| ENZ-GEN204-0500 | 500 µl | Inquire for pricing | |||
| Do you need bulk/larger quantities? | |||||
| Applications: | qPCR |
| Application Notes: | The AMPIPROBE® Candida Extraction Controls is compatible with manual and automated DNA extraction methods as well as any properly calibrated qPCR thermal cycler capable to detect fluorescence decay. It has been validated for use on the QIAGEN QIAsymphony SP and Rotor-Gene Q. |
| Species reactivity: | Candida albicans, Candida glabrata, Candida krusei, Candida parapsilosis, Candida tropicalis |
| Use/Stability: | All components are stable at -20°C until the kit's expiration date. |
| Shipping: | Shipped on Dry Ice |
| Short Term Storage: | +4°C |
| Long Term Storage: | -20°C |
| Contents: | Negative Extraction control Candida Positive Extraction Control |
| Scientific Background: | Vulvovaginal candidiasis (VVC) is second, after bacterial vaginosis, among the many causes of vaginitis, and is diagnosed in up to 40% of women with vaginal complaints in the primary care setting. Approximately 70-75% of women will have at least one symptomatic Candida vulvovaginal infection during their lives. Accurate detection and differentiation of Candida species that cause VVC leads to a significant improvement in clinical management of women with vaginal complaints, especially in women with recurrent VVC (RVVC). The current assay provides rapid and accurate results for the detection of C. albicans, C. glabrata, and C. krusei DNA in a user-supplied sample of interest. |
| Protocol: | SETUP of DNA Extraction control The procedure described below is optimized for the QIAsymphony SP instrument (Qiagen) starting from an AMPTIMA® Multitest Swab Specimen collection tube. Once thawed at room temperature, the AMPIPROBE Extraction Control tubes may be prepared. Label two 2.0 mL sample tubes with the appropriate control type (PC, NC), and then add 1.0 mL of blank APTIMA media to each tube. Next, add 25 µL of each AMPIPROBE Extraction Control into their associated, labelled 2.0 mL tube. The extraction controls are now prepared and ready for use. Continue extraction procedure same as patient samples. PCR SETUP Please refer to the PCR SETUP protocol present in Product Manual AMPIPROBE® Candida 1 or Candida 2 Assay Kits (Catalog #: ENZ-GEN202-0100, 100 Assays or ENZ-GEN203-0100, 100 Assays) and treat the Candida Positive Extraction Control and Negative Extraction controls as Samples. Note: The reagents required for the PCR Master Mix provided in the AMPIROBE® Candida 1 or Candida 2 Assay kits will be sufficient to run also the Candida Extraction Controls. |
| Regulatory Status: | RUO - Research Use Only |
AMPIGENE® qPCR 1-Step Probe Kit No-ROX |
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| Optimized and efficient cDNA synthesis and probe-based qPCR in a single tube | ||||||
| PCR | Print as PDF | ||||||
| ENZ-NUC112-0200 | 200 Reactions | 319.00 USD |  |
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| ENZ-NUC112-1000 | 1000 Reactions | 1,277.00 USD | |
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AMPIPROBE® HCV assay kit |
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| Sensitive and unique qPCR assay for quantifying HCV viral load | ||||||
| qPCR | Print as PDF | ||||||
| ENZ-GEN200-0100 | 100 tests | Inquire for pricing | ||||
| Do you need bulk/larger quantities? | ||||||
AMPIPROBE® Candida 1 Assay Kit |
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| Sensitive and specific qPCR assay for the detection of Candida species | ||||||
| qPCR | Print as PDF | ||||||
| ENZ-GEN202-0100 | 100 tests | Inquire for pricing | ||||
| Do you need bulk/larger quantities? | ||||||
AMPIPROBE® Candida 2 Assay Kit |
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| Sensitive and specific qPCR assay for the detection of Candida species | ||||||
| qPCR | Print as PDF | ||||||
| ENZ-GEN203-0100 | 100 tests | Inquire for pricing | ||||
| Do you need bulk/larger quantities? | ||||||
AMPIPROBE® Bacterial Vaginosis Assay Kit |
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| Sensitive, specific and semi-quantitative qPCR assay for the detection of Bacterial Vaginosis | ||||||
| qPCR | Print as PDF | ||||||
| ENZ-GEN205-0100 | 100 tests | Inquire for pricing | ||||
| Do you need bulk/larger quantities? | ||||||
AMPIPROBE® NG/CT/TV Assay Kit |
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| Sensitive and specific qPCR assay for the detection of NG/CT/TV | ||||||
| qPCR | Print as PDF | ||||||
| ENZ-GEN207-0100 | 100 tests | Inquire for pricing | ||||
| Do you need bulk/larger quantities? | ||||||
AMPIPROBE® Ureaplasma spp. / M. genitalium / M. hominis Assay Kit |
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| Sensitive and specific qPCR assay for the detection of Ureaplasma spp. / M. genitalium / M. hominis | ||||||
| qPCR | Print as PDF | ||||||
| ENZ-GEN209-0100 | 100 tests | Inquire for pricing | ||||
| Do you need bulk/larger quantities? | ||||||
AMPIPROBE® Bacterial Vaginosis Extraction Controls |
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| The AMPIPROBE® Bacterial Vaginosis Extraction Controls are designed to ensure an efficient genomic DNA extraction process and detection of Atopobium vaginae, Gardnerella vaginalis, Lactobacillus spp., Megasphaera spp., and BVAB2. | ||||||
| qPCR | Print as PDF | ||||||
| ENZ-GEN206-0500 | 500 µl | Inquire for pricing | ||||
| Do you need bulk/larger quantities? | ||||||
AMPIPROBE® NG/CT/TV Extraction Controls |
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| The AMPIPROBE® NG/CT/TV Extraction Controls are designed to ensure an efficient genomic DNA extraction process and detection of Neisseria gonorrhoeae, Chlamydia trachomatis and Trichomonas vaginalis. | ||||||
| qPCR | Print as PDF | ||||||
| ENZ-GEN208-0500 | 500 µl | Inquire for pricing | ||||
| Do you need bulk/larger quantities? | ||||||
AMPIPROBE® UP/Mg/Mh Extraction Controls |
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| The AMPIPROBE® UP/Mg/Mh Extraction Controls are designed to ensure an efficient genomic DNA extraction process and detection of Ureaplasma spp. (Ureaplasma parvum and Ureaplasma urealyticum), Mycoplasma genitalium and Mycoplasma hominis. | ||||||
| qPCR | Print as PDF | ||||||
| ENZ-GEN210-0500 | 500 µl | Inquire for pricing | ||||
| Do you need bulk/larger quantities? | ||||||
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