Biotin-free nanopolymer-based system for superior multiplex antigen detection
Complete kit includes HIGHDEF® Chromogens for sensitive, crisp 2-color development
Preserves precious tissue while reducing reagent and labor costs
Expandable with additional chromogens (sold separately)
The MULTIVIEW® PLUS (mouse-HRP/rabbit-AP) IHC Kit is a non-biotin one-step detection system suitable for detecting and localizing antigens in formalin-fixed paraffin-embedded tissues.
Mouse anti-CD44 clone SFF-304 and mouse anti-Ki-67 clone MIB-1 with Rabbit anti-Mouse Linker (Cat. No. ENZ-ACC116) was used with MULTIVIEW® PLUS (mouse-HRP/rabbit-AP) Kit (Prod. No. ENZ-KIT181) to perform multiplex immunohistochemistry staining with hematoxylin used as a counterstain. Tissue used is human buttock.
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Product Details
Assay Time:
~2 hours
Applications:
IHC
Shipping:
Blue Ice
Long Term Storage:
+4°C
Contents:
Antigen Retrieval Reagent, pH 9 (10X), ISH/IHC Peroxidase Block, Antibody Blocker/Diluent, MULTIVIEW® PLUS HRP (Anti-Mouse) Reagent, MULTIVIEW® PLUS AP (Anti-Rabbit) Reagent, HIGHDEF® DAB Chromogen, HIGHDEF® DAB Substrate, HIGHDEF® Green AP Chromogen, HIGHDEF® Green AP Substrate, Hematoxylin, Empty Mixing Bottle
The MULTIVIEW® PLUS (mouse-HRP/rabbit-AP) IHC Kit (Brown/Green) provides reagents and materials for the ultrasensitive multiplex detection of mouse and rabbit antibodies used in immunohistochemistry (IHC) staining. The kit is a non-biotin detection system suitable for identifying antigens in formalin-fixed paraffin-embedded tissues and frozen sections. This kit may also be used with blood smears, cytosmears, and cell preparations. MULTIVIEW® PLUS (mouse-HRP/rabbit-AP) IHC Kit (Brown/Green) contains both MULTIVIEW® PLUS HRP (Anti-Mouse) Reagent and MULTIVIEW® PLUS AP (Anti-Rabbit) Reagent, which have been developed by directly labeling anti-mouse immunoglobulins with enzymes using a proprietary tandem hyper-labelling technology in a ready-to-use format. This ensures consistent and reproducible immunodetection of mouse and rabbit antibodies against nuclear, cytoplasmic, and membrane antigens in different types of tissues and cells. The MULTIVIEW® PLUS HRP (Anti-Mouse) Reagent and MULTIVIEW® PLUS AP (Anti-Rabbit) Reagent enable faster staining procedures compared to traditional two-step methods using biotin and avidin/streptavidin conjugates, with significantly lower background.
Protocol:
MULTIVIEW® 4-color staining protocol available through our Technical Support webpages.
Regulatory Status:
RUO - Research Use Only
Product Literature References
Anacardic acid induces IL-33 and promotes remyelination in CNS: A. Ljunggren-Rose, et al.; PNAS 117, 21527 (2020), Abstract; Full Text
MiR-210 is overexpressed in tumor-infiltrating plasma cells in triple-negative breast cancer: I. Bar, et al.; J. Histochem. Cytochem. 68, 25 (2020), Abstract; Full Text
Inflammatory chemokine profiles and their correlations with effector CD4 T cell and regulatory cell subpopulations in cutaneous lupus erythematosus: S. Mendez-Flores, et al.; Cytokine 119, 95 (2019), Application(s): IHC on skin biopsies , Abstract;
Neuron/glial antigen 2-type VI collagen interactions during murine temporomandibular joint osteoarthritis: M. Yotsuya, et al.; Sci. Rep. 9, 56 (2019), Abstract; Full Text
Two-photon fluorescence and second harmonic generation characterization of extracellular matrix remodeling in post-injury murine temporomandibular joint osteoarthritis: D.A. Reed, et al.; PLoS One 14, e0214072 (2019), Abstract; Full Text
Caspase recruitment domain (CARD) family (CARD9, CARD10, CARD11, CARD14 and CARD15) are increased during active inflammation in patients with inflammatory bowel disease: J.K. Yamamoto-Furusho, et al.; J. Inflamm. (Lond.) 15, 13 (2018), Abstract; Full Text
Differentiation patterns of uterine carcinomas and precursor lesions induced by neonatal estrogen exposure in mice: A.A. Suen, et al.; Toxicol. Path. 46, 574 (2018), Abstract; Full Text
RORγt-expressing Tregs drive the growth of colitis-associated colorectal cancer by controlling IL6 in dendritic cells: A. Rizzo, et al.; Cancer Immunol. Res. 6, 1082 (2018), Abstract;
