| ENZ-GEN210-0500 | 500 µl | Inquire for pricing | |||
| Do you need bulk/larger quantities? | |||||
| Applications: | qPCR |
| Application Notes: | The AMPIPROBE® UP/Mg/Mh Extraction Controls is compatible with manual and automated DNA extraction methods as well as any properly calibrated qPCR thermal cycler capable to detect fluorescence decay. It has been validated for use on the QIAGEN QIAsymphony SP and Rotor-Gene Q. |
| Species reactivity: | Ureaplasma spp., Mycoplasma genitalium, Mycoplasma hominis |
| Use/Stability: | All components are stable at -20°C until the kit's expiration date. |
| Shipping: | Dry Ice |
| Short Term Storage: | +4°C |
| Long Term Storage: | -20°C |
| Contents: | Negative Extraction Control UP/Mg/MH Positive Extraction Control |
| Scientific Background: | Ureaplasma and Mycoplasma, which belong to the same Mycoplasmataceae family and Mollicutes class, are the smallest self-replicating organisms and are characterized by their lack of a cell wall. These characteristics and limited biosynthetic capabilities contribute to the parasitic nature of Ureaplasma and Mycoplasma. The class of pathogens is present in healthy individuals but has been associated with many adverse conditions affecting the reproductive tract. Mycoplasma genitalium is associated with urethritis, cervical inflammation, and pelvic inflammatory disease. M. hominis is often present concurrently with Ureaplasma species and is associated with a variety of conditions ranging from pelvic inflammatory diseases, chorioamnionitis, postpartum endometritis bacterial vaginosis, arthritis, osteoarthritis, wound infections, and several conditions in neonates. Ureaplasma and Mycoplasma, especially in combination with other conditions such as bacterial vaginosis or cervical incompetence, have been associated with adverse pregnancy outcomes, such as chorioamnionitis, spontaneous preterm labor and preterm premature rupture of membranes. The AMPIPROBE® UMM Assay provides rapid and accurate results for the qualitative detection of Ureaplasma spp. (Ureaplasma parvum and Ureaplasma urealyticum), Mycoplasma genitalium, and Mycoplasma hominis DNA in a user-supplied sample of interest. |
| Protocol: | SETUP of DNA Extraction control The procedure described below is optimized for the QIAsymphony SP instrument (Qiagen) starting from an AMPTIMA® Multitest Swab Specimen collection tube. Once thawed at room temperature, the AMPIPROBE Extraction Control tubes may be prepared. Label two 2.0 mL sample tubes with the appropriate control type (PC, NC), and then add 1.0 mL of blank APTIMA media to each tube. Next, add 25 µL of each AMPIPROBE Extraction Control into their associated, labelled 2.0 mL tube. The extraction controls are now prepared and ready for use. Continue extraction procedure same as patient samples. PCR SETUP Please refer to the PCR SETUP protocol present in Product Manual AMPIPROBE® UP/Mg/Mh Assay Kit (Catalog #: ENZ-GEN209-0100, 100 Assays) and treat UP/Mg/Mh Positive Extraction Control and Negative Extraction controls as Samples. Note: The reagents required for the PCR Master Mix provided in the AMPIROBE® UP/Mg/Mh Assay kits will be sufficient to run also the UP/Mg/Mh Extraction Controls. |
| Regulatory Status: | RUO - Research Use Only |
AMPIGENE® qPCR 1-Step Probe Kit No-ROX |
||||||
| Optimized and efficient cDNA synthesis and probe-based qPCR in a single tube | ||||||
| PCR | Print as PDF | ||||||
| ENZ-NUC112-0200 | 200 Reactions | 319.00 USD |  |
|||
| ENZ-NUC112-1000 | 1000 Reactions | 1,277.00 USD | |
|||
| Do you need bulk/larger quantities? | ||||||
AMPIPROBE® HCV assay kit |
||||||
| Sensitive and unique qPCR assay for quantifying HCV viral load | ||||||
| qPCR | Print as PDF | ||||||
| ENZ-GEN200-0100 | 100 tests | Inquire for pricing | ||||
| Do you need bulk/larger quantities? | ||||||
AMPIPROBE® Candida 1 Assay Kit |
||||||
| Sensitive and specific qPCR assay for the detection of Candida species | ||||||
| qPCR | Print as PDF | ||||||
| ENZ-GEN202-0100 | 100 tests | Inquire for pricing | ||||
| Do you need bulk/larger quantities? | ||||||
AMPIPROBE® Candida 2 Assay Kit |
||||||
| Sensitive and specific qPCR assay for the detection of Candida species | ||||||
| qPCR | Print as PDF | ||||||
| ENZ-GEN203-0100 | 100 tests | Inquire for pricing | ||||
| Do you need bulk/larger quantities? | ||||||
AMPIPROBE® Bacterial Vaginosis Assay Kit |
||||||
| Sensitive, specific and semi-quantitative qPCR assay for the detection of Bacterial Vaginosis | ||||||
| qPCR | Print as PDF | ||||||
| ENZ-GEN205-0100 | 100 tests | Inquire for pricing | ||||
| Do you need bulk/larger quantities? | ||||||
AMPIPROBE® NG/CT/TV Assay Kit |
||||||
| Sensitive and specific qPCR assay for the detection of NG/CT/TV | ||||||
| qPCR | Print as PDF | ||||||
| ENZ-GEN207-0100 | 100 tests | Inquire for pricing | ||||
| Do you need bulk/larger quantities? | ||||||
AMPIPROBE® Ureaplasma spp. / M. genitalium / M. hominis Assay Kit |
||||||
| Sensitive and specific qPCR assay for the detection of Ureaplasma spp. / M. genitalium / M. hominis | ||||||
| qPCR | Print as PDF | ||||||
| ENZ-GEN209-0100 | 100 tests | Inquire for pricing | ||||
| Do you need bulk/larger quantities? | ||||||
AMPIPROBE® Candida Extraction Controls |
||||||
| The AMPIPROBE® Candida Extraction Controls are designed to ensure an efficient genomic DNA extraction process and detection of Candida albicans, Candida Glabrata, Candida krusei, Candida parapsilosis and Candida tropicalis. | ||||||
| qPCR | Print as PDF | ||||||
| ENZ-GEN204-0500 | 500 µl | Inquire for pricing | ||||
| Do you need bulk/larger quantities? | ||||||
AMPIPROBE® Bacterial Vaginosis Extraction Controls |
||||||
| The AMPIPROBE® Bacterial Vaginosis Extraction Controls are designed to ensure an efficient genomic DNA extraction process and detection of Atopobium vaginae, Gardnerella vaginalis, Lactobacillus spp., Megasphaera spp., and BVAB2. | ||||||
| qPCR | Print as PDF | ||||||
| ENZ-GEN206-0500 | 500 µl | Inquire for pricing | ||||
| Do you need bulk/larger quantities? | ||||||
AMPIPROBE® NG/CT/TV Extraction Controls |
||||||
| The AMPIPROBE® NG/CT/TV Extraction Controls are designed to ensure an efficient genomic DNA extraction process and detection of Neisseria gonorrhoeae, Chlamydia trachomatis and Trichomonas vaginalis. | ||||||
| qPCR | Print as PDF | ||||||
| ENZ-GEN208-0500 | 500 µl | Inquire for pricing | ||||
| Do you need bulk/larger quantities? | ||||||
 |
|
| Download as PDF | |
 |
 |
| Download as PDF | |
 |
|
| Download as PDF | |
