- Sensitive - detect as little as 19 pg/ml MPO
- Reliable - excellent reproducibility and parallelism
- Convenient - ready-to-use liquid color-coded reagents reduce error and save time in the lab
- Higher throughput - results for up to 39 samples in duplicate in just 3 hours
The Myeloperoxidase (human), EIA kit is a colorimetric immunometric enzyme immunoassay kit with results in 3 hours. This sensitive kit lets you quantitate as little as 19 pg/mL MPO in a wide variety of sample matrices that have been validated in-house. Convenient ready-to-use liquid color-coded reagents reduce error and save time in the lab.
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Product Details
| Alternative Name: | MPO |
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| Sensitivity: | Assay Buffer 13: 0.028ng/mL
Assay Buffer 31: 0.019ng/mL
(range 0.195 - 12.5 ng/ml) |
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| Assay Time: | 3 hours |
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| Applications: | ELISA, Colorimetric detection
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| Application Notes: | For the quantitative determination of human Myeloperoxidase of culture supernatants, nasal lavage, Lithium-heparin plasma, sputum supernatant, and urine. |
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| Wavelength: | 450 nm |
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| Species reactivity: | Human
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| Crossreactivity: | Human Myeloperoxidase (100%), human Erythropoietin (1.1%), bovine Lactoperoxidase (0.40%), human Elastase (0.20%), human Lactoferrin (0.20%), sheep COX-2 (0.10%) and <0.10%: human Thyroid Peroxidase, human Eosinophil-derived Neurotoxin |
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| Shipping: | Blue Ice Not Frozen |
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| Long Term Storage: | +4°C |
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| Contents: | Microtiter plate, Conjugate, Antibody, Assay buffer 13, Assay buffer 31 concentrate, Wash buffer concentrate, Standard, TMB Substrate, Stop solution 2 |
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| Scientific Background: | Myeloperoxidase (MPO) is synthesized as a single chain precursor, which is then processed into a tetramer consisting of 2 light chains and 2 heavy chains. In response to microbial invasion, MPO is released from the cytoplasmic granules of neutrophils into the extracellular space and phagosome, where it generates hypohalous acids that have a strong microbicidal effect. Myeloperoxidase can be used as a marker of airway inflammation caused either by disease, such as asthma, or by environmental irritants. |
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| UniProt ID: | P05164 |
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| Regulatory Status: | RUO - Research Use Only |
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| Compatibility: | This product is compatible with the Absorbance 96 Plate Reader.
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Product Literature References
Circulating Neutrophils of Nonalcoholic Steatohepatitis Patients Show an Activated Phenotype and Suppress T Lymphocytes Activity: L. Antonucci, et al.; J. Immunol. Res.
2020, 4570219 (2020),
Abstract;
Full Text
The AIM2 inflammasome is a central regulator of intestinal homeostasis through the IL-18/IL-22/STAT3 pathway: R.A. Ratsimandresy, et al.; Cell. Mol. Immunol.
14, 127 (2017),
Application(s): MPO activity, colon homogenates,
Abstract;
Full Text
Usefulness of Plasma Matrix Metalloproteinase-3 (MMP-3) Levels to Predict Myocardial Infarction in Men with and without Acute Coronary Syndrome: E. Cavusoglu, et al.; Am. J. Cardiol.
117, 881 (2016),
Application(s): ELISA measuring plasma levels of myeloperoxidase,
Abstract;
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