Online Purchasing Account You are logged on as Guest. LoginRegister a New AccountShopping cart (Empty)
United States 

AMPIGENE® cDNA Synthesis Kit

Efficient and sensitive cDNA synthesis for downstream qPCR
 
ENZ-KIT106-0050 50 Reactions 250.00 USD
 
ENZ-KIT106-0200 200 Reactions 897.00 USD
Do you need bulk/larger quantities?
 
  • Unbiased, efficient, sensitive cDNA synthesis
  • Thermostable and extremely active RTase
  • Enhanced buffer system for efficient synthesis
  • Optimized random hexamer and anchored oligo(dT) concentrations for generation of cDNA for qPCR
AMPIGENE® cDNA Synthesis Kit uses the latest developments in reverse transcriptase (RTase) technology and buffer chemistry for efficient and accurate cDNA synthesis.
The modified MMLV RTase is blended with RNase inhibitor to prevent degradation of RNA by contaminating RNase. The RTase is thermostable and extremely active.
An optimized buffer system contains oligo(dT), random hexamers, enhancers, dNTPs and MgCl2, allowing efficient and sensitive cDNA synthesis.

Product Details

Application Notes:For cDNA synthesis for downstream qPCR reactions
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:-20°C
 
Contents:5x cDNA synthesis mix (anchored oligo (dT), random hexamers, 15mM MgCl2, 5mM dNTPs, enahncers, and stabilizers)
20x RTase (with RNase inhibitor)
 
Regulatory Status:RUO - Research Use Only
 

Product Literature References

Cryptotanshinone chemosensitivity potentiation by TW-37 in human oral cancer cell lines by targeting STAT3-Mcl-1 signaling: I. Yang, et al.; Cancer Cell Int. 20, 405 (2020), Abstract; Full Text
Loss of periodontal ligament fibroblasts by RIPK3-MLKL-mediated necroptosis in the progress of chronic periodontitis: B.S. Han, et al.; Sci. Rep. 9, 2902 (2019), Application(s): cDNA synthesis for PCR assay of HER2 and GADPH templates, Abstract; Full Text
Regulation of the translation activity of antigen-specific mRNA is responsible for antigen loss and tumor immune escape in a HER2-expressing tumor model: B.S. Han, et al.; Sci. Rep. 9, 2855 (2019), Abstract; Full Text
Oxidative stress, neurotoxicity, and metallothionein (MT) gene expression in juvenile rock fish Sebastes schlegelii under the different levels of dietary chromium (Cr6+) exposure: J.H. Kim & J.C. Kang; Ecotoxicol. Environ. Saf. 125, 78 (2016), Application(s): Purified RNA was subjected to reverse transcription to cDNA, Abstract;
The immune responses and expression of metallothionein (MT) gene and heat shock protein 70 (HSP 70) in juvenile rockfish, Sebastes schlegelii, exposed to waterborne arsenic (As3+): J.H. Kim, et al.; Environ. Toxicol. Pharmacol. 47, 136 (2016), Application(s): Specific gene expression - liver samples, Abstract;
The immune responses in juvenile rockfish, Sebastes schlegelii for the stress by the exposure to the dietary lead (II): J.H. Kim, et al.; Environ. Toxicol. Pharmacol. 46, 211 (2016), Application(s): Heat shock protein 70 gene expression, liver sample RNA, Abstract;
The toxic effects on the stress and immune responses in juvenile rockfish, Sebastes schlegelii exposed to hexavalent chromium: J.H. Kim, et al.; Environ. Toxicol. Pharmacol. 43, 128 (2016), Application(s): Purified RNA was subjected to reverse transcription to cDNA, Abstract;
Growth performance and immunological and antioxidant status of Chinese shrimp, Fennerpenaeus chinensis reared in bio-floc culture system using probiotics: M.S. Kim, et al.; Fish Shellfish Immunol. 47, 141 (2015), Application(s): RNA Reverse transcription, Abstract;
The toxic effects of ammonia exposure on antioxidant and immune responses in Rockfish, Sebastes schlegelii during thermal stress: S.H. Kim, et al.; Environ. Toxicol. Pharmacol. 40, 954 (2015), Application(s): Purified RNA was subjected to reverse transcription to cDNA, Abstract;

Related Products

AMPIGENE® 1-Step RT-PCR Kit 

Optimized and efficient cDNA synthesis and PCR in a single tube
PCR | Print as PDF
 
ENZ-KIT105-0050 50 Reactions 214.00 USD
 
ENZ-KIT105-0200 200 Reactions 753.00 USD
 
ENZ-KIT105-1000 1000 Reactions 3,093.00 USD
Do you need bulk/larger quantities?
 

AMPIGENE® Taq Mix 

Optimized standard PCR mix for enhanced speed, yield, and specificity
PCR | Print as PDF
 
ENZ-NUC100-0040 40 Reactions 42.00 USD
 
ENZ-NUC100-0200 200 Reactions 120.00 USD
 
ENZ-NUC100-1000 1000 Reactions 478.00 USD
Do you need bulk/larger quantities?
 

AMPIGENE® HS Taq Mix 

Increased sensitivity standard PCR mix for a broader range of samples, with enhanced speed, yield, and specificity
PCR | Print as PDF
 
ENZ-NUC101-0040 40 Reactions 53.00 USD
 
ENZ-NUC101-0200 200 Reactions 133.00 USD
 
ENZ-NUC101-1000 1000 Reactions 536.00 USD
Do you need bulk/larger quantities?
 

