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Mono- and polyubiquitinylated conjugates monoclonal antibody (UBCJ2)



ENZ-ABS840-0100	100 µg	163.00 USD

ENZ-ABS840-0500	500 µg	651.00 USD
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Replaces Prod. #: BML-PW8810

Product Specification

Clone:	UBCJ2

Host:	Mouse

Isotype:	IgG1κ

Immunogen:	Poly-ubiquitinylated lysozyme.

UniProt ID:	P0CG47 (UBB), P0CG48 (UBC)

Source:	Purified from tissue culture supernatant.

Species reactivity:	Species independent

Specificity:	Recognizes mono- and polyubiquitinylated protein conjugates in a wide range of species.

Applications:	ELISA, Flow Cytometry, ICC, WB

Recommended Dilutions/Conditions:	ELISA (1:1000) Immunocytochemistry (1:250) Flow cytometry (1:100) Western Blot (1:1000) Suggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually for each application.

Purity Detail:	Protein A affinity purified.

Formulation:	Liquid. In PBS containing 0.09% sodium azide.

Handling:	Store unopened vial at -20°C. Avoid freeze/thaw cycles.

Shipping:	Shipped on Blue Ice

Long Term Storage:	-20°C

Regulatory Status:	RUO - Research Use Only

Mono- and polyubiquitinylated conjugates monoclonal antibody (UBCJ2) (FITC conjugate) ICC

Immunocytochemistry (ICC) analysis of ubiquitinylated protein conjugates using FITC labeled mono- and polyubiquitinylated conjugates mAb (UBCJ2), (Prod. No. ENZ-ABS840F): HeLa cells, pre-treated with proteasome inhibitor MG-132 (Prod. No. BML-PI102, or ENZ-51035) at 5μM for 12 hours prior to analysis with antibody at 1:250 dilution. Panel A: isotype control (Prod. No. ADI-SAB-600FI); Panel B: untreated; Panel C: proteasome inhibitor-treated HeLa cells. Left: 20X and Right: 63X amplified.

Mono- and polyubiquitinylated conjugates monoclonal antibody (UBCJ2) (FITC conjugate) FACS

Flow cytometry analysis of 106 Jurkat cells stained using Mono- and polyubiquitinylated conjugates mAb (UBCJ2) (FITC conjugate), (Prod. No. ENZ-ABS840F), at a concentration of 10µg/ml, side-by-side comparison to Mono- and polyubiquitinylated conjugates mAb (FK2) (fluorescein labeled), (Prod. No. BML-PW1210).

Mono- and polyubiquitinylated conjugates monoclonal antibody (UBCJ2) (HRP conjugate) WB 2

Western Blot analysis of ENZ-ABS840HRP detecting ubiquitin chains: Lane 1: MW Marker, Lane 2: K48-linked, Lane 3: K63-linked.

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