| Alternative Name: | AKR1C1, AK1C1 |
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| Clone: | AT6D10 |
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| Host: | Mouse |
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| Isotype: | IgG1κ |
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| Immunogen: | Recombinant human AKR1C1 (aa 1-323) purified from E. coli. |
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| UniProt ID: | Q04828 |
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| Species reactivity: | Human
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| Applications: | ELISA, Flow Cytometry, ICC, IF, WB
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| Purity Detail: | Protein G affinity purified |
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| Formulation: | Liquid. In PBS, pH 7.4, containing 0.02% sodium azide and 10% glycerol. |
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| Handling: | Avoid freeze/thaw cycles. |
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| Shipping: | Blue Ice |
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| Long Term Storage: | -20°C |
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| Scientific Background: | The human aldo-keto reductases 1C1 and 1C3 (AKR1C1 and AKR1C3) have major roles in pre receptor regulation of progesterone action. They can both convert progesterone to the less potent efficiencies. AKR1C1 and AKR1C3 also act as 3-ketosteroid reductase, and as such they can convert the most potent androgen 5alpha-DHT into 3beta-androstanediol, which is an estrogen receptor beta ligand, and into the inactive androgen 3alpha-androstanediol, respectively. |
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| Regulatory Status: | RUO - Research Use Only |
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The Recombinant protein (50ng) and Cell lysates (40µg) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human AKR1C1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1: Recombinant Human AKR1C1, Lane 2: HepG2 cell lysate, Lane 3: Raji cell lysate.
Flow cytometry analysis of AKR1C1 in A431 cell line, staining at 2-5ug for 1x106 cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).
IF analysis of AKR1C1 in A431 cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human AKR1C1 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).
