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Oxidative Stress

Determinaton of Antioxidant Capacity Through Oxidative Stress Biomarkers













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Reactive Oxygen Species

Multiplex, Real-time Analysis of Hypoxia and ROS in Live Cells

CYTO-ID® Hypoxia/Oxidative Stress Detection Kit

  • Sensitive hypoxia dye fluoresces red when converted by nitroreductases
  • Total ROS dye fluoresces green in presence of a variety of reactive oxygen species
  • Non-toxic, cell permeable dyes optimized to avoid artifactual fluorescence
  • Suitable for microscopic or flow cytometric analysis of adherent or suspension cells

Simultaneously Monitor Hypoxic Status and ROS Formation

Quantitative Multiparametric Assay for Flow Cytometry

 

Bright red fluorescence of the Hypoxia probe is observed following its conversion by cellular nitroreductases under hypoxic conditions such as those induced chemically by treatment with the hypoxia-mimetic desferrioxamine (DFO). The assay facilitates simultaneous quantification of hypoxic status (FL3/red) and presence of ROS (FL1/green) by flow cytometry.

ROS Detection Kits

ROS-ID® Hypoxia/Oxidative stress detection kit 

Specific detection system for simultaneous analysis of hypoxia and oxidative stress using microscopy and flow cytometry
Flow Cytometry, Fluorescence microscopy, Fluorescent detection, HTS | Print as PDF
 
ENZ-51042-0125 125 tests 113.00 USD
 
ENZ-51042-K500 500 tests 391.00 USD
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ROS-ID® ROS/RNS detection kit 

Monitor  reactive oxygen species and nitrogen species in live cells
Fluorescence microscopy | Print as PDF
 
ENZ-51001-200 1 Kit 297.00 USD
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ROS-ID® Total ROS/Superoxide detection kit 

Directly Monitor Global Levels  of Reactive Oxygen Species (ROS), and Specifically Superoxide
Flow Cytometry, Fluorescence microscopy, Fluorescent detection, HTS | Print as PDF
 
ENZ-51010 1 Kit 254.00 USD
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ROS-ID® Total ROS detection kit 

Flow Cytometry, Fluorescence microscopy, Fluorescent detection, HTS | Print as PDF
 
ENZ-51011 1 Kit 181.00 USD
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Probes for ROS

5(6)-CDCFDA (ultra pure) 

ROS Detector and pH indicator
127770-45-0, ≥98% (HPLC) | Print as PDF
 
ENZ-52103 100 mg 101.00 USD
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CFDA (ultra pure) 

ROS Detector and pH indicator
124387-19-5, ≥98% (HPLC) | Print as PDF
 
ENZ-52104 100 mg 103.00 USD
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Dihydrorhodamine 123 (ultra pure) 

Mitochondria dye
109244-58-8, ≥95% (HPLC) | Print as PDF
 
ENZ-52302 10 mg 168.00 USD
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ROS Inhibitors

APF 

Fluorescent probe for hROS detection
359010-70-1, ≥98% (HPLC) | Print as PDF
 
ALX-620-075-M001 1 mg 561.00 USD
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HPF 

Fluorescent probe for hROS detection
359010-69-8, ≥98% (HPLC) | Print as PDF
 
ALX-620-074-M001 1 mg 561.00 USD
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Glutathione

Glutathione (Total) Detection Kit

Glutathione Reductase reduces oxidized glutathione (GSSG) to reduced glutathione (GSH). The sulfhydryl group of GSH reacts with DTNB (5,5’-dithiobis-2-nitrobenzoic acid, Ellman’s reagent) to produce a yellow colored 5-thio-2-nitrobenzoic acid (TNB) that absorbs at 405 or 414 nm. The rate of TNB production is directly proportional to the concentration of glutathionein the sample.

  • High-sensitivity enzymatic recycling reaction measures total, oxidized, or reduced glutathione
  • Broad detection range: range (12.5 - 100 pmoles/well)
  • Easy-to-use sample handling protocols for a wide variety of matrices
  • Convenient colorimetric 96 well plate format

Measure Total Oxidized or Reduced Glutathione


Change in absorbance at 405 nm versus incubation time as a function of pmoles of GSSG/well.

