| Alternative Name: | Natural killer cells antigen CD94, KP43, Killer cell lectin-like receptor subfamily D member 1, NK cell receptor |
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| Clone: | AT13E3 |
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| Host: | Mouse |
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| Isotype: | IgG1κ |
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| Immunogen: | Recombinant human KLRD1 (aa 32-179) purified from E. coli. |
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| UniProt ID: | Q13241 |
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| Source: | Purified from ascites. |
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| Species reactivity: | Human
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| Applications: | ELISA, Flow Cytometry, WB
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| Purity Detail: | Protein A affinity purified |
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| Formulation: | Liquid. In PBS, pH 7.4, containing 0.02% sodium azide and 10% glycerol. |
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| Handling: | Avoid freeze/thaw cycles. |
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| Shipping: | Shipped on Blue Ice |
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| Long Term Storage: | -20°C |
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| Scientific Background: | The protein encoded by CD94 gene is a lectin, cluster of differentiation and a receptor that is involved in cell signaling and is expressed on the surface of natural killer cells in the innate immune system. CD94 pairs with the NKG2 molecule as a heterodimer. The CD94/NKG2 complex, on the surface of natural killer cells interacts with Human Leukocyte Antigen (HLA) -E on target cells. |
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| Regulatory Status: | RUO - Research Use Only |
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Flow cytometry analysis of KLRD1 in PBMC cells. The cell was stained with ENZ-ABS723 at 2-5µg for 1x106cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).
The cell lysates (40µg) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human KLRD1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1: K562 cell lysate. Lane 2: MCF7 cell lysate. Lane 3: A549 cell lysate.
