~23 kDa (2 other immunoreactive bands are present at ~16 kDa and ~6 kDa)
Source:
Produced in HEK cells. The human growth hormone (aa 27-217) is fused at the N-terminus to a FLAG®-tag followed by an 8 AA linker.
UniProt ID:
P01241
Formulation:
Lyophilized in PBS.
Purity:
≥90% (SDS-PAGE)
Endotoxin Content:
<0.1 EU/µg protein (LAL test; Bio Whittaker)
Biological Activity:
Induces proliferation of Rat Nb2-11 cells (ED50 range is 0.02ng/ml-0.5ng/ml).
Reconstitution:
Reconstitute in 100µl deionized water for a final concentration of 0.1mg/ml.
Shipping:
Blue Ice Not Frozen
Long Term Storage:
-20°C
Handling:
Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -80°C.
Scientific Background:
Human growth hormone is a growth factor that has been shown to play and important role in the growth control of cells. It is a member of a group of hormones produced by the pituitary gland that also include prolactin. Human Growth hormone has been shown to stimulate proliferation of Nb2-11cells in a dose dependent manner. Its ability to promote growth is mediated by the somatomedin family of peptide hormones. It has been shown to enhance glucose transport and facilitates glycogen storage, as well as the ability to stimulate IGF-1 secretion in liver and other tissue.
Technical Info/Product Notes:
FLAG is a registered trademark of Sigma-Aldrich Co.
Regulatory Status:
RUO - Research Use Only
Western Blot analysis of Human Growth Hormone probed with rabbit anti-FLAG antibody: Lane 1: MWM, Lane 2: 100 ng Human Growth Hormone (ENZ-PRT189) probed with anti-FLAG.
Coomassie stained SDS-PAGE. Lane 1, Molecular weight marker. Lane 2, 1.0µg Human Growth Hormone (ENZ-PRT189).
Nb2-11 cell proliferation assay after treatment with Human Growth Hormone: Nb2-11 cells were placed in stationary media at 50,000cells/well in 96 well plates. They were treated with a tittered concentration of Human Growth hormone. The cells were grown at 37°C / 5% CO2 for 48 hours. Proliferation was measured using an MTT cell viability assay. The ED50 range is around 0.02 – 0.5 ng/ml.
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General Literature References
Sequence-Function Relationships Within the Expanding Family of Prolactin, Growth Hormone, Placental Lactogen, and Related Proteins in Mammals: V. Goffin, et al.; Endocr. Rev. 17, 385 (1996), Abstract;
Human growth hormone and prolactin secreting pituitary adenomas analyzed by in situ hybridization: R.V. Lloyd, et al.; Am. J. Pathol. 134, 605 (1989), Abstract;
Growth hormone and insulin-like growth factors I and II produce distinct alterations in glucose metabolism in 3T3-F442A adipocytes: J. Schwartz, et al.; PNAS 82, 8724 (1985), Abstract;
Prolactin-stimulated growth of cell cultures established from malignant Nb rat lymphomas: P.W. Gout, et al.; Cancer Res. 40, 2433 (1980), Abstract;
