| MW: | ~56 kDa |
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| Source: | Produced in E. coli. Human 14-3-3ε is fused at the C-terminus to a GST-His-tag. |
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| UniProt ID: | P62258 |
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| Formulation: | Liquid. In 20 mM Tris-HCl, pH 8.0, containing 100 mM NaCl. |
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| Purity: | ≥90% (SDS-PAGE) |
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| Purity Detail: | Purified by multi-step chromatography. |
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| Application Notes: | Drug screening, protein-protein interaction studies, gel overlays. |
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| Shipping: | Dry Ice |
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| Long Term Storage: | -80°C |
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| Handling: | Avoid freeze/thaw cycles. |
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| Scientific Background: | 14-3-3 proteins are dimeric phosphoserine/phosphothreonine binding proteins, which have been shown to be involved in the regulation of many crucial processes such as signal transduction, cell-cycle control, apoptosis, protein localization, phosphorylation state and stability, transcription, metabolism, and malignant transformation. 14-3-3ε is implicated in an early left-right patterning in amphibian embryos. In addition, children with Miller-Dieker syndrome, a severe brain malformation associated with mental retardation, have heterozygous deletions of both the 14-3-3ε and LIS1 genes. It has also been shown to be the only 14-3-3 isoform associated with prion protein amyloid deposits of Gerstmann-Straussler-Scheinker disease. |
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| Regulatory Status: | RUO - Research Use Only |
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