Digoxigenin-dUTP replaces dTTP in reactions in which it serves as a substrate for E. coli DNA polymerase (holoenzyme and Klenow fragment), T4 and Taq DNA polymerases, reverse transcriptase (from AMV and M-MuLV) and terminal transferase. Digoxigenin-dUTP can be used to produce digoxigenin-labeled DNA probes in a variety of labeling reactions including nick translation, random prime labeling, cDNA labeling and 3’-end labeling. The resulting digoxigenin-labeled probe can be used in a variety of hybridization applications incuding Southern blots, Northern blots, or dot blots. The probes can also be used for in situ hybridization procedures on fixed cells and tissues. Digoxigenin-labeled probes have been shown to hybridize to homologous nucleic acid at the same rate and to the same extent as non-labeled probes. The hybridized digoxigenin-labeled DNA probes can be detected by their interaction with antibodies coupled to fluorescent dyes or color-producing enzymes.
This labeled dUTP can be used with the Nick Translation DNA Labeling System 2.0 (Prod. No. ENZ-GEN111).
Product Details
Alternative Name: | Digoxigenin-11-dUTP |
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Formula: | C43H65N4O21P3 |
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MW: | 1066.92 (free acid) |
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Quantity: | Sufficient for approximately 25 reactions, following the recommended protocol of Prod. No. ENZ-GEN111. |
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Concentration: | 1mM |
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Formulation: | Liquid. Solution in water. |
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Extinction Coefficient: | 22,600 M-1cm-1 (220nm, pH 7) |
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Purity: | ≥93% (HPLC) |
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Appearance: | Clear, colorless liquid. |
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Applications: | ISH (in situ hybridization), Southern Blot
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Shipping: | Dry Ice |
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Long Term Storage: | -20°C |
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Handling: | Avoid freeze/thaw cycles. |
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Regulatory Status: | RUO - Research Use Only |
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Product Literature References
Novel selectively amplified DNA sequences in the germline genome of the Japanese hagfish, Eptatretus burgeri: K. Nagao, et al.; Sci. Rep.
12, 21373 (2022),
Abstract;
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