| Alternative Name: | BCL2L4, BCL2-associated X protein |
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| Clone: | 6A7 |
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| Host: | Mouse |
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| Isotype: | IgG1κ |
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| Immunogen: | KLH conjugated to peptides corresponding to amino acids 3-16 of murine Bax (CGSGEQLGSGGPTSS), amino acids 3-16 of human Bax (CGSGEQPRGGGPTSS), and amino acids 12-24 shared by Bax from both species (CGPTSSEQIMKTGA). |
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| UniProt ID: | Q07812 |
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| Species reactivity: | Human, Mouse, Rat
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| Specificity: | Recognizes the active monomeric form of Bax. |
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| Crossreactivity: | In the presence of nonionic detergent, the antibody binds the monomeric form of Bax, but not Bax complexed with either Bcl-XL or Bcl-2. Only recognizes conformationally changed active Bax. |
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| Applications: | IHC, IP, WB
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| Purity Detail: | Protein A affinity purified. |
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| Formulation: | Liquid. In PBS with 0.02% Proclin 300. |
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| Handling: | After opening aliquot contents and freeze at -20°C. Avoid freeze/thaw cycles. |
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| Shipping: | Blue Ice |
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| Long Term Storage: | -20°C |
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| Scientific Background: | Bax and Bak operate in both the endoplasmatic reticulum (ER) and mitochondria as an essential gateway for selected apoptotic signals. A role for Bcl-2 at the ER has been suggested earlier as Bcl-2 may regulate calcium fluxes between the ER and the mitochondria. For a review on Ca2+ and its role in apoptosis. |
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| Regulatory Status: | RUO - Research Use Only |
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