 

Glutathione Assay Kits & Probes

Glutathione (GSSG/GSH), detection kit 

Rapid, ultra-sensitive kit for the quantification of reduced (GSH), oxidized (GSSG), and total glutathione for oxidative stress research.
Colorimetric detection | Print as PDF
 
ADI-900-160 4x96 wells 434.00 USD
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Glutathione peroxidase activity kit 

Colorimetric detection, Activity assay | Print as PDF
 
ADI-900-158 5x96 wells 633.00 USD
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Monobromobimane (ultra pure) 

Thiol dye
71418-44-5, ≥95% (HPLC) | Print as PDF
 
ENZ-52501 25 mg 103.00 USD
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Glutathione Antibodies

Glutathione monoclonal antibody (D8) 

ELISA, IP, WB | Print as PDF
 
ADI-SPA-542-E 100 µg 325.00 USD
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Glutathione S-Transferase monoclonal antibody (8C1-G4) 

Purified from hybridoma tissue culture supernatant., ELISA, IP, WB | Print as PDF
 
ADI-905-618-100 100 µg 306.00 USD
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Glutathione Peptides

L-Glutathione (reduced form) 

70-18-8, ≥99% | Print as PDF
 
ALX-157-002-G005 5 g 50.00 USD
 
ALX-157-002-G025 25 g 130.00 USD
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L-Glutathione (oxidized form) 

27025-41-8, ≥95.0% (HPLC) | Print as PDF
 
ALX-157-001-M250 250 mg 47.00 USD
 
ALX-157-001-G001 1 g 69.00 USD
 
ALX-157-001-G005 5 g 255.00 USD
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Glutathione Chemical Modulators

5,8,11-Eicosatriynoic acid 

Lipoxygenase inhibitor
13488-22-7, ≥98% (TLC) | Print as PDF
 
BML-ET003-0001 1 mg 85.00 USD
 
BML-ET003-0010 10 mg 608.00 USD
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Butein 

Tyrosine kinase inhibitor
487-52-5, ≥98% (HPLC) | Print as PDF
 
ALX-350-246-M010 10 mg 221.00 USD
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Buthionine sulfoximine 

γ-Glutamyl transpeptidase inhibitor
83730-53-4, ≥97% (TLC) | Print as PDF
 
BML-FR117-0500 500 mg 175.00 USD
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Cafestol 

GST activator
469-83-0, Isolated from the unsaponifiable fraction of petroleum ether extract of coffee beans., ≥98% (UHPLC) | Print as PDF
 
ALX-350-220-M050 50 mg 199.00 USD
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Carmustine 

DNA alkylating agent
154-93-8, ≥96% (Assay) | Print as PDF
 
ALX-400-037-M025 25 mg 87.00 USD
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Curcumin (high purity) 

Inhibitor of Lipoxygenase and COX
458-37-7, Isolated from turmeric (Curcuma longa)., ≥98% (HPLC) | Print as PDF
 
ALX-350-028-M010 10 mg 37.00 USD
 
ALX-350-028-M050 50 mg 91.00 USD
 
ALX-350-028-M250 250 mg 323.00 USD
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Ebselen 

Organoselenium compound. Antioxidant.
60940-34-3, ≥98% (HPLC) | Print as PDF
 
ALX-270-097-M005 5 mg 38.00 USD
 
ALX-270-097-M025 25 mg 77.00 USD
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Ethacrynic acid 

GST inhibitor
58-54-8, ≥98% | Print as PDF
 
BML-EI128-0001 1 g 38.00 USD
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Kahweol 

GST activator
6894-43-5, Natural product isolated from the unsaponifiable fraction of petroleum ether extract of coffee beans., ≥97% (UPLC) | Print as PDF
 
ALX-350-223-M010 10 mg 125.00 USD
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Prostaglandin A2 

CDK inhibitor
13345-50-1, Synthetic., ≥98% (TLC) | Print as PDF
 
BML-PG002-0010 10 mg 250.00 USD
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RSSR 

Supersensitive spin label
123557-49-3, ≥97% (HPLC, GLC) | Print as PDF
 
ALX-430-102-M025 25 mg 211.00 USD
 
ALX-430-102-M050 50 mg 375.00 USD
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S-(N-(3-Phenylpropyl)thiocarbamoyl)-L-cysteine 

GST activator
137915-13-0, ≥98% (TLC) | Print as PDF
 
BML-T114-0050 50 mg 58.00 USD
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Sedanolide 

GST activator
6415-59-4, Isolated from Apium graveolens L., ≥98% (UHPLC) | Print as PDF
 
ALX-350-229-M100 100 mg 167.00 USD
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Protein Carbonylation

Protein carbolylation is a known irreversible result of oxidative damage that can lead to protein aggreggation and dysfunction.

