DNA Damage ELISA KIT

Rapidly monitor DNA destruction arising from cancer, apoptosis and oxidative stress using the DNA Damage ELISA kit
The DNA Damage ELISA (enzyme-linked immunosorbent assay) is a fast and sensitive immunoassay providing results in less than 2.5 hours. Quantitation of 8- hydroxy-2’-deoxyguanosine (8-OHdG) in urine, serum, and saliva samples is performed in a convenient 96-well plate format using a colorimetric substrate. 8-OHdG is a frequently-used critical biomarker of oxidative stress and carcinogenesis.

• Quantify levels < 1 ng/ml
• Validated in-house in a variety of sample matrices
• Tested in a variety of biofluids (urine, serum, and saliva)
• Convenient colorimetric 96-well plate format

Typical 8-OHdG Standard Curve

The standard curve has a range of 0.94 – 60 ng/mL.

COMET SCGE ASSAY KIT

Sensitive and versatile method for measuring single- and double-strand DNA breaks in individual cells.
Exposure of cells to oxidative and environmental stresses frequently results in the breakdown or oxidation of genomic DNA. Assays to evaluate the integrity of genomic DNA, or to assess the presence of oxidized DNA are frequently used as a means of verifying the onset of apoptosis or DNA damage. The COMET SCGE Assay measures DNA damage by fluorescently detecting the integrity of DNA liberated from cells embedded in low melting point agarose. Upon electrophoresis, fragmented DNA produces a characteristic “comet” shaped tail as small DNA fragments migrate in the gel more rapidly than in-tact genomic DNA.

The COMET SCGE Assay is a fast and simple electrophoresis method to detect and quantitate DNA fragmentation in cells associated with DNA damage and apoptosis. A unique nucleic acid stain provides improved sensitivity for DNA visualization compared to ethidium bromide.

• Comet Slides allow direct application of sample without pretreatment
• Shorter assay time allows for higher throughput sample analysis
• Hydrophobic barrier allows sample treatment with DNA repair enzymes
• Unique nucleic acid stain provides improved sensitivity for DNA visualization compared to ethidium bromide

Epigenetic control of skin development and regeneration is an active area of research. Diet and exposure to environmental stresses are two significant factors in determining a change in epigenetic patterns. As such personal care researchers are targeting epigenetic mechanisms as they formulate new products for the anti-aging market. Enzo offers a complete selection of active enzymes, antibodies and sensitivity detection assays for understanding epigenetic changes involving DNA methylation and post-translational modifications.