| Alternative Name: | Fatty acid binding protein 1, FABPL, L-FABP, Liver-type fatty acid binding protein, Z protein |
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| Clone: | 2G4 |
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| Host: | Mouse |
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| Isotype: | IgG1κ |
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| Immunogen: | Recombinant human FABP1/L-FABP (aa 1-127) purified from E. coli. |
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| UniProt ID: | P07148 |
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| Source: | Purified from hybridoma tissue culture supernatant. |
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| Species reactivity: | Human
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| Applications: | ELISA, ICC, IF, WB
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| Formulation: | Liquid. In PBS, pH 7.4, containing 0.02% sodium azide and 10% glycerol. |
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| Handling: | Avoid freeze/thaw cycles. |
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| Shipping: | Blue Ice |
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| Long Term Storage: | -20°C |
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| Scientific Background: | FABP1 (Fatty acid binding protein1) encodes the fatty acid binding protein found in liver. FABP1 is composed of ten antiparallelbeta strands that form a barrel with a bigger binding pocket than the other FABPs allowing it to accommodate two fatty acid. This protein binds free fatty acids and their coenzyme A derivatives, bilirubin, and some other small molecules in the cytoplasm; it may be involved in intracellular lipid transport and metabolism. |
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| Regulatory Status: | RUO - Research Use Only |
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The recombinant protein (50ng) and cell lysates (40µg) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human FABP1/L-FABP antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1: Recombinant human FABP1/L-FABP protein, Lane 2: HepG2 cell lysate, Lane 3: Mouse liver tissue lysate
ICC/IF analysis of FABP1/L-FABP in Hep3B cells. The cell was stained with ATGA0521
(1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).
