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Neomycin ELISA kit

Simple, fast, and sensitive method for detection of Neomycin in cell culture and tissue
ENZ-KIT217-0001 96 wells 395.00 USD
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  • Highly specific ELISA which does not detect streptomycin, kanamicin or gentamicin, providing confidence in assay results
  • Sensitive measurement, detecting as little as 0.5 ppb (ng/mL) neomycin
  • Cost effective and fast, with results in less than 1h 
  • Easy-to-use liquid color-coded reagents and pre-coated plates minimize error
The Neomycin ELISA kit is a colorimetric competitive enzyme immunoassay kit with results in less than 1 hour. Absorbance is read at 450 or 630 nm. Very low reactivity with other antibiotics provides confidence in assay results. Sensitive quantification of 0.5 ng/mL neomycin. Reagents and protocol for sample extraction is included in the kit.
Neomycin ELISA Kit Standard curve
Standard curve of Neomycin ELISA Kit
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Neomycin ELISA Kit Standard curve

Product Details

Sensitivity:0.5 ppb (ng/ml) 
Assay Time:45 min
Applications:ELISA, Colorimetric detection
Application Notes:For the quantitative determination of neomycin in cell culture supernatants, muscle, milk, egg.
Wavelength:450nm / 630nm
Species reactivity:Species independent
Crossreactivity:Streptomycin, Kanamycin, Gentamicin < 0.1%
Use/Stability:Store all components at 2-8°C
Handling:Do not freeze
Shipping:Blue Ice
Long Term Storage:+4°C
Contents:Microtiter plate, Conjugate, Antibody Working Solution, Substrate Reagents, Concentrated Sample Solution,  Wash Buffer Concentrate, Standard, Stop solution. Additional: Concentrated Tissue Extraction Solution
Scientific Background:Neomycin is an antibiotic that displays bactericidal activity against gram-negative bacteria and is partially effective against gram positive bacteria. Neomycin belongs to the aminoglycoside class of antibiotics that contain two or more amino sugars connected by glycosidic bonds. All aminoglycosides are potentially toxic compounds. Neomycin is included, in small quantities, as a preservative in some vaccines.
Regulatory Status:RUO - Research Use Only
Compatibility:This product is compatible with the Absorbance 96 Plate Reader.

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Competitive immunoassay for the detection of streptomycin in cell culture supernatants and tissue samples
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