Protein Carbonyl ELISA Kit

This convenient ELISA kit enables quantitative determination of carbonylated protein levels in plasma, other body fluids, cell and tissue extracts.

  • High sensitivity
  • Low background
  • Amenable to high-throughput analysis
  • Validated and widely cited for various sample matrices


 

Lipid Peroxidation

Lipid peroxidation is a well-known example of oxidative damage, and lipid peroxides are prominent non-radical intermediates of lipid peroxidation. We offer a variety of assays and small molecules for screening the effects of anti-oxidants as potential inhibitors of this pathway.

ALDETECT™ (MDA-specific) Lipid Peroxidation Assay Kit

Designed to assay free malondialdehyde (MDA) or, after a hydrolysis step, total MDA (i.e., free and protein-bound Schiff base conjugates). Features include:

  • Minimal interference from other lipid peroxidation products, such as 4-hydroxyalkenals
  • Assess MDA levels from a variety of sample types including tissue and cell lysates
  • Rapid results in less than 1.5 hours


 

Lipid Peroxidation Assays

ALDetect™ Lipid Peroxidation assay kit 

Colorimetric detection | Print as PDF
 
BML-AK170-0001 100 tests 639.00 USD
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ALDetect™ (MDA-specific) Lipid Peroxidation assay kit 

Colorimetric detection | Print as PDF
 
BML-AK171-0001 25 tests 705.00 USD
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OXI-TEK TBARS assay kit 

Fluorescence microscopy | Print as PDF
 
ALX-850-287-KI01 160 tests 681.00 USD
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Biochemical Modulators of Oxidative Stress

(-)-Epigallocatechin gallate 

Inhibitor of NOS, telomerase and Dnmt
989-51-5, Isolated from green tea., ≥98% (HPLC) | Print as PDF
 
ALX-270-263-M010 10 mg 37.00 USD
 
ALX-270-263-M050 50 mg 71.00 USD
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(Z)-4-Hydroxytamoxifen 

Tamoxifen metabolite
68047-06-3, 65213-48-1, ≥98% (UHPLC) | Print as PDF
 
ALX-550-361-M001 1 mg 57.00 USD
 
ALX-550-361-M005 5 mg 197.00 USD
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Auraptene 

Anti-inflammatory and chemopreventive
495-02-3, Isolated from citrus fruit., ≥95% (UPLC) | Print as PDF
 
ALX-350-361-M025 25 mg 91.00 USD
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Bakuchiol 

PTP1B inhibitor
10309-37-2, Isolated from plant Psoralea corylifolia., ≥97% (HPLC) | Print as PDF
 
ALX-350-144-M001 1 mg 47.00 USD
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Caffeic acid phenylethyl ester 

HIV-1 integrase inhibitor
104594-70-9, Synthetic., ≥97% (HPLC) | Print as PDF
 
ALX-270-244-M010 10 mg 36.00 USD
 
ALX-270-244-M050 50 mg 79.00 USD
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Carazostatin 

Free radical scavenger
126168-32-9, Isolated from Streptomyces chrestomyceticus. | Print as PDF
 
ALX-350-253-C100 100 µg 50.00 USD
 
ALX-350-253-M001 1 mg 112.00 USD
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Carnosic acid 

PPARγ activator. Antioxidant.
3650-09-7, Isolated from Rosmarinus officinalis., ≥95% (HPLC) | Print as PDF
 
ALX-270-264-M010 10 mg 61.00 USD
 
ALX-270-264-M050 50 mg 176.00 USD
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Celastrol 

Anti-inflammatory and immunosuppressive
34157-83-0, Isolated from the root of Tripterygium sp., ≥98% (HPLC) | Print as PDF
 
ALX-350-332-M005 5 mg 86.00 USD
 
ALX-350-332-M025 25 mg 312.00 USD
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Cinnamtannin B-1 

Potent antioxidant
88082-60-4, Isolated from Laurus nobilis L., ≥95% (HPLC) | Print as PDF
 
ALX-350-365-M005 5 mg 276.00 USD
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Idebenone 

Quinone antioxidant
58186-27-9, ≥98% (HPLC) | Print as PDF
 
BML-FR114-0020 20 mg 90.00 USD
 
BML-FR114-0100 100 mg 369.00 USD
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Morin 

