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Removes gDNA from RNA prior to reverse transcription in the same tube
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HL-dsDNase is inactivated without reducing quality or quantity of RNA
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Minimizes pipetting steps and suitable for high throughput experiments
gDNA removal kit uses a heat labile dsDNase to remove gDNA from RNA preps prior to reverse transcription.
The kit is based on a recombinant heat labile dsDNase which is irreversibly inactivated at low temperatures. This enables an inactivation step including heating to moderate temperatures only (58°C), which is gentle enough to preserve both quality and quantity of all present RNA. Reverse transcription can be performed in the same tube, minimizing pipetting steps and reducing hands-on time.
Fig 3. Simple protocol for removing genomic DNA from RNA
Fig 1. The gDNA removal kit used in RNA prep prior to RT-qPCR. The gDNA removal kit removes at least 50ng of gDNA in a 10µl reaction volume.
Fig 2. mRNA subjected to the gDNA removal kit protocol. All contaminating gDNA was removed while RNA was left unharmed. Compared to the untreated control, no reduction in quantified cDNA (SDHA) was detected.
Fig 3. Droplet digital PCR (ddPCR)-based DNA assay performed with 7 RNA preparations prior to RNA sequencing. Results courtesy of a clinical laboratory based in the UK obtained during the R&D of their RNA NGS workflow.
Fig 4. Droplet digital PCR (ddPCR)-based RNA assay performed with 7 cDNA preparations prior to RNA sequencing. Results courtesy of a clinical laboratory based in the UK obtained during the R&D of their RNA NGS workflow.
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Product Details
| Application Notes: | For the removal of gDNA from RNA prior to reverse transcription. |
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| Quality Control: | Tested for absence of RNase. |
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| Shipping: | Blue Ice |
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| Short Term Storage: | -20°C |
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| Long Term Storage: | -20°C |
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| Contents: | 10x reaction buffer
Heat-labile dsDNase |
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| Scientific Background: | Most techniques used for RNA isolation yield RNA with significant amounts of contaminating genomic DNA (gDNA), potentially resulting in false and inaccurate mRNA quantification. This is particularly a problem in the quantification of low-copy transcripts or small samples. The gDNA removal kit efficiently removes gDNA from RNA preps to levels below the detection limit of RT-qPCR. |
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| UniProt ID: | C9YSL6_PANBO |
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| Regulatory Status: | RUO - Research Use Only |
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Product Literature References
Limited predictive value of TFAM in mitochondrial biogenesis: N. Kozhukhar & M.F. Alexeyev; Mitochondrion
49, 156 (2019),
Application(s): To reduce mtDNA contamination prior to reverse transcription,
Abstract;
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