Intracellular calcium mobilization is critical to maintaining cellular depolarization during ventricular contraction. Enzo FluoForte dyes are optimized for high-throughput detection of intracellular calcium in living cells such as cardiomyocytes.
Superior Detection of Calcium Mobilization Compared to the Competition
FLUOFORTE exhibits significantly brighter fluorescence intensity than Fluo-3 AM and Fluo-4 AM. Evaluation of relative dye fluorescence. U2OS cells were seeded overnight, growth medium was removed, and cells were incubated with 100 μl of FLUOFORTE® AM, Fluo-3 AM, or Fluo-4 AM in HHBS at 37 °C, 5% CO2 incubator for 1 hour. The cells were washed twice with 200 μl HHBS, and ATP (20 μL/well) was added to achieve concentrations of 200 nM with dye efflux inhibitor. Cells were then immediately imaged with a fluorescence microscope (Olympus IX71) using FITC channel.
FLUOFORTE® Calcium assay kit |
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| A Brighter, More Robust Fluorescent Assay for Calcium Mobilization | ||||||
| Flow Cytometry, Fluorescence microscopy, Fluorescent detection, HTS | Print as PDF | ||||||
| ENZ-51016 | 100x96 tests | 3,206.00 USD |  |
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| Do you need bulk/larger quantities? | ||||||
FLUOFORTE® Calcium assay kit |
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| A Brighter, More Robust Fluorescent Assay for Calcium Mobilization | ||||||
| Flow Cytometry, Fluorescence microscopy, Fluorescent detection, HTS | Print as PDF | ||||||
| ENZ-51017 | 10x96 tests | 434.00 USD | |
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| Do you need bulk/larger quantities? | ||||||
GFP-CERTIFIED® FLUOFORTE® Calcium assay kit |
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| A brighter, more robust and GFP-compatible (orange dye) fluorescent assay for calcium mobilization | ||||||
| Flow Cytometry, Fluorescence microscopy, Fluorescent detection, HTS | Print as PDF | ||||||
| ENZ-51020-KP010 | 10x96 wells | 582.00 USD | |
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| ENZ-51020-KP100 | 100x96 wells | 4,090.00 USD | |
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| Do you need bulk/larger quantities? | ||||||
FLUOFORTE® Reagent |
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| Calcium dye | ||||||
| ≥95% (HPLC), Flow Cytometry, Fluorescence microscopy, Fluorescent detection, HTS | Print as PDF | ||||||
| ENZ-52014 | 5x50 µg | 313.00 USD | |
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| Do you need bulk/larger quantities? | ||||||
FLUOFORTE® Reagent |
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| Calcium dye | ||||||
| ≥95% (HPLC), Flow Cytometry, Fluorescence microscopy, Fluorescent detection, HTS | Print as PDF | ||||||
| ENZ-52015 | 1 mg | 387.00 USD | |
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| Do you need bulk/larger quantities? | ||||||
GFP-CERTIFIED® FLUOFORTE® Reagent |
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| Calcium dye | ||||||
| ≥95% (HPLC), Flow Cytometry, Fluorescence microscopy, Fluorescent detection, HTS | Print as PDF | ||||||
| ENZ-52016-5C50 | 5x50 µg | 332.00 USD | |
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| ENZ-52016-M001 | 1 mg | 402.00 USD | |
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| Do you need bulk/larger quantities? | ||||||
