Optimized buffer system for efficient amplification
Enhancers and stabilizers improve reaction sensitivity and specificity
AMPIGENE® qPCR Green Mixes use the latest developments in polymerase technology and buffer chemistry to deliver high performing dye-based real-time PCR with minimal optimization. AMPIGENE® qPCR Green Mixes use a proprietary intercalating dye that does not inhibit PCR. An optimized buffer system allows efficient amplification and proprietary enhancers prevent the formation of primers/dimers to increase sensitivity and specificity. ROX is a reference dye which is used to normalize fluorescent signal in qPCR. AMPIGENE® qPCR Green Mixes come with either Lo-ROX or Hi-ROX concentration. For Hi-ROX, see Prod. No. ENZ-NUC104. Choose the appropriate ROX concentration for the instrument (see instrument compatibility for reference dye chart below.)
Instrument compatibility for Rox dye.
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Product Details
Applications:
PCR
Application Notes:
For dye-based qPCR
Shipping:
Blue Ice
Long Term Storage:
-20°C
Contents:
2x AMPIGENE® qPCR Green Mix Lo-ROX (includes dNTPs and polymerases)
Regulatory Status:
RUO - Research Use Only
Product Literature References
Anticancer activity of IRAK-4 inhibitors against canine lymphoid malignancies: J.H. Park, et al.; Vet. Comp. Oncol. 20, 632 (2022), Abstract;
Drenched Silicon Suppresses Disease and Insect Pests in Coffee Plant Grown in Controlled Environment by Improving Physiology and Upregulating Defense Genes: J. Yang, et al.; Int. J. Mol. Sci. 23, 3543 (2022), Abstract;
Effect of Cirsium japonicum Flower Extract on Skin Aging Induced by Glycation: S. Yoon, et al.; Molecules 27, 2093 (2022), Abstract;
Influenza A pathway analysis of highly pathogenic avian influenza virus (H5N1) infection in genetically disparate Ri chicken lines: J. Lee, et al.; Vet. Immunol. Immunopathol. 246, 110404 (2022), Abstract;
Lighting from Top and Side Enhances Photosynthesis and Plant Performance by Improving Light Usage Efficiency: J. Yang, et al.; Int. J. Mol. Sci. 23, 2448 (2022), Abstract;
Immunomodulatory effects of poly(I:C)-stimulated exosomes derived from chicken macrophages: Y. Hong, et al.; Poult. Sci. 100, 101247 (2021), Abstract;
Interaction between hypoviral-regulated fungal virulence factor laccase3 and small heat shock protein Hsp24 from the chestnut blight fungus Cryphonectria parasitica: J. Chun, et al.; J. Microbiol. 60, 57 (2021), Abstract;
Measuring the absolute abundance of the microbiome by adding yeast containing 16S rRNA gene from a hyperthermophile: J.Y. Kim, et al.; Microbiologyopen 10, e1220 (2021), Abstract;
Senotherapeutic-like effect of Silybum marianum flower extract revealed on human skin cells: J. Woo, et al.; PLoS One 16, e0260545 (2021), Abstract;
Transcriptomic analysis of the liver in aged laying hens with different intensity of brown eggshell color: G.P. Han, et al.; Anim. Biosci. 34, 811 (2021), Abstract; Full Text
Cryptotanshinone chemosensitivity potentiation by TW-37 in human oral cancer cell lines by targeting STAT3-Mcl-1 signaling: I. Yang, et al.; Cancer Cell Int. 20, 405 (2020), Abstract; Full Text
High-intensity Exercise Improves Cognitive Function and Hippocampal Brain-Derived Neurotrophic Factor Expression in Obese Mice Maintained on High-Fat Diet: T. Kim, et al.; J. Exerc. Rehabil. 16, 124 (2020), Abstract; Full Text
Peptoid-Substituted Hybrid Antimicrobial Peptide Derived from Papiliocin and Magainin 2 with Enhanced Bacterial Selectivity and Anti-inflammatory Activity: A. Shin, et al.; Biochemistry 54, 3924 (2020), Abstract; Full Text
Triple herbal extract DA-9805 exerts a neuroprotective effect via amelioration of mitochondrial damage in experimental models of Parkinson's disease: J.S. Jeong, et al.; Sci. Rep. 8, 15953 (2018), Abstract; Full Text
Combined therapy with oncolytic adenoviruses encoding TRAIL and IL-12 genes markedly suppressed human hepatocellular carcinoma both in vitro and in an orthotopic transplanted mouse model: A.G. El-Shemi, et al.; J. Exp. Clin. Cancer Res. 35, 74 (2016), Abstract;
