- Fast - Entire procedure can be finished in 20 minutes with consistent isolation conditions.
- Effective - High efficiency of DNA isolation from blood leukocytes or cultured mammalian cells.
- Reliable - Our specially designed F-Spin Columns allow for DNA to be conveniently recovered.
- Safe - Use of non-toxic reagents and no phenol chloroform.
The AMPIXTRACT® Blood and Cultured Cell DNA Extraction Kit is a complete set of essential components designed for rapid isolation of pure genomic DNA. The researcher can quickly and efficiently isolate DNA from blood leukocytes and cultured mammalian cells. The entire procedure can be completed within 20 minutes.
Figure 1. Schematic Procedure for using the AMPIXTRACT® Blood and Cultured Cell DNA Extraction Kit
Please mouse over
Product Details
Assay Time: | 20 minutes |
|
Application Notes: | For isolating DNA from blood leukocytes and cultured mammalian cells |
|
Use/Stability: | The kit can be stored at room temperature (15-22°C) for up to 6 months from the date of shipment, with the exception of CB3. Upon receipt, CB3 should be stored at -20°C, or stored at 4°C as soon as it is dissolved in CB2 (stable for up to 6 months). |
|
Shipping: | Ambient |
|
Contents: | CB1 (Suspending Buffer)
CB2 (DNA Digestion Solution)
CB3 (DNA Digestion Powder)
CB4 (DNA Capture Buffer)
CB5 (DNA Elution Solution)
F-Spin Column
F-Collection Tube
Product Manual |
|
Technical Info/Product Notes: | Input amount of blood leukocytes or cultured mammalian cells should be between 1 ng to 4 µg, with the optimal range between 10 ng and 1 µg. This kit can easily yield 1 ng to 4 µg of DNA in spin column format, which can be used for any molecular biology procedures such as PCR, restriction digestion, cloning and sequencing, etc. |
|
Protocol: | This kit simply applies our DNA isolation buffer to cell pellets. After treatment with the DNA digestion buffer, DNA is easily recovered in quantities of 10-20 µl by our specially designed F-Spin Column. DNA is ready for downstream applications. |
|
Regulatory Status: | RUO - Research Use Only |
|