| ENZ-GEN208-0500 | 500 µl | Inquire for pricing | |||
| Do you need bulk/larger quantities? | |||||
| Applications: | qPCR |
| Application Notes: | The AMPIPROBE® NG/CT/TV Extraction Controls is compatible with manual and automated DNA extraction methods as well as any properly calibrated qPCR thermal cycler capable to detect fluorescence decay. It has been validated for use on the QIAGEN QIAsymphony SP and Rotor-Gene Q. |
| Species reactivity: | Neisseria gonorrhoeae, Chlamydia trachomatis, Trichomonas vaginalis |
| Use/Stability: | All components are stable at -20°C until the kit's expiration date. |
| Shipping: | Dry Ice |
| Short Term Storage: | +4°C |
| Long Term Storage: | -20°C |
| Contents: | Negative Extraction Control NG/CT/TV Positive Extraction Control |
| Scientific Background: | Sexually transmitted infections caused by N. gonorrhoeae, C. trachomatis, and T. vaginalis DNA are among the most curable venereal diseases, but can all lead to serious conditions such as pelvic inflammatory disease (PID) if left untreated. Over 100 million new cases of gonorrhoeae each year are caused by Neisseria gonorrhoeae, a gram negative bacteria. Gonorrhoeae infections can lead to severe complications including epididymitis in men and PID in women. Consequently, PID can result in involuntary infertility and ectopic pregnancy. In addition, N. gonorrhoeae can infect the eyes of newborns during birth which can potentially lead to blindness. Chlamydia trachomatis is also a gram negative bacterial pathogen that is the cause of sexually transmitted chlamydial infections with over 100 million cases each year. Infection with C. trachomatis can lead to PID, which consequently PID can result in ectopic pregnancy, infertility and chronic pelvic pain. Trichomonas vaginalis is a parasite that infects over 270 million patients worldwide each year, making it the most common sexually transmitted disease (STD) agent in the world. Left untreated, a persistent T. vaginalis infection can lead to PID and adverse outcomes of pregnancy. The current assay provides rapid and accurate results on the qualitative detection of N. gonorrhoeae, C. trachomatis, and T. vaginalis DNA in a user-supplied sample of interest. |
| Protocol: | SETUP of DNA Extraction control The procedure described below is optimized for the QIAsymphony SP instrument (Qiagen) starting from an AMPTIMA® Multitest Swab Specimen collection tube. Once thawed at room temperature, the AMPIPROBE Extraction Control tubes may be prepared. Label two 2.0 mL sample tubes with the appropriate control type (PC, NC), and then add 1.0 mL of blank APTIMA media to each tube. Next, add 25 µL of each AMPIPROBE Extraction Control into their associated, labelled 2.0 mL tube. The extraction controls are now prepared and ready for use. Continue extraction procedure same as patient samples. PCR SETUP Please refer to the PCR SETUP protocol present in Product Manual AMPIPROBE® NG/CT/TV Assay Kit (Catalog #: ENZ-GEN207-0100, 100 Assays) and treat NG/CT/TV Positive Extraction Control and Negative Extraction controls as Samples. Note: The reagents required for the PCR Master Mix provided in the AMPIROBE® NG/CT/TV Assay kits will be sufficient to run also the NG/CT/TV Extraction Controls. |
| Regulatory Status: | RUO - Research Use Only |
AMPIPROBE® HCV assay kit |
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| Sensitive and unique qPCR assay for quantifying HCV viral load | ||||||
| qPCR | Print as PDF | ||||||
| ENZ-GEN200-0100 | 100 tests | Inquire for pricing | ||||
| Do you need bulk/larger quantities? | ||||||
AMPIPROBE® Candida 1 Assay Kit |
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| Sensitive and specific qPCR assay for the detection of Candida species | ||||||
| qPCR | Print as PDF | ||||||
| ENZ-GEN202-0100 | 100 tests | Inquire for pricing | ||||
| Do you need bulk/larger quantities? | ||||||
AMPIPROBE® Candida 2 Assay Kit |
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| Sensitive and specific qPCR assay for the detection of Candida species | ||||||
| qPCR | Print as PDF | ||||||
| ENZ-GEN203-0100 | 100 tests | Inquire for pricing | ||||
| Do you need bulk/larger quantities? | ||||||
AMPIPROBE® Bacterial Vaginosis Assay Kit |
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| Sensitive, specific and semi-quantitative qPCR assay for the detection of Bacterial Vaginosis | ||||||
| qPCR | Print as PDF | ||||||
| ENZ-GEN205-0100 | 100 tests | Inquire for pricing | ||||
| Do you need bulk/larger quantities? | ||||||
AMPIPROBE® NG/CT/TV Assay Kit |
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| Sensitive and specific qPCR assay for the detection of NG/CT/TV | ||||||
| qPCR | Print as PDF | ||||||
| ENZ-GEN207-0100 | 100 tests | Inquire for pricing | ||||
| Do you need bulk/larger quantities? | ||||||
AMPIPROBE® Ureaplasma spp. / M. genitalium / M. hominis Assay Kit |
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| Sensitive and specific qPCR assay for the detection of Ureaplasma spp. / M. genitalium / M. hominis | ||||||
| qPCR | Print as PDF | ||||||
| ENZ-GEN209-0100 | 100 tests | Inquire for pricing | ||||
| Do you need bulk/larger quantities? | ||||||
AMPIGENE® qPCR 1-Step Probe Kit No-ROX |
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| Optimized and efficient cDNA synthesis and probe-based qPCR in a single tube | ||||||
| PCR | Print as PDF | ||||||
| ENZ-NUC112-0200 | 200 Reactions | 319.00 USD |  |
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| ENZ-NUC112-1000 | 1000 Reactions | 1,277.00 USD | |
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| Do you need bulk/larger quantities? | ||||||
AMPIPROBE® Candida Extraction Controls |
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| The AMPIPROBE® Candida Extraction Controls are designed to ensure an efficient genomic DNA extraction process and detection of Candida albicans, Candida Glabrata, Candida krusei, Candida parapsilosis and Candida tropicalis. | ||||||
| qPCR | Print as PDF | ||||||
| ENZ-GEN204-0500 | 500 µl | Inquire for pricing | ||||
| Do you need bulk/larger quantities? | ||||||
AMPIPROBE® Bacterial Vaginosis Extraction Controls |
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| The AMPIPROBE® Bacterial Vaginosis Extraction Controls are designed to ensure an efficient genomic DNA extraction process and detection of Atopobium vaginae, Gardnerella vaginalis, Lactobacillus spp., Megasphaera spp., and BVAB2. | ||||||
| qPCR | Print as PDF | ||||||
| ENZ-GEN206-0500 | 500 µl | Inquire for pricing | ||||
| Do you need bulk/larger quantities? | ||||||
AMPIPROBE® UP/Mg/Mh Extraction Controls |
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| The AMPIPROBE® UP/Mg/Mh Extraction Controls are designed to ensure an efficient genomic DNA extraction process and detection of Ureaplasma spp. (Ureaplasma parvum and Ureaplasma urealyticum), Mycoplasma genitalium and Mycoplasma hominis. | ||||||
| qPCR | Print as PDF | ||||||
| ENZ-GEN210-0500 | 500 µl | Inquire for pricing | ||||
| Do you need bulk/larger quantities? | ||||||
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