the effect of novel or repurposed compounds and biological therapeutics on the expression of Wnt-regulated genes, and confirmation of such effects by related changes in key signaling components. Together, the LEADING LIGHT™ Wnt Reporter Assay, LEADING LIGHT™ Sclerostin-LRP Screening System and SCREEN-WELL® Wnt Pathway Library facilitate high-throughput analysis of Wnt-related signaling.
The LEADING LIGHTt™ Wnt Reporter Assay Starter Kit is a high-sensitivity, luciferase-based assay for the analysis of canonical Wnt signaling. The assay uses a 3T3 mouse fibroblast cell line modified with a luciferase reporter gene under the control of Wnt-responsive promoters (TCF/LEF). The chemiluminescent readout is amenable to both 96-well and 384-well plate formats, facilitating high-throughput analysis of Wnt pathway activators and inhibitors.
Click on image to enlargeEnzo Life Sciences continues to expand its portfolio of Wnt Pathway assays with the NEW LEADING LIGHT™ Sclerostin-LRP Screening System. This simple, rapid biochemical binding assay format does not require cell lines or transfection reagents, making it a convenient platform for initial screening of inhibitors of the Wnt antagonist Sclerostin.
The SCREEN-WELL® Wnt Pathway Library offers a convenient solution for benchmarking compounds against a collection of 71 known Wnt modulators. The library is available in 100uL and 500uL formats (dissolved in DMSO) for use as a companion to the LEADING LIGHT™ Wnt Reporter Assay, or individually as a general screening tool for chemical genomics or Wnt assay.
| Wnt5a | Dkk | Axin | Porcupine | β-catenin | LRP5/6 |
| TCF | Dishevelled | GSK-3B | Adenylate Cyclase | COX-II | sFRP-1 |
Download our application note: Cell-Based Screening of Focused Bioactive Compound Libraries: Assessing Small Molecule Modulators of the Canonical Wnt Signaling and Autophagy-Lysosome Pathways.
LEADING LIGHT® Wnt Reporter Assay Starter Kit |
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| Chemiluminescent cell-based assay for discovering Wnt pathway modulators | ||||||
| FUNC, Chemiluminescence, HTS | Print as PDF | ||||||
| ENZ-61001-0001 | 96 wells | 1,248.00 USD |  |
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LEADING LIGHT® Wnt Reporter Cell Line |
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| Wnt reporter cell line for discovering Wnt pathway modulators | ||||||
| Print as PDF | ||||||
| ENZ-61002-0001 | 1 ml | 647.00 USD | |
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LEADING LIGHT® Wnt Cell Line Medium Pack |
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| For use with the LEADING LIGHT® Wnt Reporter Cell Line | ||||||
| Print as PDF | ||||||
| ENZ-60003-0001 | 1 Pack | 450.00 USD | |
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| Do you need bulk/larger quantities? | ||||||
LEADING LIGHT® Wnt Cell Line Assay Reagents |
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| For use with the LEADING LIGHT® Wnt Reporter Cell Line | ||||||
| Print as PDF | ||||||
| ENZ-60004-0001 | 1 Pack | 579.00 USD | |
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LEADING LIGHT® Sclerostin-LRP Interaction Screening System |
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| Novel Cell-free Sclerostin/LRP Binding Assay Ideal for Primary Inhibitor Screens | ||||||
| FUNC, Chemiluminescence, HTS | Print as PDF | ||||||
| ENZ-61003-0001 | 96 wells | 502.00 USD | |
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SCREEN-WELL® Wnt Pathway library |
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| Compounds are highly relevant; many are unique | ||||||
| HTS | Print as PDF | ||||||
| BML-2838-0100 | 1 Library | 100 µl/well | 1,882.00 USD | |
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| BML-2838-0500 | 1 Library | 500 µl/well | 4,507.00 USD | |
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BML-284 |
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| Wnt activator | ||||||
| 853220-52-7, ≥98% (HPLC) | Print as PDF | ||||||
| BML-WN105-0005 | 5 mg | 152.00 USD | |
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| BML-WN105-0025 | 25 mg | 608.00 USD | |
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QS11 |
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| Wnt pathway activator | ||||||
| 944328-88-5, ≥98% (HPLC) | Print as PDF | ||||||
| BML-WN117-0005 | 5 mg | 175.00 USD | |
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| BML-WN117-0025 | 25 mg | 733.00 USD | |
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