AMPINEXT™ Post-Bisulfite DNA Library Preparation Kit (Illumina)

A complete set of optimized reagents to prepare a DNA library, after successful bisulfite conversion, for various Illumina platform-based bisulfite sequencing (bisulfite-seq) assays.



ENZ-GEN512-0012	12 Reactions	679.00 USD

ENZ-GEN512-0024	24 Reactions	1,107.00 USD
Do you need bulk/larger quantities?

Allows bisulfite-converted DNA to be used directly for ligation.
Fast 5-hour procedure, from input starting material to library amplification.
Gel-free size selection/purification
High sensitivty and efficiency -- direct ligation of adapter to bisulfite-converted DNA fragments.
Comprehensive set of components to accommodate each step of DNA library preparation.
Ultra HiFi amplification enables achievement of reproducibly high yields of DNA libraries.

The AMPINEXT™ Post-Bisulfite DNA Library Preparation Kit is a complete set of optimized reagents to prepare a DNA library -- after successful bisulfite conversion -- for various Illumina platform-based bisulfite sequencing (bisulfite-seq) assays, such as whole genome bisulfite sequencing (WGBS), oxidative bisulfite sequencing (oxBs-seq), reduced representation bisulfite sequencing (RRBS), and other bisulfite-based next generation sequencing applications. The optimized protocol and components of the kit allow both non-barcoded (singleplexed) and barcoded (multiplexed) DNA libraries to be quickly constructed using sub-nanogram input concentrations of DNA since the DNA is first bisulfite-converted and then used for library preparation.

Figure 1. Schematic Procedure for using the AMPINEXT Post-Bisulfite DNA Library Preparation Kit (Illumina) Schematic

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Product Details

Assay Time:	5 hours

Application Notes:	A complete set of optimized reagents to prepare a DNA library, after successful bisulfite conversion, for various Illumina platform-based bisulfite sequencing (bisulfite-seq) assays.

Use/Stability:	Upon receipt, store the following components at -20°C immediately: 5X Conversion Buffer, Conversion Enzyme Mix, Conversion Primer, 10X End Repair Buffer, End Repair Enzyme Mix,10X dA-Tailing Buffer, Klenow Fragment (3’-5’ exo^-), 2X Ligation Buffer, T4 DNA Ligase, Adaptors, 2X HiFi PCR Master Mix, Primer U, Primer I, and Elution Buffer. Store the following components at 4°C: MQ Binding Beads. Store all other components at room temperature.

Shipping:	Blue Ice

Long Term Storage:	Ambient

Contents:	5X Conversion Buffer Conversion Enzyme Mix Conversion Primer 10X End Repair Buffer End Repair Enzyme Mix 10X dA-Tailing Buffer Klenow Fragment (3’-5’ exo^-) 2X Ligation Buffer T4 DNA Ligase Adaptors (50 µM) MQ Binding Beads 2X HiFi PCR Master Mix Primer U (10 µM) Primer I (10 µM) Elution Buffer

Regulatory Status:	RUO - Research Use Only