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Hexokinase 2 monoclonal antibody (1A7)

 
ENZ-ABS756-0100 100 µl 390.00 USD
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Product Specification

Alternative Name:HK2, Muscle form hexokinase, Hexokinase type II, Hexokinase-B
 
Clone:1A7
 
Host:Mouse
 
Isotype:IgG1κ
 
Immunogen:Recombinant human Hexokinase 2 (aa 1-917) purified from E. coli.
 
UniProt ID:P52789
 
Species reactivity:Human
 
Applications:ELISA, Flow Cytometry, ICC, IF, WB
 
Recommended Dilutions/Conditions:Western Blot (1:2,000).
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
Purity Detail:Protein G affinity purified
 
Formulation:Liquid. In PBS, pH 7.4, containing 0.02% sodium azide and 10% glycerol.
 
Handling:Avoid freeze/thaw cycles.
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:-20°C
 
Scientific Background:Hexokinase is the first enzyme in the glycolytic pathway, catalyzing the transfer of a phosphoryl group from ATP to glucose to form glucose-6-phosphate and ADP. In mammals, four distinct enzymes (types 1 to 4 hexokinases) have been identified. These enzymes are found in most cells, but there is tissue specificity for the particular type of hexokinase. Hexokinase 2 is found in the skeletal muscle and includes hydrophobic N-terminal sequence capable of targeting the hexokinase to mitochondria.
 
Regulatory Status:RUO - Research Use Only
 
Hexokinase 2 monoclonal antibody (1A7) SDS-PAGE
The Recombinant Proteins (20ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Hexokinase I, II antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1: Hexokinase 1 recombinant protein, Lane 2: Hexokinase 2 recombinant protein, Lane 3: Hexokinase 3 recombinant protein, Lane 4: Hexokinase 4 recombinant protein
Hexokinase 2 monoclonal antibody (1A7) FACS
Flow cytometry analysis of Hexokinase 2 in Jurkat cell line, staining at 2-5µg for 1x106 cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).
Hexokinase 2 monoclonal antibody (1A7) IF
IF analysis of Hexokinase 1,2 in Jurkat cell line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human Hexokinase 2 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).
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Hexokinase 2 monoclonal antibody (1A7) SDS-PAGE Hexokinase 2 monoclonal antibody (1A7) FACS Hexokinase 2 monoclonal antibody (1A7) IF

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