Optimized random hexamer and anchored oligo(dT) concentrations for generation of cDNA for qPCR
AMPIGENE® cDNA Synthesis Kit uses the latest developments in reverse transcriptase (RTase) technology and buffer chemistry for efficient and accurate cDNA synthesis.
The modified MMLV RTase is blended with RNase inhibitor to prevent degradation of RNA by contaminating RNase. The RTase is thermostable and extremely active.
An optimized buffer system contains oligo(dT), random hexamers, enhancers, dNTPs and MgCl2, allowing efficient and sensitive cDNA synthesis.
Characterization of Proteins Regulated by Androgen and Protein Kinase a Signaling in VCaP Prostate Cancer Cells: H.J. You, et al.; Biomedicines 9, 1404 (2021), Abstract;
Cryptotanshinone chemosensitivity potentiation by TW-37 in human oral cancer cell lines by targeting STAT3-Mcl-1 signaling: I. Yang, et al.; Cancer Cell Int. 20, 405 (2020), Abstract; Full Text
Loss of periodontal ligament fibroblasts by RIPK3-MLKL-mediated necroptosis in the progress of chronic periodontitis: B.S. Han, et al.; Sci. Rep. 9, 2902 (2019), Application(s): cDNA synthesis for PCR assay of HER2 and GADPH templates, Abstract; Full Text
Regulation of the translation activity of antigen-specific mRNA is responsible for antigen loss and tumor immune escape in a HER2-expressing tumor model: B.S. Han, et al.; Sci. Rep. 9, 2855 (2019), Abstract; Full Text
Oxidative stress, neurotoxicity, and metallothionein (MT) gene expression in juvenile rock fish Sebastes schlegelii under the different levels of dietary chromium (Cr6+) exposure: J.H. Kim & J.C. Kang; Ecotoxicol. Environ. Saf. 125, 78 (2016), Application(s): Purified RNA was subjected to reverse transcription to cDNA, Abstract;
The immune responses and expression of metallothionein (MT) gene and heat shock protein 70 (HSP 70) in juvenile rockfish, Sebastes schlegelii, exposed to waterborne arsenic (As3+): J.H. Kim, et al.; Environ. Toxicol. Pharmacol. 47, 136 (2016), Application(s): Specific gene expression - liver samples, Abstract;
The immune responses in juvenile rockfish, Sebastes schlegelii for the stress by the exposure to the dietary lead (II): J.H. Kim, et al.; Environ. Toxicol. Pharmacol. 46, 211 (2016), Application(s): Heat shock protein 70 gene expression, liver sample RNA, Abstract;
The toxic effects on the stress and immune responses in juvenile rockfish, Sebastes schlegelii exposed to hexavalent chromium: J.H. Kim, et al.; Environ. Toxicol. Pharmacol. 43, 128 (2016), Application(s): Purified RNA was subjected to reverse transcription to cDNA, Abstract;
Growth performance and immunological and antioxidant status of Chinese shrimp, Fennerpenaeus chinensis reared in bio-floc culture system using probiotics: M.S. Kim, et al.; Fish Shellfish Immunol. 47, 141 (2015), Application(s): RNA Reverse transcription, Abstract;
The toxic effects of ammonia exposure on antioxidant and immune responses in Rockfish, Sebastes schlegelii during thermal stress: S.H. Kim, et al.; Environ. Toxicol. Pharmacol. 40, 954 (2015), Application(s): Purified RNA was subjected to reverse transcription to cDNA, Abstract;
