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Hexokinase 3 monoclonal antibody (AT4C12)

ENZ-ABS757-0100 100 µl 477.00 USD
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Product Details

Alternative Name:HK3, Hexokinase type III, Hexokinase-C
Immunogen:Recombinant human Hexokinase 3 (aa 1-923) purified from E. coli.
UniProt ID:P52790
Species reactivity:Human
Applications:ELISA, WB
Recommended Dilutions/Conditions:Western Blot (1:2,000).
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
Purity Detail:Protein A affinity purified
Formulation:Liquid. In PBS, pH 7.4, containing 0.02% sodium azide and 10% glycerol.
Handling:Avoid freeze/thaw cycles.
Shipping:Blue Ice
Long Term Storage:-20°C
Scientific Background:Hexokinase is the first enzyme in the glycolytic pathway, catalyzing the transfer of a phosphoryl group from ATP to glucose to form glucose-6-phosphate and ADP. In mammals, four distinct enzymes - types 1 to 4 hexokinases - have been identified. The enzyme is found in most cells, but there is tissue specificity for the particular type of hexokinase. Hexokinase 3 lacks the hydrophobic N-terminal sequence critical for targeting to mitochondria. Hexpkinase 3 may have anabolic functions, providing H6P for glycogen or lipid synthesis.
Regulatory Status:RUO - Research Use Only
Hexokinase 3 monoclonal antibody (AT4C12) SDS-PAGE
The recombianat protein (20ng) of Hexokinase four isoform were resolved by SDS-PAGE, transferred to PVDF membrane and probed with Hexokinase 3 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1: Recombinant human Hexokinase 1, Lane 2: Recombinant human Hexokinase 2, Lane 3: Recombinant human Hexokinase 3, Lane 4: Recombinant human Hexokinase 4.
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Hexokinase 3 monoclonal antibody (AT4C12) SDS-PAGE

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