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AMPIGENE® Hot Start High Fidelity Polymerase

Optimized Hot Start High Fidelity Polymerase for greater sequence accuracy
 
ENZ-NUC133-0100 100 U 144.00 USD
 
ENZ-NUC133-0500 500 U 638.00 USD
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  • 100x greater fidelity than Taq DNA polymerase
  • Increased processivity (10-30s/kb)
  • Amplification of challenging GC and AT-rich templates to over 17.5kb
  • Hot start technology for maximized sensitivity and specificity
AMPIGENE® Hot Start High Fidelity Polymerase was engineered to improve DNA binding resulting in amplification of difficult targets, greater yields, and shorter extension times.
AMPIGENE Hot Start High Fidelity Polymerase performance difficult and long templates
AMPIGENE® Hot Start High Fidelity Polymerase Provides Enhanced Performance with Difficult Templates
AMPIGENE® Hot Start High Fidelity Polymerase exhibited superior performance at different annealing temperatures (Ta1 = 68.5°C, Ta2 = 66.0°C, Ta3 = 63.0°C, Ta4 = 60.5°C) compared to the competitors’ product. AMPIGENE® Hot Start High Fidelity Polymerase generated cleaner bands and no primer dimers. Human gDNA, 30 ng (8333 copies) and 13500 bp long was used as template.
AMPIGENE Hot Start High Fidelity Polymerase performance GC-rich templates
AMPIGENE® Hot Start High Fidelity Polymerase Provides Enhanced Performance with GC-Rich Templates
AMPIGENE® Hot Start High Fidelity Polymerase exhibited superior performance with GC-rich template in comparison to the competitors’ product. The template used was 500 pb long with 69% GC content.
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AMPIGENE Hot Start High Fidelity Polymerase performance difficult and long templates AMPIGENE Hot Start High Fidelity Polymerase performance GC-rich templates

Product Details

Applications:PCR
 
Shipping:Dry Ice
 
Long Term Storage:-20°C
 
Contents:AMPIGENE® Hot Start High Fidelity Polymerase (2U/μl), 5x AMPIGENE® High Fidelity Rxn buffer, 10x AMPIGENE® Enhancer
 
Regulatory Status:RUO - Research Use Only
 

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