For dye-based qPCR applications: Green dye does not interfere with PCR
Optimized buffer system for efficient amplification
Enhancers and stabilizers improve reaction sensitivity and specificity
AMPIGENE® qPCR 1-Step Green Kits use the latest developments in reverse transcriptase (RTase) technology and buffer chemistry for efficient cDNA synthesis and qPCR in a single tube. The modified MMLV RTase is blended with RNase inhibitor to prevent degradation of RNA by contaminating RNase. The RTase is thermostable and extremely active. AMPIGENE® qPCR 1-Step Green Kits use a proprietary intercalating dye that does not inhibit PCR. An optimized buffer system allows efficient amplification and proprietary enhancers prevent the formation of primers/dimers to increase sensitivity and specificity. ROX is a reference dye which is used to normalize fluorescent signal in qPCR. AMPIGENE® qPCR 1-Step Green Kits come with either Lo-ROX or Hi-ROX concentration. For Hi-ROX, see Prod. No. ENZ-NUC109. Choose the appropriate ROX concentration for the instrument (see instrument compatibility for reference dye chart below.)
Instrument compatibility for ROX dye.
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Product Details
Applications:
PCR
Application Notes:
For cDNA synthesis and dye-based qPCR in a single tube
Shipping:
Shipped on Blue Ice
Long Term Storage:
-20°C
Contents:
2x AMPIGENE® qPCR 1-Step Green Mix Lo-ROX (includes dNTPs and polymerases)
20x RTase (with RNase inhibitor)
