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Perforin 1 (human), (recombinant) (aa 32-316)

ENZ-PRT312-0010 10 µg 438.00 USD
ENZ-PRT312-0200 200 µg 2,192.00 USD
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Product Details

Alternative Name:Lymphocyte pore forming protein, PFP, Cytolysin, PRF1
Source:Produced in E.coli. Recombinant human perforin 1 (PRF1) (aa 32-316).
Endotoxin Content:<1.0 EU/µg (determined by LAL method)
Reconstitution:Reconstitute in 20mM Tris, 150mM NaCl (pH 8.0) to a concentration of 0.1-1.0 mg/ml. Do not vortex.
Shipping:Blue Ice
Short Term Storage:+4°C
Long Term Storage:-80°C
Use/Stability:Stable at 2-8°C for one month. Stable at -80°C for up to 12 months.
Handling:Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -80°C.
Scientific Background:Perforin 1 (PRF1) is a pore forming cytolytic protein found in the granules of cytotoxic T lymphocytes (CTLs) and NK cells. Upon degranulation, perforin binds to the target cell's plasma membrane, and oligomerizes in a Ca2+ dependent manner to form pores on the target cell. The pore formed allows for the passive diffusion of a family of pro-apoptotic proteases, known as the granzymes, into the target cell.
Regulatory Status:RUO - Research Use Only
Perforin 1 (human), (recombinant) (aa 32-316) Binding Activity
Dose Dependent Binding Activity of PRF1 with CRT. A binding ELISA was performed to detect interaction with Calreticulin (CRT), an interactor of PRF1. PRF1 was serially diluted in PBS and incubated in CRT-coated wells. The binding activity of PRF1 and CRT occurs in a dose dependent manner.
Perforin 1 (human), (recombinant) (aa 32-316) SDS-PAGE
SDS-PAGE of Active Recombinant PRF1, Human
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Perforin 1 (human), (recombinant) (aa 32-316) Binding Activity Perforin 1 (human), (recombinant) (aa 32-316) SDS-PAGE

Product Literature References

Quantifying requirements for mitochondrial apoptosis in CAR T killing of cancer cells: A.L. Pourzia, et al.; Cell Death Dis. 14, 267 (2023), Abstract;

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