Highly sensitive and specific assay
Low-cost alternative to other methods of Candida detection
Compatible with most open qPCR platforms
Smaller sample input that allows remaining extracted samples to be used in other tests

The AMPIPROBE Candida 2 Assay is a qualitative real-time PCR assay for the detection of C. parapsilosis and C. tropicalis genomic DNA. The nucleotide sequences of the PCR primers have been optimized to target sequences within species-specific regions of the Candida genome. The Candida primers have been designed using AMPIPROBE technology which incorporates reporter and quencher dyes into the primers. Target detection is based on fluorescence decay when successive rounds of amplification bring fluorophore and quencher-labeled primer pairs in close proximity resulting in FRET. Loss of fluorescence below a defined threshold in a particular channel indicates sample positivity for the corresponding Candida species.
The AMPIPROBE Candida 2 Primer Mix includes primers for a ubiquitously conserved human housekeeping gene (human β-globin). Successful amplification of the internal control serves as an indicator of sample adequacy, extraction efficiency and successful amplification in each individual sample.

Product Details

Sensitivity:	In a validation study approved by the New York State Department of Health, the AMPIPROBE^® Candida 2 Assay was determined to have the following percentage of Sensitivity: Candida parapsilosis - 94.70% Candida tropicalis - 100.10%

Applications:	qPCR

Application Notes:	The AMPIPROBE^® Candida 2 Assay is compatible with any properly calibrated qPCR thermal cycler capable to detect fluorescence decay. It has been validated for use on the QIAGEN Rotor-Gene Q

Species reactivity:	Candida parapsilosis, Candida tropicalis

Specificity:	In a validation study approved by the New York State Department of Health, the AMPIPROBE^® Candida 1 Assay was determined to have the following percentage of Specificity: Candida parapsilosis - 100.00% Candida tropicalis - 96.50%

Use/Stability:	All components are stable at -20°C until the kit's expiration date.

Shipping:	Dry Ice

Short Term Storage:	+4°C

Long Term Storage:	-20°C

Contents:	AMPIGENE^® HS Taq DNA Polymerase AMPIGENE^® dNTP Mix AMPIPROBE^® 5X Assay Buffer AMPIPROBE^® Candida 2 Primer Mix Candida 2 Positive PCR Control Negative PCR Control Nuclease-free Water

Scientific Background:	Vulvovaginal candidiasis (VVC) is second, after bacterial vaginosis, among the many causes of vaginitis, and is diagnosed in up to 40% of women with vaginal complaints in the primary care setting. Approximately 70-75% of women will have at least one symptomatic Candida vulvovaginal infection during their lives. Accurate detection and differentiation of Candida species that cause VVC leads to a significant improvement in clinical management of women with vaginal complaints, especially in women with recurrent VVC (RVVC). The current assay provides rapid and accurate results for the detection of C. albicans, C. glabrata, and C. krusei DNA in a user-supplied sample of interest.

Regulatory Status:	RUO - Research Use Only

General Literature References

Vulvovaginal candidosis: J.D. Sobel; Lancet 369, 1961 (2007), Abstract;

Evaluation of vaginal complaints: M.R. Anderson, et al.; JAMA 291, 1368 (2004), Abstract;

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