- Innovative method - Allows for simultaneous bisulfite conversion and size-appropriate DNA fragmentation. .
- Fast and streamlined procedure - Ready-to-use library DNA within 6 hours and 30 minutes.
- Complete conversion - Converts unmethylated cytosine into uracil (>99%).
- High sensitivity and efficiency - Innovative adaptor ligation of bisulfite DNA eliminates loss of fragments and selection bias.
- Extremely convenient - The kit contains all required components for each step of DNA library preparation,
- Minimal bias - Ultra HiFi amplification.
- Broad sample suitability - Starting materials can be genomic DNA isolated from various tissue/cell samples.
The AMPINEXT™ High-Sensitivity Bisulfite-Seq Kit (Illumina) is designed to bisulfite-convert DNA and prepare an Illumina-based library for bisulfite sequencing, all in one kit. Intended applications include whole genome (WGBS), oxidative (oxBS-seq), reduced representation (RRBS), and other bisulfite-next generation sequencing. The optimized protocol and components of the kit allow subnanogram amounts of DNA to be bisulfite converted and fragmented simultaneously followed by quick non-barcoded (singleplexed) and barcoded (multiplexed) library construction in less than 7 hours.
Figure 1. Schematic Procedure for using the AMPINEXT High-Sensitivity Bisulfite-Seq Kit (Illumina)
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Product Details
| Assay Time: | <7 hours |
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| Application Notes: | Designed to bisulfite-convert DNA and prepare an Illumina-based library for bisulfite sequencing, all in one kit. |
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| Use/Stability: | Upon receipt: (1) Store the following components at -20°C immediately: 5X Reaction Buffer, Reaction Enzyme Mix, Adaptor-F, Adaptor-S, 2X HiFi PCR Master Mix, Primer U, Primer I, and Elution Buffer. (2) Store the following components at 4°C: MQ Binding Beads. (3) Store all other components at room temperature away from light. |
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| Shipping: | Blue Ice |
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| Contents: | Modification Buffer
Modification Powder
DNA Binding Solution
Desulphonation Solution
Elution Solution
F-Spin Column
F-Collection Tube
5X Reaction Buffer
Reaction Enzyme Mix
Adaptor-F (10 µM)
Adaptor-S (10 µM)
MQ Binding Beads
2X HiFi PCR Master Mix
Primer U (10 µM)
Primer I (10 µM)
Elution Buffer |
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| Regulatory Status: | RUO - Research Use Only |
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