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Aldolase A monoclonal antibody (AT3F9)

 
ENZ-ABS760-0100 100 µl 390.00 USD
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Product Specification

Alternative Name:ALDOA, Fructose bisphosphate aldolase A, Muscle-type aldolase
 
Clone:AT3F9
 
Host:Mouse
 
Isotype:IgG2aκ
 
Immunogen:Recombinant human Aldolase A (aa 1-364) purified from E. coli.
 
UniProt ID:P04075
 
Species reactivity:Human
 
Applications:ELISA, Flow Cytometry, ICC, IF, WB
 
Recommended Dilutions/Conditions:Western Blot (1:1,000)
Flow Cytometry (1:200)
ICC/IF (1:200)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
Purity Detail:Protein A affinity purified
 
Formulation:Liquid. In PBS, pH 7.4, containing 0.02% sodium azide and 10% glycerol.
 
Handling:Avoid freeze/thaw cycles.
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:-20°C
 
Scientific Background:Aldolase A, a glycolytic enzyme, catalyzes the conversion of fructose 1, 6-bisphosphate to 3-phosphoglyceraldehyde. This ubiquitous enzyme is present as three different isozymes: aldolase A, aldolase B, and aldolase C. Research studies suggest that aldolase A expression potentially differentiates between nonneoplastic liver diseases and hepatocarcinoma. Aldolase A is found in the developing embryo and is produced in even greater amounts in adult muscle. Aldolase A expression is repressed in adult liver, kidney, and intestine and similar to aldolase C levels in brain and other nervous tissue.
 
Regulatory Status:RUO - Research Use Only
 
Aldolase A monoclonal antibody (AT3F9) FACS
Flow cytometry analysis of ALDOA in A549 cell line, staining at 2-5ug for 1x106 cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).
Aldolase A monoclonal antibody (AT3F9) IF
IF analysis of ALDOA in A549 cells, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human A549 antibody (1:200) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).
Aldolase A monoclonal antibody (AT3F9) SDS-PAGE
The recombinant human ALDOA and cell lysates (40µg) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ALDOA antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: Recombinant Human ALDOA Lane 2.: HeLa cell lysate Lane 3.: HepG2 cell lysate
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Aldolase A monoclonal antibody (AT3F9) FACS Aldolase A monoclonal antibody (AT3F9) IF Aldolase A monoclonal antibody (AT3F9) SDS-PAGE

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