Online Purchasing Account You are logged on as Guest. LoginRegister a New AccountShopping cart (Empty)
United States 

MULTIVIEW® PLUS (mouse-HRP/rabbit-AP) IHC Kit (Brown/Green)

Superior multiplex IHC kit for  efficient detection of multiple antigens in a single tissue sample.
 
ENZ-KIT181-0150 150 tests Inquire for pricing
Do you need bulk/larger quantities?
 
Available from stock, Ready for shipment.
  • Biotin-free nanopolymer-based system for superior multiplex antigen detection
  • Complete kit includes HIGHDEF® Chromogens for sensitive, crisp 2-color development
  • Preserves precious tissue while reducing reagent and labor costs
  • Expandable with additional chromogens (sold separately)
The MULTIVIEW® PLUS (mouse-HRP/rabbit-AP) IHC Kit is a non-biotin one-step detection system suitable for detecting and localizing antigens in formalin-fixed paraffin-embedded tissues.
MULTIVIEW® PLUS (mouse-HRP/rabbit-AP) IHC Kit (Brown/Green) kit
MULTIVIEW® PLUS (mouse-HRP/rabbit-AP) IHC Kit (Brown/Green) Immunohistochemistry
Mouse anti-CD44 clone SFF-304 and mouse anti-Ki-67 clone MIB-1 with Rabbit anti-Mouse Linker (Cat. No. ENZ-ACC116) was used with MULTIVIEW® PLUS (mouse-HRP/rabbit-AP) Kit (Prod. No. ENZ-KIT181) to perform multiplex immunohistochemistry staining with hematoxylin used as a counterstain. Tissue used is human buttock.
Please mouse over
MULTIVIEW® PLUS (mouse-HRP/rabbit-AP) IHC Kit (Brown/Green) kit MULTIVIEW® PLUS (mouse-HRP/rabbit-AP) IHC Kit (Brown/Green) Immunohistochemistry

Product Details

Assay Time:~2 hours
 
Applications:IHC
 
Shipping:Blue Ice Not Frozen
 
Long Term Storage:+4°C
 
Contents:Antigen Retrieval Reagent, pH 9 (10X), ISH/IHC Peroxidase Block, Antibody Blocker/Diluent, MULTIVIEW® PLUS HRP (Anti-Mouse) Reagent, MULTIVIEW® PLUS AP (Anti-Rabbit) Reagent, HIGHDEF® DAB Chromogen, HIGHDEF® DAB Substrate, HIGHDEF® Green AP Chromogen, HIGHDEF® Green AP Substrate, Hematoxylin, Empty Mixing Bottle
 
Scientific Background:Application Note
Three-color IHC Staining On Free-floating Sections From Human Brain
Tumor Phenotyping and Immune Cell Regulation using Multiplex IHC
 
Technical Info/Product Notes:The MULTIVIEW® PLUS (mouse-HRP/rabbit-AP) IHC Kit (Brown/Green) provides reagents and materials for the ultrasensitive multiplex detection of mouse and rabbit antibodies used in immunohistochemistry (IHC) staining. The kit is a non-biotin detection system suitable for identifying antigens in formalin-fixed paraffin-embedded tissues and frozen sections. This kit may also be used with blood smears, cytosmears, and cell preparations. MULTIVIEW® PLUS (mouse-HRP/rabbit-AP) IHC Kit (Brown/Green) contains both MULTIVIEW® PLUS HRP (Anti-Mouse) Reagent and MULTIVIEW® PLUS AP (Anti-Rabbit) Reagent, which have been developed by directly labeling anti-mouse immunoglobulins with enzymes using a proprietary tandem hyper-labelling technology in a ready-to-use format. This ensures consistent and reproducible immunodetection of mouse and rabbit antibodies against nuclear, cytoplasmic, and membrane antigens in different types of tissues and cells. The MULTIVIEW® PLUS HRP (Anti-Mouse) Reagent and MULTIVIEW® PLUS AP (Anti-Rabbit) Reagent enable faster staining procedures compared to traditional two-step methods using biotin and avidin/streptavidin conjugates, with significantly lower background.
 
Protocol:MULTIVIEW® 4-color staining protocol available through our Technical Support webpages.
 
