A high-sensitivity colorimetric assay for the determination of the number of viable cells,using WST8, in cell proliferation and cytotoxicity assays. This critical assay can be used to screen modulators of cell proliferation that affect the appearance of wrinkles and other signs of aging.
More sensitive than MTT, XTT, MTS or WST-1
Ready-to-use solution can be added directly to the cell media
No organic solvents or isotopes required
No harvesting, washing, or solubilization steps
Amenable to high-throughput screening
High-sensitivity Cell Proliferation Assay
CTLL-2 cells were incubated with various concentrations of IL-2 for 72 hours. CCK-8 solution was added to each well and the absorbance at 450nm was measured. IL-2 exposure resulted in increased,corrleating to an increase in cell proliferation.
Our stable NUCLEAR-ID® Red DNA stain crosses the membrane of live cells and specifically targets dsDNA. The dye can be used for various applications including nucleated cell gating in flow cytometry, cell cycle ploide analysis, and cytoplasm segmentation in high content screening (HCS).
Brighter, photostable red dye yields improved sensitivity
Highly cell permeable dye minimizes cell density optimization
Lower cost per test than comparable dyes
Suitable for multiplexing with green fluorophores, including GFP and FITC
Available as a complete kit with controls or as a stand-alone dye
Growth Factors
Growth factors are frequently used in cosmetics to reverse the signs and symptoms of instrinsic skin aging due to the aging process and extrinsic aging mediated by environmental factors.
A critical factor in angiogenesis, wound healing and embryonic development.
Carrier-free
High purity, ≥97%
Activity tested in cell proliferation assay
3T3 cells were cultured with 0 to 10ng/ml human FGF basic. Cell proliferation was measured after 44 hours and the linear portion of the curve was us used to calculate the ED50. The ED50 for this lot of human FGF basic was 0.042-0.063ng/ml. The typical expected range is less than 1ng/ml.
Produced in E. coli. Non-glycosylated protein, containing 54 amino acids, with a molecular weight of 6.3 kDa., ≥95% (HPLC, Reducing and Non-reducing SDS-PAGE, UV spectroscopy at 280 nm) | Print as PDF
Produced in baculovirus. The 46kDa cytoplasmic domain of the ß-subunit of human IGF-1 receptor kinase (IGF-1RK ) (aa 929-1337) is fused to GST. | Print as PDF
Produced in E. coli. Non-glycosylated, disulfide-linked homodimer, containing two 112 amino acid chains., ≥98% (Reducing and Non-reducing SDS-PAGE) | Print as PDF