Cell cycle inhibitor
654055-01-3, ≥85% (HPLC) | Print as PDF
 
ALX-385-016-G001 1 g 37.00 USD
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Ochratoxin A 

Mycotoxin. Nephrotoxic.
303-47-9, Isolated from Aspergillus ochraceus., ≥98% (HPLC, TLC) | Print as PDF
 
ALX-630-089-M001 1 mg 65.00 USD
 
ALX-630-089-M005 5 mg 244.00 USD
 
ALX-630-089-M025 25 mg 886.00 USD
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Rosmarinic acid 

537-15-5, 20283-92-5, Isolated from Rosmarinus officinalis., ≥97% (HPLC) | Print as PDF
 
ALX-270-253-M010 10 mg 36.00 USD
 
ALX-270-253-M050 50 mg 101.00 USD
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Trolox® 

Cell permeable vitamin E derivative
53188-07-1; 56305-04-5, ≥96.5% (Titration (T) NaOH 0.1M) | Print as PDF
 
ALX-270-267-M100 100 mg 37.00 USD
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U-74389G 

Blocks lipid peroxidation
153190-29-5, ≥95% (HPLC) | Print as PDF
 
BML-FR100-0100 100 mg 125.00 USD
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Cell Detoxification

Proteasome ELISA Kit

Examine the link between cell detoxification and proteasome concentrations with the only commercially available kit for proteasome quantification

  • Quantify 20S proteasome concentrations in biological samples (serum, plasma, cell lysates, tissue extracts)
  • Investigate effects of inhibitors and activiations on levels of circulating proteasome


Quantify Circulating Levels of 20S Proteasome

 

Related Products

Proteasome 20S (human), (purified) 

High integrity preparation for use in proteasome research.
Isolated and purified from human erythrocytes. | Print as PDF
 
BML-PW8720-0050 50 µg 210.00 USD
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Immunoproteasome 20S (human), (purified) 

Isolated from human spleen. | Print as PDF
 
BML-PW9645-0050 50 µg 496.00 USD
Do you need bulk/larger quantities?
 

Proteasome 26S (human), (purified) 

High integrity preparation for use in proteasome research.
Isolated from human erythrocytes. Consists of a high purity mixture of ‘26S’ proteasomes singly (26S) and doubly (30S) capped with 19S regulatory subunit complexes in the ratio of 40% single cap : 60% double capped at the time of preparation. | Print as PDF
 
BML-PW9310-0050 50 µg 390.00 USD
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Cell Senescence

p21 ELISA Kit

The p21 (human) ELISA kit is a colorimetric, immunometric enzyme immunoassay kit with results in 3 hours. Save time, money, and precious sample with fully quantitative results and increased sensitivity compared to Western blot analysis.

  • Sensitive ELISA quantifies picogram levels compared to microgram levels in Western blot
  • Excellent lot-to-lot reproducibility
  • Easy-to-use liquid color-coded reagents reduce error


Monitor Levels of P21, a Regulator of Senescence


Levels of P21 in control (DMSO or Apicidin treated HeLa cell lysates as determined using P21 ELISA kit

 

Proteostasis

Protein homeostasis (proteostasis) is critical to the maintenance of cellular health. Enzo offers an industry-leading catalog of products for proteostasis research, including analysis of pathways controlling protein synthesis, folding, trafficking, aggregation, and degradation.

 

HSP70 High Sensitivity ELISA Kit

Only commercially available kit sensitive enough and specifically validated for quantifying HSP70 (HSP72) in serum and plasma samples.

  • Sensitive ELISA quantifies as little as 90pg/ml HSP70 (HSP72)
  • Fully validated for use with serum and plasma samples
  • Specifically measures stress-induced HSP70 (HSP72) with negligible reactivity with other HSP70 family members (e.g. HSC70/HSP73, Grp78)
  • High-throughput assay measures ~40 samples in duplicate in just 4.5 hours

Delivers Sensitivity to Quantify HSP70 in Serum & Plasma


Typical standard curve for HSP70 High Sensitivity ELISA Kit

 

Heat Shock Proteins

The family of heat shock proteins serve as chaperones to assist in folding, and are upregulated in conditions of cellular stress, helping to preserve cellular function during stress, and often directing cell fate should the stress persist. Enzo offers the definitive collection of more than 800 products for Heat Shock Protein and Cell Stress.


 

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