Amplification of challenging GC and AT-rich templates to over 17.5kb
Hot start technology for maximized sensitivity and specificity
AMPIGENE® Hot Start High Fidelity Polymerase was engineered to improve DNA binding resulting in amplification of difficult targets, greater yields, and shorter extension times.
AMPIGENE® Hot Start High Fidelity Polymerase Provides Enhanced Performance with Difficult Templates AMPIGENE® Hot Start High Fidelity Polymerase exhibited superior performance at different annealing temperatures (Ta1 = 68.5°C, Ta2 = 66.0°C, Ta3 = 63.0°C, Ta4 = 60.5°C) compared to the competitors’ product. AMPIGENE® Hot Start High Fidelity Polymerase generated cleaner bands and no primer dimers. Human gDNA, 30 ng (8333 copies) and 13500 bp long was used as template.
AMPIGENE® Hot Start High Fidelity Polymerase Provides Enhanced Performance with GC-Rich Templates AMPIGENE® Hot Start High Fidelity Polymerase exhibited superior performance with GC-rich template in comparison to the competitors’ product. The template used was 500 pb long with 69% GC content.
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Product Details
Applications:
PCR
Shipping:
Dry Ice
Long Term Storage:
-20°C
Contents:
AMPIGENE® Hot Start High Fidelity Polymerase (2U/μl), 5x AMPIGENE® High Fidelity Rxn buffer, 10x AMPIGENE® Enhancer