Regulatory Status:RUO - Research Use Only
 

Product Literature References

Anacardic acid induces IL-33 and promotes remyelination in CNS: A. Ljunggren-Rose, et al.; PNAS 117, 21527 (2020), Abstract; Full Text
MiR-210 is overexpressed in tumor-infiltrating plasma cells in triple-negative breast cancer: I. Bar, et al.; J. Histochem. Cytochem. 68, 25 (2020), Abstract; Full Text
Inflammatory chemokine profiles and their correlations with effector CD4 T cell and regulatory cell subpopulations in cutaneous lupus erythematosus: S. Mendez-Flores, et al.; Cytokine 119, 95 (2019), Application(s): IHC on skin biopsies , Abstract;
Neuron/glial antigen 2-type VI collagen interactions during murine temporomandibular joint osteoarthritis: M. Yotsuya, et al.; Sci. Rep. 9, 56 (2019), Abstract; Full Text
Two-photon fluorescence and second harmonic generation characterization of extracellular matrix remodeling in post-injury murine temporomandibular joint osteoarthritis: D.A. Reed, et al.; PLoS One 14, e0214072 (2019), Abstract; Full Text
Caspase recruitment domain (CARD) family (CARD9, CARD10, CARD11, CARD14 and CARD15) are increased during active inflammation in patients with inflammatory bowel disease: J.K. Yamamoto-Furusho, et al.; J. Inflamm. (Lond.) 15, 13 (2018), Abstract; Full Text
Differentiation patterns of uterine carcinomas and precursor lesions induced by neonatal estrogen exposure in mice: A.A. Suen, et al.; Toxicol. Path. 46, 574 (2018), Abstract; Full Text
RORγt-expressing Tregs drive the growth of colitis-associated colorectal cancer by controlling IL6 in dendritic cells: A. Rizzo, et al.; Cancer Immunol. Res. 6, 1082 (2018), Abstract;

Related Products

POLYVIEW® PLUS HRP (anti-rabbit) reagent 

High sensitivity, low background nanopolymer detection reagent for use with HIGHDEF® chromogens in ISH and IHC applications
IHC, ISH (in situ hybridization) | Print as PDF
 
ENZ-ACC103-0150 150 tests Inquire for pricing
Do you need bulk/larger quantities?
 

POLYVIEW® PLUS HRP (anti-mouse) reagent 

High sensitivity, low background nanopolymer detection reagent for use with HIGHDEF® chromogens in ISH and IHC applications
IHC, ISH (in situ hybridization) | Print as PDF
 
ENZ-ACC104-0150 150 tests Inquire for pricing
Do you need bulk/larger quantities?
 

POLYVIEW® PLUS AP (anti-rabbit) reagent 

High sensitivity, low background nanopolymer detection reagent for use with HIGHDEF® chromogens in ISH and IHC applications
IHC, ISH (in situ hybridization) | Print as PDF
 
ENZ-ACC110-0150 150 tests Inquire for pricing
Do you need bulk/larger quantities?
 

SAVIEW® PLUS AP Reagent 

High sensitivity, low background, streptavidin-based nanopolymer detection reagent for use with HIGHDEF® chromogens in ISH and IHC applications
IHC, ISH (in situ hybridization) | Print as PDF
 
ENZ-ACC111-0150 150 tests Inquire for pricing
Do you need bulk/larger quantities?
 

HIGHDEF® DAB Chromogen/Substrate Set 

Brown chromogen for use with HRP immunohistochemistry staining systems
IHC, ISH (in situ hybridization) | Print as PDF
 
ENZ-ACC105-0200 200 ml Inquire for pricing
Do you need bulk/larger quantities?
 

HIGHDEF® Hematoxylin 

Ready-to-use histologic stain with enhancers
IHC, ISH (in situ hybridization) | Print as PDF
 
ENZ-ACC106-0100 100 ml Inquire for pricing
Do you need bulk/larger quantities?
 

IHC/ISH Peroxidase Block 

IHC, ISH (in situ hybridization) | Print as PDF
 
ENZ-ACC107-0100 100 ml Inquire for pricing
Do you need bulk/larger quantities?
 

Antibody Blocker/Diluent 

Ready-to-use antibody blocking buffer and diluent, for use with POLYVIEW® PLUS detection reagents
IHC, ISH (in situ hybridization) | Print as PDF
 
ENZ-ACC108-0100 100 ml Inquire for pricing
Do you need bulk/larger quantities?
 

Antigen Retrieval Reagent, pH 6 (10X) 

IHC | Print as PDF
 
ENZ-ACC112-0100 100 ml Inquire for pricing
Do you need bulk/larger quantities?
 

Antigen Retrieval Reagent, pH 9 (10X) 

IHC | Print as PDF
 
ENZ-ACC113-0100 100 ml Inquire for pricing
Do you need bulk/larger quantities?
 

HIGHDEF® blue IHC chromogen (AP) 

High definition IHC chromogen producing brilliant blue color for AP-activated development.
IHC, ISH (in situ hybridization) | Print as PDF
 
ADI-950-150-0030 30 ml Inquire for pricing
Do you need bulk/larger quantities?
 

Product Toolbox

PRODUCT RESOURCES

Datasheet
MSDS
Certificate of Analysis
Manual

RELATED PRODUCTS

By catalog section:

PRODUCT SUPPORT

FAQs
Technical Service
Customer Service

Related Literature

Brochures
Live Cell Analysis
Live Cell Analysis
Download as PDF

Brochures
Immunohistochemistry - Complete solutions for IHC analysis
Immunohistochemistry - Complete solutions for IHC analysis
Download as PDF

Brochures
Neuroscience Antibodies for IHC
Neuroscience Antibodies for IHC
Download as PDF

Product Flyers
Ultra-sensitive Multiplex Detection
Ultra-sensitive Multiplex Detection
Download as PDF

All new literature pieces

Recommend this page

 
Keep in touch

©2021 Enzo Life Sciences, Inc.,