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Mono- and polyubiquitinylated conjugates monoclonal antibody (UBCJ2)

 
ENZ-ABS840-0100 100 µg 163.00 USD
 
ENZ-ABS840-0500 500 µg 651.00 USD
Do you need bulk/larger quantities?
 
Replaces Prod. #: BML-PW8810

Product Specification

Clone:UBCJ2
 
Host:Mouse
 
Isotype:IgG1κ
 
Immunogen:Poly-ubiquitinylated lysozyme.
 
UniProt ID:P0CG47 (UBB), P0CG48 (UBC)
 
Source:Purified from tissue culture supernatant.
 
Species reactivity:Species independent
 
Specificity:Recognizes mono- and polyubiquitinylated protein conjugates in a wide range of species.
 
Applications:ELISA, Flow Cytometry, ICC, WB
 
Recommended Dilutions/Conditions:ELISA (1:1000)
Immunocytochemistry (1:250)
Flow cytometry (1:100)
Western Blot (1:1000)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
Purity Detail:Protein A affinity purified.
 
Formulation:Liquid. In PBS containing 0.09% sodium azide.
 
Handling:Store unopened vial at -20°C.  Avoid freeze/thaw cycles.
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:-20°C
 
Regulatory Status:RUO - Research Use Only
 
Mono- and polyubiquitinylated conjugates monoclonal antibody (UBCJ2) (FITC conjugate) ICC
Immunocytochemistry (ICC) analysis of ubiquitinylated protein conjugates using FITC labeled mono- and polyubiquitinylated conjugates mAb (UBCJ2), (Prod. No. ENZ-ABS840F): HeLa cells, pre-treated with proteasome inhibitor MG-132 (Prod. No. BML-PI102, or ENZ-51035) at 5μM for 12 hours prior to analysis with antibody at 1:250 dilution. Panel A: isotype control (Prod. No. ADI-SAB-600FI); Panel B: untreated; Panel C: proteasome inhibitor-treated HeLa cells. Left: 20X and Right: 63X amplified.
Mono- and polyubiquitinylated conjugates monoclonal antibody (UBCJ2) (FITC conjugate) FACS
Flow cytometry analysis of 106 Jurkat cells stained using Mono- and polyubiquitinylated conjugates mAb (UBCJ2) (FITC conjugate), (Prod. No. ENZ-ABS840F), at a concentration of 10µg/ml, side-by-side comparison to Mono- and polyubiquitinylated conjugates mAb (FK2) (fluorescein labeled), (Prod. No. BML-PW1210).
Mono- and polyubiquitinylated conjugates monoclonal antibody (UBCJ2) (HRP conjugate) WB 2
Western Blot analysis of ENZ-ABS840HRP detecting ubiquitin chains: Lane 1: MW Marker, Lane 2: K48-linked, Lane 3: K63-linked.
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Mono- and polyubiquitinylated conjugates monoclonal antibody (UBCJ2) (FITC conjugate) ICC Mono- and polyubiquitinylated conjugates monoclonal antibody (UBCJ2) (FITC conjugate) FACS Mono- and polyubiquitinylated conjugates monoclonal antibody (UBCJ2) (HRP conjugate) WB 2

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Mono- and polyubiquitinylated conjugates monoclonal antibody (UBCJ2) (HRP conjugate) 

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Mono- and polyubiquitinylated conjugates monoclonal antibody (UBCJ2) (FITC conjugate) 

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