Simultaneously Monitor Hypoxic Status and ROS Formation

Quantitative Multiparametric Assay for Flow Cytometry

Glutathione (Total) Detection Kit

Glutathione Reductase reduces oxidized glutathione (GSSG) to reduced glutathione (GSH). The sulfhydryl group of GSH reacts with DTNB (5,5’-dithiobis-2-nitrobenzoic acid, Ellman’s reagent) to produce a yellow colored 5-thio-2-nitrobenzoic acid (TNB) that absorbs at 405 or 414 nm. The rate of TNB production is directly proportional to the concentration of glutathionein the sample.

• High-sensitivity enzymatic recycling reaction measures total, oxidized, or reduced glutathione
• Broad detection range: range (12.5 - 100 pmoles/well)
• Easy-to-use sample handling protocols for a wide variety of matrices
• Convenient colorimetric 96 well plate format

Protein carbolylation is a known irreversible result of oxidative damage that can lead to protein aggreggation and dysfunction.

Protein Carbonyl ELISA Kit

This convenient ELISA kit enables quantitative determination of carbonylated protein levels in plasma, other body fluids, cell and tissue extracts.

• High sensitivity
• Low background
• Amenable to high-throughput analysis
• Validated and widely cited for various sample matrices

Lipid peroxidation is a well-known example of oxidative damage, and lipid peroxides are prominent non-radical intermediates of lipid peroxidation. We offer a variety of assays and small molecules for screening the effects of anti-oxidants as potential inhibitors of this pathway.

ALDETECT™ (MDA-specific) Lipid Peroxidation Assay Kit

Designed to assay free malondialdehyde (MDA) or, after a hydrolysis step, total MDA (i.e., free and protein-bound Schiff base conjugates). Features include:

• Minimal interference from other lipid peroxidation products, such as 4-hydroxyalkenals
• Assess MDA levels from a variety of sample types including tissue and cell lysates
• Rapid results in less than 1.5 hours

Proteasome ELISA Kit

Examine the link between cell detoxification and proteasome concentrations with the only commercially available kit for proteasome quantification

• Quantify 20S proteasome concentrations in biological samples (serum, plasma, cell lysates, tissue extracts)
• Investigate effects of inhibitors and activiations on levels of circulating proteasome

p21 ELISA Kit

The p21 (human) ELISA kit is a colorimetric, immunometric enzyme immunoassay kit with results in 3 hours. Save time, money, and precious sample with fully quantitative results and increased sensitivity compared to Western blot analysis.

• Sensitive ELISA quantifies picogram levels compared to microgram levels in Western blot
• Excellent lot-to-lot reproducibility
• Easy-to-use liquid color-coded reagents reduce error

Protein homeostasis (proteostasis) is critical to the maintenance of cellular health. Enzo offers an industry-leading catalog of products for proteostasis research, including analysis of pathways controlling protein synthesis, folding, trafficking, aggregation, and degradation.

HSP70 High Sensitivity ELISA Kit

Only commercially available kit sensitive enough and specifically validated for quantifying HSP70 (HSP72) in serum and plasma samples.

• Sensitive ELISA quantifies as little as 90pg/ml HSP70 (HSP72)
• Fully validated for use with serum and plasma samples
• Specifically measures stress-induced HSP70 (HSP72) with negligible reactivity with other HSP70 family members (e.g. HSC70/HSP73, Grp78)
• High-throughput assay measures ~40 samples in duplicate in just 4.5 hours

Heat Shock Proteins

The family of heat shock proteins serve as chaperones to assist in folding, and are upregulated in conditions of cellular stress, helping to preserve cellular function during stress, and often directing cell fate should the stress persist. Enzo offers the definitive collection of more than 800 products for Heat Shock Protein and Cell Stress.