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Smooth muscle actin monoclonal antibody (CGA7)

ENZ-C34933 0.5 ml ~10 µg 304.00 USD
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Alternative size available: ENZ-30933 (6ml)

Product Details

Immunogen:Partially purified chicken gizzard actin.
Source:Purified from hybridoma tissue culture supernatant.
Species reactivity:Human
Chicken, Monkey, Rabbit
Specificity:Recognizes actin isotypes α and γ of smooth muscle cells. This antibody labels leiomyosarcomas and leiomyomas but does not react with other neoplasms such as carcinoma or lymphoma. Except for myoepithelium, it is nonreactive with other mesenchymal and all epithelial cells.
Crossreactivity:Does not cross-react with actin of skeletal or cardiac muscle cells.
Applications:IHC (PS), WB
Recommended Dilutions/Conditions:Immunohistochemistry (paraffin sections; optimal results with this antibody are obtained when the tissue to be stained is fixed in alcohol-based fixatives such as methacarn. Aldehyde-fixed (formalin, e.g.) specimens are not recommended for use with CGA7. Choose a good quality enzyme-linked anti-mouse IgG2b antibody for detection).
General suggested starting dilution is 1:20-1:50.
Suggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually for each application.
Purity Detail:Affinity purified.
Formulation:Liquid. In PBS containing 0.1% sodium azide and 1% BSA.
Handling:Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20°C.
Shipping:Blue Ice
Short Term Storage:+4°C
Long Term Storage:-20°C
Regulatory Status:RUO - Research Use Only

Product Literature References

Combinatorial Treatment with Apelin-13 Enhances the Therapeutic Efficacy of a Preconditioned Cell-Based Therapy for Peripheral Ischemia: M. Samura, et al.; Sci. Rep. 6, Article number 19379 (2016), Application(s): Immunohistochemistry, Abstract; Full Text
A smooth muscle-specific monoclonal antibody recognizes smooth muscle actin isozymes: A.M. Gown, et al.; J. Cell Biol. 100, 807 (1985), Abstract; Full Text
CGA-7 and HHF, two monoclonal antibodies that recognize muscle actin and react with adherent cells in human long-term bone marrow cultures: P. Charbord, et al.; Blood 66, 1138 (1985), Abstract; Full Text

Related Products

Smooth muscle actin monoclonal antibody (CGA7) (Ready-to-Use) 

Purified from hybridoma tissue culture supernatant., IHC (PS), WB | Print as PDF
ENZ-30933 6 ml ~6 µg 261.00 USD
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