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Monitor Calcium Mobilization

A Brighter, More Robust Fluorescent Assay for Calcium Mobilization

FLUOFORTE® Calcium Assay Kits

Intracellular calcium mobilization is critical to maintaining cellular depolarization during ventricular contraction. Enzo FluoForte dyes are optimized for high-throughput detection of intracellular calcium in living cells such as cardiomyocytes.

  • Dye optimized for superior cell-permeability and retention
  • Economical alternative developed for use with conventional dual-dispensing microplate readers
  • Provides EC50 values comparable to Fluo-4 and Calcium 4
  • Optional red dye enables multiplexing with green fluorophores

Superior Detection of Calcium Mobilization Compared to the Competition

FLUOFORTE exhibits significantly brighter fluorescence intensity than Fluo-3 AM and Fluo-4 AM. Evaluation of relative dye fluorescence. U2OS cells were seeded overnight, growth medium was removed, and cells were incubated with 100 μl of FLUOFORTE® AM, Fluo-3 AM, or Fluo-4 AM in HHBS at 37 °C, 5% CO2 incubator for 1 hour. The cells were washed twice with 200 μl HHBS, and ATP (20 μL/well) was added to achieve concentrations of 200 nM with dye efflux inhibitor. Cells were then immediately imaged with a fluorescence microscope (Olympus IX71) using FITC channel.

 

FLUOFORTE® Calcium assay kit  

A Brighter, More Robust Fluorescent Assay for Calcium Mobilization
Flow Cytometry, Fluorescence microscopy, Fluorescent detection, HTS | Print as PDF
 
ENZ-51016 100x96 tests 2,639.00 USD
Do you need bulk/larger quantities?
 

FLUOFORTE® Calcium assay kit  

A Brighter, More Robust Fluorescent Assay for Calcium Mobilization
Flow Cytometry, Fluorescence microscopy, Fluorescent detection, HTS | Print as PDF
 
ENZ-51017 10x96 tests 365.00 USD
Do you need bulk/larger quantities?
 

GFP-CERTIFIED® FLUOFORTE® Calcium assay kit 

A brighter, more robust and GFP-compatible (orange dye) fluorescent assay for calcium mobilization
Flow Cytometry, Fluorescence microscopy, Fluorescent detection, HTS | Print as PDF
 
ENZ-51020-KP010 10x96 wells 490.00 USD
 
ENZ-51020-KP100 100x96 wells 3,432.00 USD
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FLUOFORTE® Reagent 

Calcium dye
≥95% (HPLC), Flow Cytometry, Fluorescence microscopy, Fluorescent detection, HTS | Print as PDF
 
ENZ-52014 5x50 µg 263.00 USD
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FLUOFORTE® Reagent 

Calcium dye
≥95% (HPLC), Flow Cytometry, Fluorescence microscopy, Fluorescent detection, HTS | Print as PDF
 
ENZ-52015 1 mg 325.00 USD
Do you need bulk/larger quantities?
 

GFP-CERTIFIED® FLUOFORTE® Reagent 

Calcium dye
≥95% (HPLC), Flow Cytometry, Fluorescence microscopy, Fluorescent detection, HTS | Print as PDF
 
ENZ-52016-5C50 5x50 µg 275.00 USD
 
ENZ-52016-M001 1 mg 332.00 USD
Do you need bulk/larger quantities?
 

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