| Alternative Name: | NPM, Nucleolar phosphoprotein B23, Nucleolar protein NO38, Numatrin |
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| Clone: | 1902 |
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| Host: | Mouse |
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| Isotype: | IgG2bκ |
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| Immunogen: | Human nucleophosmin (aa 185-287). |
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| UniProt ID: | P06748 |
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| Species reactivity: | Human
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| Specificity: | Recognizes a 33kDa glycoprotein, identified as Nucleophosmin (NPM). |
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| Applications: | ELISA, IHC, WB
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| Application Notes: | The antibody may be a useful aid for classification of acute myeloid leukemia. |
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| Purity Detail: | Protein G affinity purified. |
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| Formulation: | Liquid. In 1X PBS containing 0.1 mg/ml BSA and 0.05% sodium azide. |
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| Handling: | Avoid freeze/thaw cycles. |
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| Shipping: | Blue Ice |
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| Long Term Storage: | -20°C |
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| Scientific Background: | Nucleophosmin (NPM) is predominantly localized in the nucleus of cells in most tissues. NPM is involved in ribosomal assembly and rRNA transport. It is an abundant protein that is highly phosphorylated by Cdc2 kinase during mitosis. This phosphoprotein moves between the nucleus and the cytoplasm. It is thought to be involved in several processes including regulation of the ARF/p53 pathway. A number of genes are fusion partners, in particular the anaplastic lymphoma kinase gene on chromosome 2. Mutations in exon 12 affecting the C-terminus of the protein are associated with an aberrant cytoplasmic location. Mutations in this gene are associated with acute myeloid leukemia. |
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| Regulatory Status: | RUO - Research Use Only |
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IHC staining of FFPE human colon carcinoma with Nucleophosmin antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
IHC staining of FFPE human skin with Nucleophosmin antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
Western blot testing of human A431 cell lysate with Nucleophosmin antibody.
