Jürgen Neesen¹, Julia Etzler¹, Morgan Mathieu^2
1HLN-Genetik GmbH, Vienna, Austria
²Enzo Life Sciences, Lausen, Switzerland

Featured Product: CYTAG® CGH Labeling kit

INTRODUCTION

Recent advances in genomics have dramatically increased our capacity to analyze abnormal cells, revealing a multitude of changes in genomic DNA, such as mutations and copy number alterations. Array comparative genomic hybridization (aCGH) has become a valuable, genome-wide screening tool for the detection of chromosomal aberrations in the form of copy number variations (CNVs), notably in the field of cytogenetics.

The major advantage of aCGH is that it offers improved resolution in a rapid and high-throughput fashion compared to traditional techniques. Cytogenetics laboratories rely on this method to identify genetic disorders such as aneuploidy, unbalanced aberrations, and amplification on a minimal amount of DNA in a variety of samples.

HLN-Genetik GmbH conducts cytogenetic analyses on prenatal and postnatal samples. The laboratory has at its disposal a range of chromosomal and molecular techniques to deliver cytogenetic diagnostics. aCGH is amongst the services offered by this laboratory, and it is a technique performed on an InnoScan® 710AL Microarray Scanner from Innopsys.

The main objective of this study was to compare Enzo’s CYTAG® CGH Labeling Kit with Illumina’s Fluorescent Labeling System [dCTP] and look at the compatibility of the staining with Illumina’s 24sure Microarrays. Both labeling kits yielded similar quality control (QC) metrics regardless of the conditions used for labeling and hybridization, demonstrating the compatibility of Enzo’s CYTAG® CGH Labeling Kit with Illumina’s 24sure Microarrays.