Copurified in His-tagged form with Uba3; copurified with APP-BP1.
Application Notes:
For use in NEDD8 conjugation reactions and NEDDylation pathway studies.
Shipping:
Shipped on Dry Ice
Long Term Storage:
-80°C
Scientific Background:
Conjugation of mature NEDD8 to specific lysine residues on target proteins via isopeptide bonds allows NEDD8 to play a critical regulatory role in cell proliferation and development, a process closely associated to the ubiquitin-proteasome pathway. An increasing number of substrates for NEDDylation are being identified including the tumor suppressors p53 and VHL and members of the well-characterized cullin ubiquitin E3 ligase family, underlining the vital role NEDD8 modification plays in cellular processes.
Regulatory Status:
RUO - Research Use Only
SDS-PAGE Analysis. Lane 1: MW Marker; Lane 2: 1 ug NEDD8 E1.
