Mono- and polyubiquitinylated conjugates recombinant monoclonal antibody (UBCJ2)



ENZ-ABS840-0100	100 µg	171.00 USD

ENZ-ABS840-0500	500 µg	684.00 USD
Do you need bulk/larger quantities?

Product Details

Clone:	UBCJ2

Host:	Mouse

Isotype:	IgG1κ

Immunogen:	Poly-ubiquitinylated lysozyme.

UniProt ID:	P0CG47 (UBB), P0CG48 (UBC)

Source:	Purified from tissue culture supernatant.

Species reactivity:	Species independent

Specificity:	Recognizes mono- and polyubiquitinylated protein conjugates in a wide range of species.

Applications:	ELISA, Flow Cytometry, ICC, WB

Recommended Dilutions/Conditions:	ELISA (1:1000) Immunocytochemistry (1:250) Flow cytometry (1:100) Western Blot (1:1000) Suggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually for each application.

Purity Detail:	Protein A affinity purified.

Formulation:	Liquid. In PBS containing 0.09% sodium azide.

Handling:	Store unopened vial at -20°C. Avoid freeze/thaw cycles.

Shipping:	Shipped on Blue Ice

Long Term Storage:	-20°C

Regulatory Status:	RUO - Research Use Only

Mono- and polyubiquitinylated conjugates monoclonal antibody (UBCJ2) (FITC conjugate) ICC

Immunocytochemistry (ICC) analysis of ubiquitinylated protein conjugates using FITC labeled mono- and polyubiquitinylated conjugates mAb (UBCJ2), (Prod. No. ENZ-ABS840F): HeLa cells, pre-treated with proteasome inhibitor MG-132 (Prod. No. BML-PI102, or ENZ-51035) at 5μM for 12 hours prior to analysis with antibody at 1:250 dilution. Panel A: isotype control (Prod. No. ADI-SAB-600FI); Panel B: untreated; Panel C: proteasome inhibitor-treated HeLa cells. Left: 20X and Right: 63X amplified.

Mono- and polyubiquitinylated conjugates monoclonal antibody (UBCJ2) (FITC conjugate) FACS

Flow cytometry analysis of 106 Jurkat cells stained using Mono- and polyubiquitinylated conjugates mAb (UBCJ2) (FITC conjugate), (Prod. No. ENZ-ABS840F), at a concentration of 10µg/ml, side-by-side comparison to Mono- and polyubiquitinylated conjugates mAb (FK2) (fluorescein labeled), (Prod. No. BML-PW1210).

Mono- and polyubiquitinylated conjugates monoclonal antibody (UBCJ2) (HRP conjugate) WB 2

Western Blot analysis of ENZ-ABS840HRP detecting ubiquitin chains: Lane 1: MW Marker, Lane 2: K48-linked, Lane 3: K63-linked.

Please mouse over

Product Literature References

In situ proximity labeling identifies Lewy pathology molecular interactions in the human brain: B.A. Killinger, et al.; PNAS 119, e2114405119 (2022), Abstract;

Analysis of proteostasis during aging with western blot of detergent-soluble and insoluble protein fractions: M. Rai, et al.; STAR Protoc. 2, 100628 (2021), Abstract;

Murine Irgm paralogs regulate non-redundant functions to execute host defense to Toxoplasma gondii: J. Dockterman, et al.; Infect. Immun. 89, e0020221 (2021), Abstract;

The protease SPRTN and SUMOylation coordinate DNA-protein crosslink repair to prevent genome instability: A. Ruggiano, et al.; Cell Rep. 37, 110080 (2021), Abstract;

Related Products

	Mono- and polyubiquitinylated conjugates recombinant monoclonal antibody (UBCJ2) (HRP conjugate)
	Purified from tissue culture supernatant., ELISA, WB \| Print as PDF

	ENZ-ABS840HRP-0025	25 µg	158.00 USD

	ENZ-ABS840HRP-0100	100 µg	441.00 USD
Do you need bulk/larger quantities?

	Mono- and polyubiquitinylated conjugates recombinant monoclonal antibody (UBCJ2) (FITC conjugate)
	Purified from tissue culture supernatant., Flow Cytometry, ICC \| Print as PDF

	ENZ-ABS840F-0025	25 µg	280.00 USD

	ENZ-ABS840F-0100	100 µg	772.00 USD
Do you need bulk/larger quantities?

	Polyubiquitinylated conjugates recombinant monoclonal antibody (UBQM)
	Purified from tissue culture supernatant, ELISA, WB \| Print as PDF

	ENZ-ABS842-0100	100 µg	200.00 USD

	ENZ-ABS842-0500	500 µg	788.00 USD
Do you need bulk/larger quantities?