Qualitative Measurement of SARS-CoV-2 Spike and Nucleocapsid IgG Antibodies in Serum Samples
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Cathy Olsen, PhD | Sr. Applications Scientist | Molecular Devices
Heather Mary Brown, PhD | Application Scientist | Enzo Life Sciences
Featured Product: SARS-CoV-2 Spike IgG ELISA Kit (RUO), SARS-CoV-2 Nucleocapsid IgG ELISA Kit (RUO)
INTRODUCTION
Rapid, accurate, and frequent molecular testing is of critical importance in navigating the SARS-CoV-2 (COVID-19) pandemic. To advance our understanding of the relationship between infection and immunity, it is critical to test not only for infection, but also for antibodies. The fundamental challenge is to identify and implement sensitive, consistent, and accessible testing methods that fulfill these requirements, all at an affordable price. Quantitative RT-PCR testing for detecting viral mRNA is currently the most accurate test on the market for identifying an existing infection. However, it does not shed light on the immune response. Given that significant emphasis has been placed on developing testing methods to identify disease, advancements in assay development for antibody testing have remained insufficient. The antibody tests currently on the market return many false positive results and therefore are not a reliable method for understanding the nuances of the immune response to SARS-CoV-21. Thus, a reliable, high-throughput method for investigating COVID-19 immunity and antibody response is an unmet need.
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