Product Details
| Alternative Name: | Lymphocyte pore forming protein, PFP, Cytolysin, PRF1 |
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| MW: | 62kDa |
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| Source: | Produced in E.coli. Recombinant human perforin 1 (PRF1) (aa 32-316). |
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| Formulation: | Lyophilized. |
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| Purity: | ≥95% |
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| Endotoxin Content: | <1.0 EU/µg (determined by LAL method) |
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| Reconstitution: | Reconstitute in 20mM Tris, 150mM NaCl (pH 8.0) to a concentration of 0.1-1.0 mg/ml. Do not vortex. |
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| Shipping: | Blue Ice |
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| Short Term Storage: | +4°C |
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| Long Term Storage: | -80°C |
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| Use/Stability: | Stable at 2-8°C for one month. Stable at -80°C for up to 12 months. |
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| Handling: | Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -80°C. |
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| Scientific Background: | Perforin 1 (PRF1) is a pore forming cytolytic protein found in the granules of cytotoxic T lymphocytes (CTLs) and NK cells. Upon degranulation, perforin binds to the target cell's plasma membrane, and oligomerizes in a Ca2+ dependent manner to form pores on the target cell. The pore formed allows for the passive diffusion of a family of pro-apoptotic proteases, known as the granzymes, into the target cell. |
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| Regulatory Status: | RUO - Research Use Only |
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Dose Dependent Binding Activity of PRF1 with CRT. A binding ELISA was performed to detect interaction with Calreticulin (CRT), an interactor of PRF1. PRF1 was serially diluted in PBS and incubated in CRT-coated wells. The binding activity of PRF1 and CRT occurs in a dose dependent manner.
SDS-PAGE of Active Recombinant PRF1, Human
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Product Literature References
Quantifying requirements for mitochondrial apoptosis in CAR T killing of cancer cells: A.L. Pourzia, et al.; Cell Death Dis.
14, 267 (2023),
Abstract;
