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NEDD8 activating enzyme E1 (human), (recombinant) (His-tag)

ENZ-PRT112-0050 50 µg 248.00 USD
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Product Details

Alternative Name:NEDD8-activating enzyme E1 catalytic subunit, NEDD8-activating enzyme E1C, Ubiquitin-activating enzyme E1C, Ubiquitin-like modifier-activating enzyme 3, Ubiquitin-activating enzyme 3
MW:62kDa + 53kDa (heterodimer: 112.15kDa)
Source:Produced in E. coli.
UniProt ID:Q8TBC4
Formulation:Liquid. In 25mM HEPES, pH 7.5, containing 150mM NaCl, 1mM DTT.
Purity:≥95% (SDS-PAGE)
Purity Detail:Copurified in His-tagged form with Uba3; copurified with APP-BP1.
Application Notes:For use in NEDD8 conjugation reactions and NEDDylation pathway studies.
Shipping:Shipped on Dry Ice
Long Term Storage:-80°C
Scientific Background:Conjugation of mature NEDD8 to specific lysine residues on target proteins via isopeptide bonds allows NEDD8 to play a critical regulatory role in cell proliferation and development, a process closely associated to the ubiquitin-proteasome pathway. An increasing number of substrates for NEDDylation are being identified including the tumor suppressors p53 and VHL and members of the well-characterized cullin ubiquitin E3 ligase family, underlining the vital role NEDD8 modification plays in cellular processes.
Regulatory Status:RUO - Research Use Only
NEDD8 activating enzyme E1 (human), (recombinant) (His-tag) SDS-PAGE
SDS-PAGE Analysis. Lane 1: MW Marker; Lane 2: 1 ug NEDD8 E1.
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NEDD8 activating enzyme E1 (human), (recombinant) (His-tag) SDS-PAGE

Product Literature References

Structural basis for HIV-1 antagonism of host APOBEC3G via Cullin E3 ligase: F. Ito, et al.; Sci. Adv. 9, eade3168 (2023), Abstract;
Reconstitution of the destruction complex defines roles of AXIN polymers and APC in β-catenin capture, phosphorylation, and ubiquitylation: M. Ranes, et al.; Mol. Cell 81, 3246 (2021), Abstract;

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