DEEPSEE^® CDKN2A/CEN3/7/17 Quad Probe Assay

DEEPSEE^® CDKN2A/CEN3/7/17 Quad Probe Assay contains fluorescently labeled nucleic acid probes for use in in situ hybridization to detect the most frequently encountered chromosomal abnormalities in bladder cancer cells. This includes three chromosome enumeration probes targeted to specific alpha satellite regions within the centromeres of chromosomes 3, 7, and 17. These probes, namely CEN 3 (red), CEN 7 (green), and CEN 17 (aqua), allow for rapid determination of chromosomal aneuploidy that is commonly found in bladder cancer cells. The fourth probe, CDKN2A (gold), targets the 9p21 locus enabling the detection of deletions or alterations of the tumor suppressor gene CDKN2A, also known as p16 or p16INK4a. Loss of this tumor suppressor gene often occurs in a wide variety of human tumors including bladder carcinoma. Hybridization of a target DNA specimen with Enzo’s DEEPSEE^® CDKN2A/CEN3/7/17 Quad Probe produces bright and intense red, green, aqua and gold fluorescent signals, which enables the direct visualization of chromosomal aberrations in chromosome 3, 7, 17 and the 9p21 locus, respectively.

• The CDKN2A specific FISH probe is optimized to detect chromosome 9 at 9p21.3 on urinary bladder carcinoma cell lines from Homo sapiens
• The chromosome 3 specific centromere enumeration (CEN) FISH probe is optimized to detect alpha satellite sequences in the centromere of chromosome 3 at 3p11.1--3q11.1 on urinary bladder carcinoma cell lines from Homo sapiens
• The chromosome 7 specific centromere enumeration (CEN) FISH probe is optimized to detect alpha satellite sequences in the centromere of chromosome 7 at 7p11.1--7q11.1 on urinary bladder carcinoma cell lines from Homo sapiens
• The chromosome 17 specific centromere enumeration (CEN) FISH probe is optimized to detect alpha satellite sequences in the centromere of chromosome 17 at 17p11.1--17q11.1 on urinary bladder carcinoma cell lines from Homo sapiens