Tools to Streamline Your NGS Workflow

Next generation sequencing (NGS) is a method of high-throughput sequencing in which millions of DNA strands can be sequenced in parallel. Many researchers use NGS as an important tool to gain insight into the detailed and comprehensive genetic and epigenetic modifications of genomes from many different cell types. Choose from our comprehensive mix of kits to extract and prepare your library sample for NGS analysis.

Whether you are preparing samples for ChIP-seq, whole genome sequencing, or Bisuilfite-sequencing our kits are easy, efficient, and convenient.

Enable Proven, Consistent array CGH

CYTAG® CGH Labeling Kit

Array-based comparative genomic hybridization (aCGH) is a powerful tool for detecting gene copy number gains and losses associated with chromosome abnormalities. Detecting chromosomal aberrations by array CGH is faster, more robust and provides superior results over other technologies such as FISH and G-banding karyotyping, thus providing a greater understanding of the role of chromosomal changes in genetic diseases and cancers. Enzo superior labeling technology yields the industry’s most consistent CGH results.

CYTAG® TotalCGH Labeling Kit

For a complete solution to prepare DNA for CGH+SNP analysis, Enzo offers the CYTAG® TotalCGH Labeling Kit. Included in the kit are restriction enzymes AluI and RsaI for DNA digestion, the CYTAG® CGH Labeling kit for superior labeling, and PCR & Gel Clean-up columns for purifying labeled DNA.

CYTAG® SuperCGH Labeling Kit

Enzo is now offering CYTAG® SuperCGH Labeling Kits for producing high quality oligo array data using as little as 50 ng of genomic DNA, without a need for pre-amplification for precious low input samples. Validated for a wide range of low input samples including blood, amniotic fluid, postnatal and oncology samples, our CYTAG® SuperCGH Labeling Kit is your solution for labeling difficult samples.

Gene Expression and Transcript Analysis

BIOARRAY® Amplification & Labeling Kits

Enzo’s BIOARRAY® RNA Amplification & Labeling kits offer labeling solutions for biotin-based gene expression microarrays. Due to greater biotin incorporation and brighter signal, BIOARRAY® RNA labeling is the best choice for routine GeneChip® analysis.

Analyze Limiting Quantities of Total RNA

Don’t let the quantity of starting material limit your assay. Our BIOARRAY® Low Input RNA Amplification and Biotin Labeling System generates sufficient biotin labeled aRNA from as little as 20ng of total input RNA.

AMPIPROBE® Nucleic Acid Detection

AMPIPROBE® incorporates Enzo’s proprietary primer design in a unique qPCR platform that enables sensitive detection of nucleic acids. AMPIPROBE® assay kits, a low-cost alternative in a price-sensitive market, are designed for clinically relevant targets. Enzo’s landmark AMPIPROBE® assay, the AMPIPROBE® HCV assay kit, is a sensitive qPCR assay to measure Hepatitis C viral load.

AMPIGENE® PCR and qPCR Solutions

Enzo offers solutions for both traditional PCR and qPCR. Our AMPIGENE® Taq Mixes and RT-PCR Kit are optimized for standard PCR experiments and come in a variety of flexible formats to enable easy PCR and RT-PCR. In addition, we offer AMPIGENE® qPCR Kits and Mixes for both dye-based and probe-based real-time PCR. Enhanced buffers with stabilizers prevent the formation of primers/dimers to ensure sensitive and specific results for your qPCR and RT-qPCR applications.

CYGREEN® Nucleic Acid Dye

CYGREEN® Nucleic acid dye can be used for gel staining, higher sensitivity qPCR, and melt curve analysis. It has excitation/emission spectra similar to commonly used qPCR dyes and can be substituted in to increase sensitivity and reduce cost.

PCR & Gel clean-up columns

PCR & Gel clean-up columns use silica membrane technology in a mini spin column format to deliver easy and efficient DNA purification from PCR products, agarose gels, and labeling reactions.

• High recoveries for fragments down to 50 bp
• Small elution volume down to 15 µl
• Binding buffer with pH indicator
• Validated for use with CGH labeling kit