Jessica Antos¹, Colten Wimmer², Hartmut Pohl^3
1Enzo Life Sciences, Ann Arbor, Michigan
²Byonoy GmbH, Hamburg, Germany
³Enzo Life Sciences, Lausen, Switzerland

Featured Product: Absorbance 96 Plate Reader

INTRODUCTION

Since its introduction, Enzyme Linked Immunosorbent Assay (ELISA) has become a widespread and highly trusted tool to quantify a wide array of diverse analytes from small molecules to quaternary protein complexes. It has become a workhorse for a broad variety of research fields and is widely used for analytic and diagnostic purposes.

Laboratories and field-based research teams are hampered by the availability of affordable, portable, and low maintenance microplate reader instrumentation. This application note serves to analyze the performance of an ultra-compact microplate reader with established benchtop instrumentation.

To compare both instrument types, a variety of experimental setups have been performed in parallel, using both sandwich and competitive ELISA and the most common ELISA substrates for either type (TMB – tetramethylbenzidine and pNpp-p-Nitrophenylphosphate).