AMPIGENE® dNTP Mix  

Ultra-pure, stable, and suitable for a variety of PCR applications
≥99%, PCR | Print as PDF
 
ENZ-NUC102-0500 500 µl 53.00 USD
Do you need bulk/larger quantities?
 

AMPIGENE® qPCR Green Mix Lo-ROX 

Optimized dye-based qPCR
PCR | Print as PDF
 
ENZ-NUC103-0200 200 Reactions 141.00 USD
 
ENZ-NUC103-1000 1000 Reactions 570.00 USD
Do you need bulk/larger quantities?
 

AMPIGENE® qPCR Green Mix Hi-ROX 

Optimized dye-based qPCR
PCR | Print as PDF
 
ENZ-NUC104-0200 200 Reactions 141.00 USD
 
ENZ-NUC104-1000 1000 Reactions 572.00 USD
Do you need bulk/larger quantities?
 

AMPIGENE® qPCR Probe Mix Lo-ROX 

Optimized probe-based qPCR
PCR | Print as PDF
 
ENZ-NUC105-0200 200 Reactions 112.00 USD
 
ENZ-NUC105-1000 1000 Reactions 453.00 USD
Do you need bulk/larger quantities?
 

AMPIGENE® qPCR Probe Mix Hi-ROX 

Optimized probe-based qPCR
PCR | Print as PDF
 
ENZ-NUC106-0200 200 Reactions 112.00 USD
 
ENZ-NUC106-1000 1000 Reactions 453.00 USD
Do you need bulk/larger quantities?
 

AMPIGENE® qPCR Probe Mix No-ROX 

Optimized probe-based qPCR
PCR | Print as PDF
 
ENZ-NUC107-0200 200 Reactions 112.00 USD
 
ENZ-NUC107-1000 1000 Reactions 453.00 USD
Do you need bulk/larger quantities?
 

AMPIGENE® qPCR 1-Step Green Kit Lo-ROX 

Optimized and efficient cDNA synthesis and dye-based qPCR in a single tube
PCR | Print as PDF
 
ENZ-NUC108-0200 200 Reactions 298.00 USD
 
ENZ-NUC108-1000 1000 Reactions 1,193.00 USD
Do you need bulk/larger quantities?
 

AMPIGENE® qPCR 1-Step Green Kit Hi-ROX 

Optimized and efficient cDNA synthesis and dye-based qPCR in a single tube
PCR | Print as PDF
 
ENZ-NUC109-0200 200 Reactions 298.00 USD
 
ENZ-NUC109-1000 1000 Reactions 1,193.00 USD
Do you need bulk/larger quantities?
 

AMPIGENE® qPCR 1-Step Probe Kit Lo-ROX 

Optimized and efficient cDNA synthesis and probe-based qPCR in a single tube
PCR | Print as PDF
 
ENZ-NUC110-0200 200 Reactions 298.00 USD
 
ENZ-NUC110-1000 1000 Reactions 1,193.00 USD
Do you need bulk/larger quantities?
 

AMPIGENE® qPCR 1-Step Probe Kit Hi-ROX 

Optimized and efficient cDNA synthesis and probe-based qPCR in a single tube
PCR | Print as PDF
 
ENZ-NUC111-0200 200 Reactions 298.00 USD
 
ENZ-NUC111-1000 1000 Reactions 1,193.00 USD
Do you need bulk/larger quantities?
 

AMPIGENE® qPCR 1-Step Probe Kit No-ROX 

Optimized and efficient cDNA synthesis and probe-based qPCR in a single tube
PCR | Print as PDF
 
ENZ-NUC112-0200 200 Reactions 298.00 USD
 
ENZ-NUC112-1000 1000 Reactions 1,193.00 USD
Do you need bulk/larger quantities?
 

AMPIGENE® Taq DNA Polymerase 

Optimized standard PCR for enhanced speed, yield, and specificity
PCR | Print as PDF
 
ENZ-PRT100-0500 500 U 125.00 USD
Do you need bulk/larger quantities?
 

AMPIGENE® HS Taq DNA Polymerase 

Increased sensitivity standard PCR for a broader range of samples, with enhanced speed, yield, and specificity
PCR | Print as PDF
 
ENZ-PRT101-0500 500 U 268.00 USD
Do you need bulk/larger quantities?
 

Product Toolbox

PRODUCT RESOURCES

Datasheet
SDS
Certificate of Analysis
Manual

RELATED PRODUCTS

By target:
RNA
cDNA
By biological activity:
RNA Synthesis kit
cDNA Synthesis kit
By catalog section:

PRODUCT SUPPORT

FAQs
Technical Service
Customer Service

Related Literature

Brochures
Genomics & Molecular Biology
Genomics & Molecular Biology
Download as PDF

Brochures
PCR & qPCR Complete PCR Solutions
PCR & qPCR Complete PCR Solutions
Download as PDF

Product Flyers
Extraction Kit for Viral & Pathogen DNA and RNA
Extraction Kit for Viral & Pathogen DNA and RNA
Download as PDF

Product Flyers
AMPIGENE® PCR and qPCR
AMPIGENE® PCR and qPCR
Download as PDF

All new literature pieces