Alternative Name: | HK2, Muscle form hexokinase, Hexokinase type II, Hexokinase-B |
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Clone: | 1A7 |
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Host: | Mouse |
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Isotype: | IgG1κ |
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Immunogen: | Recombinant human Hexokinase 2 (aa 1-917) purified from E. coli. |
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UniProt ID: | P52789 |
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Species reactivity: | Human
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Applications: | ELISA, Flow Cytometry, ICC, IF, WB
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Recommended Dilutions/Conditions: | Western Blot (1:2,000). Suggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually for each application. |
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Purity Detail: | Protein G affinity purified |
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Formulation: | Liquid. In PBS, pH 7.4, containing 0.02% sodium azide and 10% glycerol. |
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Handling: | Avoid freeze/thaw cycles. |
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Shipping: | Blue Ice |
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Long Term Storage: | -20°C |
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Scientific Background: | Hexokinase is the first enzyme in the glycolytic pathway, catalyzing the transfer of a phosphoryl group from ATP to glucose to form glucose-6-phosphate and ADP. In mammals, four distinct enzymes (types 1 to 4 hexokinases) have been identified. These enzymes are found in most cells, but there is tissue specificity for the particular type of hexokinase. Hexokinase 2 is found in the skeletal muscle and includes hydrophobic N-terminal sequence capable of targeting the hexokinase to mitochondria. |
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Regulatory Status: | RUO - Research Use Only |
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The Recombinant Proteins (20ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Hexokinase I, II antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1: Hexokinase 1 recombinant protein, Lane 2: Hexokinase 2 recombinant protein, Lane 3: Hexokinase 3 recombinant protein, Lane 4: Hexokinase 4 recombinant protein
Flow cytometry analysis of Hexokinase 2 in Jurkat cell line, staining at 2-5µg for 1x106 cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).
IF analysis of Hexokinase 1,2 in Jurkat cell line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human Hexokinase 2 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).