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Qualitative Measurement of SARS-CoV-2 Spike and Nucleocapsid IgG Antibodies in Serum Samples

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Cathy Olsen, PhD | Sr. Applications Scientist | Molecular Devices

Heather Mary Brown, PhD | Application Scientist | Enzo Life Sciences

Featured Product: SARS-CoV-2 Spike IgG ELISA Kit (RUO), SARS-CoV-2 Nucleocapsid IgG ELISA Kit (RUO)


INTRODUCTION

Rapid, accurate, and frequent molecular testing is of critical importance in navigating the SARS-CoV-2 (COVID-19) pandemic. To advance our understanding of the relationship between infection and immunity, it is critical to test not only for infection, but also for antibodies. The fundamental challenge is to identify and implement sensitive, consistent, and accessible testing methods that fulfill these requirements, all at an affordable price. Quantitative RT-PCR testing for detecting viral mRNA is currently the most accurate test on the market for identifying an existing infection. However, it does not shed light on the immune response. Given that significant emphasis has been placed on developing testing methods to identify disease, advancements in assay development for antibody testing have remained insufficient. The antibody tests currently on the market return many false positive results and therefore are not a reliable method for understanding the nuances of the immune response to SARS-CoV-21. Thus, a reliable, high-throughput method for investigating COVID-19 immunity and antibody response is an unmet need.

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Enzo offers research kits, biochemicals and biologicals backed by over 40 years of expertise. As Scientists Enabling Scientists™, we realize the value in providing relevant information to our customers working in the fields of life sciences, drug development and clinical research. We are happy to provide simple but useful tips for improving daily tasks as well as the overall quality of your research. With this in mind, here is an e-book that answers important questions such as:

  • What are the Differences Between ELISA Assay Types?
  • 10 Tips for Successful ELISA Assays
  • In-depth Insights on ELISA:
    • %CV in ELISA: How to reduce them and why they’re important
    • Dilutional linearity, parallelism, spike-and-recovery in ELISA: How to QC your results?
    • How to validate sample dilutions and achieve linearity in new sample types

 

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