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Cell Proliferation

Convenient Assays, Sensitive Results





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Cell Proliferation Assays

Cell Counting Kit-8

A high-sensitivity colorimetric assay for the determination of the number of viable cells,using WST8, in cell proliferation and cytotoxicity assays. This critical assay can be used to screen modulators of cell proliferation that affect the appearance of wrinkles and other signs of aging.

  • More sensitive than MTT, XTT, MTS or WST-1
  • Ready-to-use solution can be added directly to the cell media
  • No organic solvents or isotopes required
  • No harvesting, washing, or solubilization steps
  • Amenable to high-throughput screening

High-sensitivity Cell Proliferation Assay


CTLL-2 cells were incubated with various concentrations of IL-2 for 72 hours. CCK-8 solution was added to each well and the absorbance at 450nm was measured. IL-2 exposure resulted in increased,corrleating to an increase in cell proliferation.

 

Proliferation Related ELISA Kits

Erk1/2 ELISA kit 

ELISA, Colorimetric detection | Print as PDF
 
ADI-900-152 96 wells 392.00 USD
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[pThr202/Tyr204]Erk1/2 ELISA kit 

Sensitive ELISA kit for this key cell signaling molecule for kinase, proliferation, apoptosis, and differentiation research.
ELISA, Colorimetric detection | Print as PDF
 
ADI-900-098A-0001 96 wells 673.00 USD
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IGF-1 (human), ELISA kit 

Highly sensitive ELISA, measuring as little as 34.2 pg/ml of IGF-1 in just 4 hours.
ELISA, Colorimetric detection | Print as PDF
 
ADI-900-150 96 wells 571.00 USD
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TGF-β1 ELISA kit 

ELISA, Colorimetric detection | Print as PDF
 
ADI-900-155 96 wells 516.00 USD
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Cell Proliferation Inhibitors

Tyrphostin AG 1024 

IGF-1 inhibitor
65678-07-1, ≥98% (HPLC (UV)) | Print as PDF
 
ALX-270-217-M001 1 mg 86.00 USD
 
ALX-270-217-M005 5 mg 316.00 USD
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Reveromycin D 

Cell cycle inhibitor
144860-70-8, Isolated from Streptomyces sp. MST-AS5344., ≥95% (HPLC) | Print as PDF
 
ALX-380-219-MC25 0.25 mg 251.00 USD
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Cell Cycle Analysis

The Brightest, Most Sensitive Cell Cycle Analysis Dye for Live Cells

NUCLEAR-ID® Red Cell Cycle Kit

Our stable NUCLEAR-ID® Red DNA stain crosses the membrane of live cells and specifically targets dsDNA. The dye can be used for various applications including nucleated cell gating in flow cytometry, cell cycle ploide analysis, and cytoplasm segmentation in high content screening (HCS).

  • Brighter, photostable red dye yields improved sensitivity
  • Highly cell permeable dye minimizes cell density optimization
  • Lower cost per test than comparable dyes
  • Suitable for multiplexing with green fluorophores, including GFP and FITC
  • Available as a complete kit with controls or as a stand-alone dye

 

Growth Factors

Growth factors are frequently used in cosmetics to reverse the signs and symptoms of instrinsic skin aging due to the aging process and extrinsic aging mediated by environmental factors.

 

FGF-basic 147 (human)

A critical factor in angiogenesis, wound healing and embryonic development.

  • Carrier-free
  • High purity, ≥97%
  • Activity tested in cell proliferation assay



3T3 cells were cultured with 0 to 10ng/ml human FGF basic. Cell proliferation was measured after 44 hours and the linear portion of the curve was us used to calculate the ED50. The ED50 for this lot of human FGF basic was 0.042-0.063ng/ml. The typical expected range is less than 1ng/ml.

 

Antibodies

Fibroblast growth factor 1 monoclonal antibody (4D2) 

WB | Print as PDF
 
ADI-905-883-100 100 µl 418.00 USD
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Insulin-like growth factor-1 receptor monoclonal antibody (1-2) 

Purified from mouse ascites., IP, WB | Print as PDF
 
BML-SA369-0100 100 µg 526.00 USD
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Active Proteins

Epidermal growth factor (human), (recombinant) 

Produced in E. coli., ≥98% (SDS-PAGE; RP-HPLC), ELISA, WB, in vitro Assay | Print as PDF
 
ADI-908-060-100 100 µg 197.00 USD
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Epidermal growth factor (rat), (recombinant) 

Produced in E. coli. Non-glycosylated protein, containing 54 amino acids, with a molecular weight of 6.3 kDa., ≥95% (HPLC, Reducing and Non-reducing SDS-PAGE, UV spectroscopy at 280 nm) | Print as PDF
 
ALX-201-812-0100 100 µg 251.00 USD
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FGF-basic 147 (human), (recombinant) 

Produced in E. coli. Non-glycosylated protein, containing 147 amino acids., ≥95% (Reducing and Non-reducing SDS-PAGE, UV spectroscopy at 280 nm) | Print as PDF
 
ALX-201-822-0010 10 µg 149.00 USD
 
ALX-201-822-0050 50 µg 249.00 USD
 
ALX-201-822-0100 100 µg 700.00 USD
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FGF-basic 154 (human), (recombinant) 

Produced in E. coli. Non-glycosylated protein, containing 154 amino acids., ≥95% (Reducing and Non-reducing SDS-PAGE) | Print as PDF
 
ALX-201-823-0010 10 µg 149.00 USD
 
ALX-201-823-0050 50 µg 221.00 USD
 
ALX-201-823-0100 100 µg 643.00 USD
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FGF basic (mouse), (recombinant) 

Produced in E. coli. Non-glycosylated protein, containing 145 amino acids., ≥95% (Reducing and Non-reducing SDS-PAGE) | Print as PDF
 
ALX-201-824-0010 10 µg 149.00 USD
 
ALX-201-824-0050 50 µg 251.00 USD
 
ALX-201-824-0100 100 µg 700.00 USD
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IGF-I (human), (recombinant) 

Produced in E. coli., ≥95% (SDS and HPLC), ELISA, WB, Activity assay | Print as PDF
 
ADI-908-059-0100 100 µg 140.00 USD
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Insulin-like growth factor-1 receptor (human), (recombinant) (GST-tag) 

Produced in baculovirus. The 46kDa cytoplasmic domain of the ß-subunit of human IGF-1 receptor kinase (IGF-1RK ) (aa 929-1337) is fused to GST. | Print as PDF
 
BML-SE232-0020 20 µg 382.00 USD
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TGF-β3 (mouse), (recombinant) 

Produced in E. coli. Non-glycosylated, disulfide-linked homodimer, containing two 112 amino acid chains., ≥98% (Reducing and Non-reducing SDS-PAGE) | Print as PDF
 
ALX-201-817-0010 10 µg 231.00 USD
 
ALX-201-817-0100 100 µg 774.00 USD
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