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Hexokinase 1 monoclonal antibody (4D7)

 
ENZ-ABS755-0100 100 µl 390.00 USD
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Product Specification

Alternative Name:HK1, Brain form hexokinase, Hexokinase type I, Hexokinase-A
 
Clone:4D7
 
Host:Mouse
 
Isotype:IgG2aκ
 
Immunogen:Recombinant human Hexokinase 1 (aa 1-917) purified from E. coli.
 
UniProt ID:P19367
 
Species reactivity:Human
 
Applications:ELISA, ICC, IF, WB
 
Purity Detail:Protein G affinity purified
 
Formulation:Liquid. In PBS, pH 7.4, containing 0.02% sodium azide and 10% glycerol.
 
Handling:Avoid freeze/thaw cycles.
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:-20°C
 
Scientific Background:Hexokinase is the first enzyme in the glycolytic pathway, catalyzing the transfer of a phosphoryl group from ATP to glucose to form glucose-6-phosphate and ADP. In mammals, four distinct enzymes - types 1 to 4 hexokinases - have been identified. The enzyme is found in most cells, but there is tissue specificity for the particular type of hexokinase. Hexokinase 1 is found in the adipose tissue and liver and encodes a ubiquitous form of hexokinase which localizes to the outer membrane of mitochondria. Mutations in this hexokinase1 have been associated with hemolytic anemia due to hexokinase deficiency.
 
Regulatory Status:RUO - Research Use Only
 
Hexokinase 1 monoclonal antibody (4D7) SDS-PAGE
The Recombinant Proteins (20ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Hexokinase antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1: Hexokinase 1 recombinant protein, Lane 2: Hexokinase 2 recombinant protein, Lane 3: Hexokinase 3 recombinant protein, Lane 4: Hexokinase 4 recombinant protein.
Hexokinase 1 monoclonal antibody (4D7) IF
IF analysis of Hexokinase in HeLa cells. The cell was stained with ENZ-ABS755 (1:100). Thesecondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).
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Hexokinase 1 monoclonal antibody (4D7) SDS-PAGE Hexokinase 1 monoclonal antibody (4D7) IF

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