- High sensitivity and efficiency: Use for non-barcoded (singleplexed) and barcoded (multiplexed) library preparation.
- Easy and streamlined procedure: The entire procedure can be finished in 6 hours.
- Complete conversion: Converts unmethylated cytosine into uracil at a level greater than 99.9%.
- Extremely convenient: The kit contains all the required components for each step of the RNA library preparation process.
- Minimal bias: Ultra HiFi amplification.
- Broad sample suitability: Starting materials can be total RNA isolated from various tissue/cell samples.
The AMPINEXT™ 5-mC RNA Bisulfite-Seq Easy Kit (Illumina) is a complete set of optimized reagents designed for easily carrying out RNA bisulfite conversion, followed by a "post-bisulfite" library preparation process for Illumina platform-based bisulfite sequencing, all in one kit. Intended applications include whole transcriptome RNA bisulfite sequencing and various other RNA bisulfite-based next generation sequencing techniques for 5-methylcytosine (5-mC) based RNA methylation analysis. The optimized protocol and components of the kit allow the RNA to be bisulfite converted and fragmented simultaneously, followed by quick non-barcoded (singleplexed) and barcoded (multiplexed) library construction using low-nanogram quantities of bisulfite converted RNA.
Figure 1. Schematic Procedure for using the AMPINEXT 5-mC RNA Bisulfite-Seq Easy Kit (Illumina)
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Product Details
| Assay Time: | 6 hours |
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| Application Notes: | A complete set of optimized reagents designed for easily carrying out RNA bisulfite conversion, followed by a "post-bisulfite" library preparation process for Illumina platform-based bisulfite sequencing, all in one kit. |
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| Use/Stability: | Upon receipt: (1) Store the following components at -20°C immediately: 5X Reaction Buffer, Reaction Enzyme Mix, Adaptor-A, Adaptor-B, RNA Digestion Buffer, RNA Digestion Enzyme, 2X HiFi PCR Master Mix, Primer U, Primer I, and Elution Buffer. (2) Store the following components at 4°C: MQ Binding Beads. (3) Store all other components at room temperature away from light. |
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| Shipping: | Blue Ice |
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| Contents: | Conversion Buffer
Conversion Powder
NA Binding Solution
F-Spin Column
F-Collection Tube
Desulphonation Solution
5X Reaction Buffer
Reaction Enzyme Mix
Adaptor-A (10 µM)
Adaptor-B (10 µM)
RNA Digestion Buffer
RNA Digestion Enzyme
MQ Binding Beads
2X HiFi PCR Master Mix
Primer U (10 µM)
Primer I (10 µM)
Elution Buffer |
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| Regulatory Status: | RUO - Research Use Only |
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