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AMPINEXT™ High-Sensitivity Bisulfite-Seq Kit (Illumina)

Designed to bisulfite-convert DNA and prepare an Illumina-based library for bisulfite sequencing, all in one kit
ENZ-GEN508-0012 12 Reactions 765.00 USD
ENZ-GEN508-0024 24 Reactions 1,371.00 USD
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  • Innovative method - Allows for simultaneous bisulfite conversion and size-appropriate DNA fragmentation. .
  • Fast and streamlined procedure - Ready-to-use library DNA within 6 hours and 30 minutes.
  • Complete conversion -  Converts unmethylated cytosine into uracil (>99%).
  • High sensitivity and efficiency - Innovative adaptor ligation of bisulfite DNA eliminates loss of fragments and selection bias.
  • Extremely convenient - The kit contains all required components for each step of DNA library preparation,
  • Minimal bias - Ultra HiFi amplification.
  • Broad sample suitability - Starting materials can be genomic DNA isolated from various tissue/cell samples.
The AMPINEXT™ High-Sensitivity Bisulfite-Seq Kit (Illumina) is designed to bisulfite-convert DNA and prepare an Illumina-based library for bisulfite sequencing, all in one kit. Intended applications include whole genome (WGBS), oxidative (oxBS-seq), reduced representation (RRBS), and other bisulfite-next generation sequencing. The optimized protocol and components of the kit allow subnanogram amounts of DNA to be  bisulfite converted and fragmented simultaneously followed by quick non-barcoded (singleplexed) and barcoded (multiplexed) library construction in less than 7 hours.
AMPINEXT High-Sensitivity Bisulfite-Seq Kit (Illumina) schematic
Figure 1. Schematic Procedure for using the AMPINEXT High-Sensitivity Bisulfite-Seq Kit (Illumina)
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AMPINEXT High-Sensitivity Bisulfite-Seq Kit (Illumina) schematic

Product Details

Assay Time:<7 hours
Application Notes:Designed to bisulfite-convert DNA and prepare an Illumina-based library for bisulfite sequencing, all in one kit.
Use/Stability:Upon receipt: (1) Store the following components at -20°C immediately: 5X Reaction Buffer, Reaction Enzyme Mix, Adaptor-F, Adaptor-S, 2X HiFi PCR Master Mix, Primer U, Primer I, and Elution Buffer. (2) Store the following components at 4°C: MQ Binding Beads. (3) Store all other components at room temperature away from light.
Shipping:Blue Ice
Contents:Modification Buffer
Modification Powder
DNA Binding Solution
Desulphonation Solution
Elution Solution
F-Spin Column
F-Collection Tube
5X Reaction Buffer
Reaction Enzyme Mix
Adaptor-F (10 µM)
Adaptor-S (10 µM)
MQ Binding Beads
2X HiFi PCR Master Mix
Primer U (10 µM)
Primer I (10 µM)
Elution Buffer
Regulatory Status:RUO - Research Use Only

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