Product Details
Clone: | UBCJ2 |
|
Host: | Mouse |
|
Isotype: | IgG1κ |
|
Immunogen: | Poly-ubiquitinylated lysozyme. |
|
UniProt ID: | P0CG47 (UBB), P0CG48 (UBC) |
|
Source: | Purified from tissue culture supernatant. |
|
Species reactivity: | Species independent
|
|
Specificity: | Recognizes mono- and polyubiquitinylated protein conjugates in a wide range of species. |
|
Applications: | ELISA, Flow Cytometry, ICC, WB
|
|
Recommended Dilutions/Conditions: | ELISA (1:1000)
Immunocytochemistry (1:250)
Flow cytometry (1:100)
Western Blot (1:1000)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application. |
|
Purity Detail: | Protein A affinity purified. |
|
Formulation: | Liquid. In PBS containing 0.09% sodium azide. |
|
Handling: | Store unopened vial at -20°C. Avoid freeze/thaw cycles. |
|
Shipping: | Shipped on Blue Ice |
|
Long Term Storage: | -20°C |
|
Regulatory Status: | RUO - Research Use Only |
|
Immunocytochemistry (ICC) analysis of ubiquitinylated protein conjugates using FITC labeled mono- and polyubiquitinylated conjugates mAb (UBCJ2), (Prod. No. ENZ-ABS840F): HeLa cells, pre-treated with proteasome inhibitor MG-132 (Prod. No. BML-PI102, or ENZ-51035) at 5μM for 12 hours prior to analysis with antibody at 1:250 dilution. Panel A: isotype control (Prod. No. ADI-SAB-600FI); Panel B: untreated; Panel C: proteasome inhibitor-treated HeLa cells. Left: 20X and Right: 63X amplified.
Flow cytometry analysis of 106 Jurkat cells stained using Mono- and polyubiquitinylated conjugates mAb (UBCJ2) (FITC conjugate), (Prod. No. ENZ-ABS840F), at a concentration of 10µg/ml, side-by-side comparison to Mono- and polyubiquitinylated conjugates mAb (FK2) (fluorescein labeled), (Prod. No. BML-PW1210).
Western Blot analysis of ENZ-ABS840HRP detecting ubiquitin chains: Lane 1: MW Marker, Lane 2: K48-linked, Lane 3: K63-linked.
Please mouse over
Product Literature References
In situ proximity labeling identifies Lewy pathology molecular interactions in the human brain: B.A. Killinger, et al.; PNAS
119, e2114405119 (2022),
Abstract;
Analysis of proteostasis during aging with western blot of detergent-soluble and insoluble protein fractions: M. Rai, et al.; STAR Protoc.
2, 100628 (2021),
Abstract;
Murine Irgm paralogs regulate non-redundant functions to execute host defense to Toxoplasma gondii: J. Dockterman, et al.; Infect. Immun.
89, e0020221 (2021),
Abstract;
The protease SPRTN and SUMOylation coordinate DNA-protein crosslink repair to prevent genome instability: A. Ruggiano, et al.; Cell Rep.
37, 110080 (2021),
Abstract;
Related Products