Replaces Prod. #: BPD-HYB-314-01
Product Specification
| Alternative Name: | alpha Gliadin |
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| Clone: | 14D5 |
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| Host: | Mouse |
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| Isotype: | IgG2aλ |
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| Immunogen: | Synthetic peptide corresponding to aa Lys57-Glu65-[α-gliadin (58-73)] (KLQPFPQPELPYPQPQ) of Gliadin-related peptide. |
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| Species reactivity: | Plant
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| Applications: | ELISA, WB
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| Application Notes: | Detects a band of ~6 to 10kDa by Western blot. |
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| Purity Detail: | Protein A/G-affinity purified. |
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| Formulation: | Liquid. In 0.01M phosphate buffer, pH 7.4, containing 0.5M sodium chloride and 15mM sodium azide. |
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| Shipping: | Blue Ice Not Frozen |
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| Short Term Storage: | +4°C |
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| Scientific Background: | Celiac disease is associated with a CD4+ T-cell response to epitopes of gliadin presented by HLA-DQ2 or -DQ8 class II MHC molecules. These epitopes are present in a 33-mer peptide of wheat alpha-gliadin, residues 56-88, which is resistant to digestion and forms a substrate for tissue transglutaminase (TG2), generating the glutamic acid residues essential for binding to HLA-DQ2. The immunogen corresponds to a deamidated form of a region that includes the T-cell epitopes, including the immunodominant PQPQLPY region and two PXPQP motifs associated with binding to IgA from patients with celiac disease. Complete homology exists between residues 63-73, 70-80 and 77-87 of wheat alpha gliadin. |
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| Regulatory Status: | RUO - Research Use Only |
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