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AMPINEXT™ 5-mC RNA Bisulfite-Seq Easy Kit (Illumina)

A complete set of optimized reagents designed for easily carrying out RNA bisulfite conversion, followed by a "post-bisulfite" library preparation process for Illumina platform-based bisulfite sequencing, all in one kit.
 
ENZ-GEN511-0012 12 Reactions 598.00 USD
 
ENZ-GEN511-0024 24 Reactions 1,152.00 USD
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  • High sensitivity and efficiency: Use for non-barcoded (singleplexed) and barcoded (multiplexed) library preparation.
  • Easy and streamlined procedure: The entire procedure can be finished in 6 hours.
  • Complete conversion: Converts unmethylated cytosine into uracil at a level greater than 99.9%.
  • Extremely convenient: The kit contains all the required components for each step of the RNA library preparation process.
  • Minimal bias: Ultra HiFi amplification.
  • Broad sample suitability: Starting materials can be total RNA isolated from various tissue/cell samples.
The AMPINEXT™ 5-mC RNA Bisulfite-Seq Easy Kit (Illumina) is a complete set of optimized reagents designed for easily carrying out RNA bisulfite conversion, followed by a "post-bisulfite" library preparation process for Illumina platform-based bisulfite sequencing, all in one kit. Intended applications include whole transcriptome RNA bisulfite sequencing and various other RNA bisulfite-based next generation sequencing techniques for 5-methylcytosine (5-mC) based RNA methylation analysis. The optimized protocol and components of the kit allow the RNA to be bisulfite converted and fragmented simultaneously, followed by quick non-barcoded (singleplexed) and barcoded (multiplexed) library construction using low-nanogram quantities of bisulfite converted RNA.
AMPINEXT 5-mC RNA Bisulfite-Seq Easy Kit (Illumina) Schematic
Figure 1. Schematic Procedure for using the AMPINEXT 5-mC RNA Bisulfite-Seq Easy Kit (Illumina)
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AMPINEXT 5-mC RNA Bisulfite-Seq Easy Kit (Illumina) Schematic

Product Specification

Assay Time:6 hours
 
Application Notes:A complete set of optimized reagents designed for easily carrying out RNA bisulfite conversion, followed by a "post-bisulfite" library preparation process for Illumina platform-based bisulfite sequencing, all in one kit.
 
Use/Stability:Upon receipt: (1) Store the following components at -20°C immediately:  5X Reaction Buffer, Reaction Enzyme Mix, Adaptor-A, Adaptor-B, RNA Digestion Buffer, RNA Digestion Enzyme, 2X HiFi PCR Master Mix, Primer U, Primer I, and Elution Buffer. (2) Store the following components at 4°C: MQ Binding Beads. (3) Store all other components at room temperature away from light.
 
Shipping:Shipped on Blue Ice
 
Contents:Conversion Buffer
Conversion Powder
NA Binding Solution
F-Spin Column
F-Collection Tube
Desulphonation Solution
5X Reaction Buffer
Reaction Enzyme Mix
Adaptor-A (10 µM)
Adaptor-B (10 µM)
RNA Digestion Buffer
RNA Digestion Enzyme
MQ Binding Beads
2X HiFi PCR Master Mix
Primer U (10 µM)
Primer I (10 µM)
Elution Buffer
 
Regulatory Status:RUO - Research Use Only
 

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