As part of the need to increase the sensitivity of your sandwich ELISA, this streptavidin AP conjugate can be used with your sandwich ELISA that utilizes a biotinylated detector antibody and the AMP'D® ELISA Signal Amplification kit (Prod. no. ENZ-KIT-100) which requires the presence of the AP as the conjugate.
Product Specification
| Source: | Native. Purified from Streptomyces avidinii (SA) and calf intestine (AP). |
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| Formulation: | Liquid (50 μg/ml). In 0.025M TRIS-HCl, pH 8.0, containing 0.075M sodium chloride, 0.05mM ZnSO4, 2.45mM MgCl2, 0.045% sodium azide, 0.05% BSA and 50% Stabilzyme-AP. |
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| Purity Detail: | Alkaline phosphatase purified from calf intestine. |
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| Applications: | ELISA
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| Application Notes: | For certified AMP'D® ELISA kit development. The recommended concentration in an ELISA would be 500-100ng/ml, which is a 1:100 to 1:500 dilution of the sample provided.
Optimal conditions must be determined individually for each application. |
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| Shipping: | Shipped on Blue Ice |
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| Long Term Storage: | -20°C |
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| Regulatory Status: | RUO - Research Use Only |
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General Literature References
The critical roles of endoplasmic reticulum chaperones and unfolded protein response in tumorigenesis and anticancer therapies: B. Luo & A.S. Lee; Oncogene
32, 805 (2013), (Review),
Abstract;
Full Text
The critical role of GRP78 in physiologic and pathologic stress: K.T. Pfaffenbach & A.S. Lee; Curr. Opin. Cell Biol.
23, 150 (2011), (Review),
Abstract;
Full Text
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